[1].Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia[J].Asian Herpetological Research,2022,13(1):23-33.[doi:10.16373/j.cnki.ahr.200125]
　Qiyu LI,Tingting ZOU,Wenqi TANG,et al.Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia[J].Asian Herpetological Research(AHR),2022,13(1):23-33.[doi:10.16373/j.cnki.ahr.200125]
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Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia()
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

卷:
13
期数:
2022年1期
页码:
23-33
栏目:
出版日期:
2022-03-25

文章信息/Info

Title:
Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia
文章编号:
AHR-2020-0125
Author(s):
Qiyu LI1; 2;  Tingting ZOU1; 2;  Wenqi TANG1; 2;  Yang WANG3;  Weiguo DU1 and Xifeng WANG1*
1 Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
3 Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China
Keywords:
geographic variation;  maternal thermal environment;  reptile;  sex chromosome;  sex determination;  sex ratio;  sex reversal
DOI:
10.16373/j.cnki.ahr.200125
Abstract:
Sex-determining systems show a striking diversity not only among species, but also among populations. In reptiles, sex-determination is a continuum, from temperature-dependent sex determination (TSD) to genetic sex determination (GSD). The multi-ocellated racerunner (Eremias multiocellata) is reported to be a cryptic ZZ/ZW chromosomal TSD species, with male-biased sex ratios at high temperatures in two Gansu populations. However, the generality of the sex-determining pattern in different populations of this species remains unclear. To investigate the mode of sex determination in a population of E. multiocellata from the desert steppe of Inner Mongolia, we first identified sex chromosomes via comparative genomic hybridization (CGH). We then conducted a thermal manipulation experiment to determine the effect of gestation temperature on offspring sex ratios. From the CGH studies we found that lizards from the Inner Mongolia population possessed ZZ/ZW sex chromosomes. However, our thermal manipulation experiment showed that gestation temperature did not affect the sex ratio of neonates in this population. In combination, these results rule out TSD in the Inner Mongolia population of E. multiocellata, and suggest that there is widespread geographic variation in the sex-determining system of this species.

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更新日期/Last Update: 2022-03-25