[1].A New Leaf Litter Toad of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Sichuan Province, China with Supplementary Description of L. oshanensis[J].Asian Herpetological Research,2021,12(2):143-166.[doi:10.16373/j.cnki.ahr.200118]
　Shengchao SHI,Yinmeng HOU,Zhaobin SONG,et al.A New Leaf Litter Toad of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Sichuan Province, China with Supplementary Description of L. oshanensis[J].Asian Herpetological Research(AHR),2021,12(2):143-166.[doi:10.16373/j.cnki.ahr.200118]
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A New Leaf Litter Toad of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Sichuan Province, China with Supplementary Description of L. oshanensis()
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

卷:
12
期数:
2021年2期
页码:
143-166
栏目:
出版日期:
2021-06-25

文章信息/Info

Title:
A New Leaf Litter Toad of Leptobrachella Smith, 1925 (Anura, Megophryidae) from Sichuan Province, China with Supplementary Description of L. oshanensis
文章编号:
AHR-2020-0118
Author(s):
Shengchao SHI1; 2; 3;  Yinmeng HOU1; 2; 3;  Zhaobin SONG2;  Jianping JIANG1*;  Bin WANG1*
1 CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2 Key Laboratory of Bio-Resources and Eco-Environment of Ministry of Education, College of Life Sciences, Sichuan University, Chengdu 610065, Sichuan, China
3 University of Chinese Academy of Sciences, Beijing 100049, China
Keywords:
multiple data;  new species;  sympatric distribution;  taxonomy
DOI:
10.16373/j.cnki.ahr.200118
Abstract:
A new species of the Asian leaf litter toad genus Leptobrachella is described from Sichuan Province, China. Molecular phylogenetic analyses based on mitochondrial and nuclear gene sequences clustered the new species as an independent clade nested into L. oshanensis species group. The new species could be distinguished from its congeners by a combination of following characters: body size moderate (25.8–32.6 mm in male, 33.7–34.1 mm in female); distinct black spots present on flanks; toes rudimentary webbed, with narrow lateral fringes, dermal ridges under toes interrupted at articulations; ventral belly cream white with variable brown specking; skin on dorsum relatively smooth with fine tiny granules or short ridges; iris copper above, silver bellow; greyish black patches on posterior thigh absent or small; spines on surface of chest absent in male during breeding season; nasals entirely or partially separated from sphenethmoid in male; dorsal surface of tadpoles semitransparent light brown, spots on tail absent, keratodont row formula I: 3+3(2+2)/2+2: I; calls simple, call series basically consist of repeated long calls, at dominant frequency （4831.9 ± 155.8） Hz and call duration （544.5 ± 146.8） ms. In addition, we made supplementary description on L. oshanensis including holotype, variations, tadpoles, skull and bioacoustics. Besides, this paper reports cases of femoral adipose glands in the genus Leptobrachella as first known sexual dimorphism skin glands for males of Megophryidae.

参考文献/References:

Anderson J. 1871. A list of the reptilian accession to the Indian Museum, Calcutta from 1865 to 1870, with a description of some new species. J Asiat Soc Bengal Sci., 40: 1239
Bioacoustics Research Program. 2013. Raven Pro: Interactive sound analysis software. Version 1.5. http://www.birds.cornell.edu/raven
Boersma P. 2001. Praat, a system for doing phonetics by computer. Glot International 5: 341–345
Boulenger G. A. 1893. Concluding report on the reptiles and batrachians obtained in Burma by Signor L. Fea dealing with the collection made in Pegu and the Karin Hills in 1887–88. Annali del Museo Civico di Storia Naturale di Genova, 13: 304–347
Boulenger G. A. 1900. Descriptions of new batrachians and reptiles from the Larut Hills, Perak. Ann Mag Nat Hist, 6: 186–194
Castresana J. 2000. Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. Mol Biol Evol, 17(4): 540–552
Chen J. M., Poyarkov N. J., Suwannapoom C., Lathrop A., Wu Y. H., Zhou W. W., Yuan Z. Y., Jin J. Q., Chen H. M., Liu H. Q., Nguyen T. Q., Nguyen S. N., Duong T. V., Eto K., Nishikawa K., Matsui M, Orlov N. L., Stuart B. L., Brown R. M., Rowley J., Murphy R. W., Wang Y. Y., Che J. 2018. Large-scale phylogenetic analyses provide insights into unrecognized diversity and historical biogeography of Asian leaf-litter frogs, genus Leptolalax (Anura: Megophryidae). Mol Phylogenet Evol, 124: 162–171
Chen J. M., Xu K., Poyarkov N. J., Wang K., Yuan Z. Y., Hou M., Suwannapoom C., Wang J., Che J. 2020. How little is known about “the little brown frogs”: description of three new species of the genus Leptobrachella (Anura: Megophryidae) from Yunnan Province, China. Zool Res, 41: 1–22
Chen W. C., Liao X., Zhou S. C., Mo Y. M. 2019. A new species of Leptobrachella (Anura: Megophryidae) from southern Guangxi, China. Zootaxa, 4563: 67–417
Darriba D., Taboada G. L., Doallo R., Posada D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat methods, 9: 772
Das I., Tron R. K. L., Rangad D., Hooroo R. N. 2010. A new species of Leptolalax (Anura: Megophryidae) from the sacred groves of Mawphlang, Meghalaya, north-eastern India. Zootaxa, 2339: 44–56
Dehling J. M. 2012a. Redescription of Leptolalax gracilis (Günther, 1872) from Borneo and taxonomic status of two populations of Leptolalax (Anura: Megophryidae) from Peninsular Malaysia. Zootaxa, 3328: 20–34
Dehling J. M. 2012b. Eine neue Art der Gattung Leptolalax (Anura: Megophryidae) vom Gunung Benom, Westmalaysia/A new species of the genus Leptolalax (Anura: Megophryidae) from Gunung Benom, Peninsular Malaysia. Sauria, 34: 9–21
Dehling J. M., Matsui M. 2013. A new species of Leptolalax (Anura: Megophryidae) from Gunung Mulu National Park, Sarawak, East Malaysia (Borneo). Zootaxa, 3670: 33–44
Dring J. 1983. Frogs of the genus Leptobrachella (Pelobatidae). Amphibia-Reptillia, 4: 89–102
Dubois A. 1983. Note preliminaire sur le genre Leptolalax Dubois, 1980 (Amphibiens, Anoures), avec diagnose d’une espece novelle du Vietnam. Alytes, 2: 147–153
Dubois A., Grosjean S., Ohler A., Adler K., Zhao E. M. 2010. The nomenclatural status of some generic nomina of Megophryidae (Amphibia, Anura). Zootaxa, 2493: 66–68
Duong T. V., Do D. T., Ngo C. D., Nguyen T. Q., N. Poyarkov A. Jr. 2018. A new species of the genus Leptolalax (Anura: Megophryidae) from southern Vietnam. Zool Res, 39: 181–196
Eto K., Matsui M., Hamidy A., Munir M., Iskandar D. T. 2018. Two new species of the genus Leptobrachella (Amphibia: Anura: Megophryidae) from Kalimantan, Indonesia. Curr Herpetol, 37(2): 95–105
Eto K., Matsui M., Nishikawa K. 2015. Description of a new species of the genus Leptobrachella (Amphibia, Anura, Megophryidae) from Borneo. Curr Herpetol, 34(2): 128–139
Eto K., Matsui M., Nishikawa K. 2016. A new highland species of dwarf litter frog genus Leptobrachella (Amphibia, Anura, Megophryidae) from Sarawak. Raffles Bull Zool. Singapore, 64: 194–203
Fei L. 1999. Atlas of amphibians of China. Zhengzhou: Henan Press of Science and Technology (In Chinese)
Fei L., Hu C., Ye C. Y., Huang Y. 2009. Fauna Sinica. Amphibia. Volume 2. Anura. Beijing: Chinese Academy of Science, Science Press [In Chinese]
Fei L., Ye C. Y. 1992. Comments on the taxonomy of pelobatid toads of genus Leptolalax (Carpophrys) with description of a new species. Acta Zool Sin, 38: 245–253 (In Chinese with English abstract)
Fei L., Ye C. Y. 2005. The key and illustration of Chinese. Sichuan Publishing House of Science and Technology. Chongqing (In Chinese)
Fei L., Ye C. Y. 2016. Amphibians of China, Volume 1. Science Press. Beijing, China
Fei L., Ye C. Y., Huang Y. Z. 1990. Key to Chinese amphibians. Publishing House for Scientific and Technological Literature. Chongqing [In Chinese]
Fei L., Ye C. Y., Jiang J. P. 2012. Colored atlas of Chinese amphibians and their distributions. Sichuan Publishing House of Science and Technology, Chengdu, China (In Chinese)
Frost D. R. 2020. Amphibian Species of the World: an Online Reference. Version 6.1. Electronic Database accessible. American Museum of Natural History, New York. Retrieved from https://amphibiansoftheworld.amnh.org/index.php
Fu J. Z., Weadick C. J., Bi K. 2007. A phylogeny of the high-elevation Tibetan megophryid frogs and evidence for the multiple origins of reversed sexual size dimorphism. J Zool, 273: 315–325
Gong Y. Z., Zeng Y. W., Zheng P. Y., Liao X., Xie F. 2020. Structural and bio-functional assessment of the postaxillary gland in Nidirana pleuraden (Amphibia: Anura: Ranidae). Zool Letters, 6: 7
Grismer L. L., Grismer J. L., Youmans T. M. 2004. A new species of Leptolalax (Anura: Megophryidae) from Pulau Tioman, West Malaysia. Asiatic Herpetol Res, 10: 8–11
Guindon S., Dufayard J. F., Lefort V., Anisimova M., Hordijk W., Gascuel O. 2010. New algorithms and methods to estimate maximum-likelihood phylogenies: assessing the performance of PhyML 3.0. Syst Biol, 59(3): 07–321
Günther A. 1872. On the reptiles and amphibians of Borneo. Proc Zool Soc Lond, 1872: 586–600
Günther A. 1895. Te reptiles and batrachians of the Natuna Islands. Novitates Zoologicae, 2: 499–502
Hall T. A. 1999. BIOEDIT: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 41(41): 95–98
Hoang C. V., Nguyen T. T., Luu V. Q., Nguyen T. Q., Jiang J. P. 2019. A new species of Leptobrachella Smith 1925 (Anura: Megophryidae) from Thanh Hoa Province, Vietnam. Raffles Bull Zool, 67: 536–556
Hou Y. M., Zhang M. F., Hu F., Li S. Y., Shi S. C., Chen J., Mo X. Y., Wang B. 2018. A new species of the genus Leptolalax (Anura, Megophryidae) from Hunan, China. Zootaxa, 4444(3): 247–266
Humtsoe L. N., Bordoloi S., Ohler A., Dubois A. 2008. Rediscovery of a long known species, Ixalus lateralis Anderson, 1871. Zootaxa, 1921: 24–34
Inger R. F, Orlov N., Darevsky I. 1999. Frogs of Vietnam: a report on new collections. Fieldiana Zool, 92: 1–46
Inger R. F., Lakim M., Biun A., Yambun P. 1997. A new species of Leptolalax (Anura: Megophryidae) from Borneo. Asiatic Herpetol Res, 7: 48–50
Inger R. F., Stuebing R. B. 1992 “1991”. A new species of frog of the genus Leptobrachella Smith (Anura: Pelobatidae), with a key to the species from Borneo. Raffles Bull Zool, 39: 99–103
Inger R. F., Stuebing R. B., Tan F. 1995. New species and new records of anurans from Borneo. Raffles Bull Zool, 43: 115–132
Jiang K., Yan F., Suwannapoom C., Chomdej S., Che J. 2013. A new species of the genus Leptolalax (Anura: Megophryidae) from northern Thailand. Asian Herpetol Res, 4: 100–108
K?hler J., Jansen M., Rodríguez A., Kok P., Toledo L. F., Emmrich M., Glaw F., Haddad C. F. B., R?del M. O., Vences M. 2017. The use of bioacoustics in anuran taxonomy: theory, terminology, methods and recommendations for best practice. Zootaxa, 4251(1): 1–124
Kumar S., Stecher G., Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis Version 7.0 for Bigger Datasets. Mol Biol Evol, 33(7): 1870
Lannoo M. J. 1987. Neuromast topography in anuran amphibians. J Morphol, 191: 115–129
Lathrop A., Murphy R. W., Orlov N., Ho C. T. 1998. Two new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Amphibia-Reptilia, 19: 253–267
Li S. Z., Liu J., Wei G., Wang B. 2020. A new species of the Asian leaf litter toad genus Leptobrachella (Amphibia, Anura, Megophryidae) from southwest China. ZooKeys, 943: 91–118
Liu C. C, Hu S. C. 1961. The tailless amphibians of China. Science Press, Beijing, China
Liu C. C. 1950. Amphibians of Western China. Fieldiana: Zoological Memoirs, 2, 1–400, 10 pl.
Luo T., Xiao N., Gao K., Zhou J. 2020. A new species of Leptobrachella (Anura, Megophryidae) from Guizhou Province, China. ZooKeys, 923: 115–140
Mahony S., Foley N. M., Biju S. D., Teeling E. C. 2017. Evolutionary history of the Asian horned frogs (Megophryinae): integrative approaches to timetree dating in the absence of a fossil record. Mol Biol Evol, 34(3): 744–771
Malkmus R. 1992. Leptolalax pictus sp. nov. (Anura: Pelobatidae) vom Mount Kinabalu/NordBorneo. Sauria 14: 3–6.
Mathew R., Sen N. 2009. Description of a new species of Leptobrachium Tschudi, 1838 (Amphibia: Anura: Megophryidae) from Meghalaya, India. Rec Zool Surv India, 109: 91–108
Matsui M. 1997. Call characteristics of Malaysian Leptolalax with a description of two new species (Anura: Pelobatidae). Copeia, 1997: 158–165
Matsui M. 2006. Three new species of Leptolalax from Thailand (Amphibia, Anura, Megophryidae). Zool Sci, 23(9): 821–830
Matsui M., Belabut D. M., Ahmad N., Yong H. S. 2009. A new species of Leptolalax (Amphibia, Anura, Megophryidae) from Peninsular Malaysia. Zool Sci, 26(3): 243–247
Matsui M., Nishikawa K., Yambun P. 2014a. A new Leptolalax from the mountains of Sabah, Borneo (Amphibia, Anura, Megophryidae). Zootaxa, 3753(3): 440–452
Matsui M., Zainudin R., Nishikawa K. 2014b. A new species of Leptolalax from Sarawak, Western Borneo (Anura: Megophryidae). Zool Sci, 31(11): 773–779
Mauro D. S., Gower D. J., Oommen O. V., Wilkinson M., Zardoya R. 2004. Phylogeny of caecilian amphibians (Gymnophiona) based on complete mitochondrial genomes and nuclear RAG1. Mol Phylogenet Evol, 33: 413–427
Mo Y. M., Jiang J. P., Ye C. Y. 2008. A new record of frogs from China:Paramegophrys (Paramegophrys) sungi (Megophryidae, Amphibia). J Anhui Norm Univ (Nat Sci Ed), 31(4): 368–370
Nguyen L.T., Poyarkov N.A. Jr, Le D.T., Vo B.D., Ninh H.T., Duong T.V., Murphy R.W., Sang N.V. 2018. A new species of Leptolalax (Anura: Megophryidae) from Son Tra Peninsula, central Vietnam. Zootaxa, 4388: 1–21
Ohler A., Marquis O., Swan S., Grosjean S. 2000. Amphibian biodiversity of Hoang Lien Nature Reserve (Lao Cai Province, northern Vietnam) with description of two new species. Herpetozoa, 13(1/2): 71–87
Ohler A., Wollenberg K. C., Grosjean S., Hendrix R., Vences M., Ziegler T., Dubois A. 2011. Sorting out Lalos: description of new species and additional taxonomic data on megophryid frogs from northern Indochina (genus Leptolalax, Megophryidae, Anura). Zootaxa, 3147: 1–83
Poyarkov N. J, Rowley J. J., Gogoleva S. I., Vassilieva A. B., Galoyan E. A., Orlov N. L. 2015. A new species of Leptolalax (Anura: Megophryidae) from the western Langbian Plateau, southern Vietnam. Zootaxa, 3931(2): 221–252
Qian T. Y., Xiao X., Cao Y., Xiao N. W., Yang D. D. 2020. A new species of Leptobrachella (Anura: Megophryidae) Smith, 1925 from Wuling Mountains in Hunan Province, China. Zootaxa, 4816: 491–526
Ronquist F., Teslenko M., Paul V. D. M., Ayres D. L., Darling A., H?hna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. 2012. Mrbayes 3.2: efficient bayesian phylogenetic inference and model choice across a large model space. Syst Biol, 61(3): 539–542
Rowley J. J., Cao T. T. 2009. A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 2198: 51–60
Rowley J. J., Dau V. Q., Hoang H. D., Le D. T. T., Cutajar T. P., Nguyen T. T. 2017a. A new species of Leptolalax (Anura: Megophryidae) from northern Vietnam. Zootaxa, 4243: 544–564
Rowley J. J., Dau V. Q., Nguyen T. T. 2013. A new species of Leptolalax (Anura: Megophryidae) from the highest mountain in Indochina. Zootaxa, 3737(4): 415–428
Rowley J. J., Hoang H. D., Dau V. Q., Le T. T. D., Cao T. T. 2012. A new species of Leptolalax (Anura: Megophryidae) from central Vietnam. Zootaxa, 3321: 56–68
Rowley J. J., Le D. T. T., Tran D. T. A., Hoang D. H. 2011. A new species of Leptobrachella (Anura: Megophryidae) from southern Vietnam. Zootaxa, 2796: 15–28
Rowley J. J., Stuart B. L., Neang T., Emmett D. A. 2010c. A new species of Leptolalax (Anura: Megophryidae) from northeastern Cambodia. Zootaxa, 2567: 57–68
Rowley J. J., Stuart B. L., Neang T., Hoang H. D., Dau V. Q., Nguyen T. T., Emmett D. A. 2015. A new species of Leptolalax (Anura: Megophryidae) from Vietnam and Cambodia. Zootaxa, 4039: 401–417
Rowley J. J., Stuart B. L., Richards S. J., Phimmachak S., Sivongxay N. 2010a. A new species of Leptolalax (Anura: Megophryidae) from Laos. Zootaxa, 2681: 35–46
Rowley J. J., Tran D. T. A., Le D. T. T., Dau V. Q., Peloso P. L. V., Nguyen T. Q., Hoang H. D., Nguyen T. T., Ziegler T. 2016. Five new, microendemic Asian Leaf-litter Frogs (Leptolalax) from the southern Annamite mountains, Vietnam. Zootaxa, 4085: 63–102
Rowley J. J., Dau V. Q., Cao T.T. 2017b. A new species of Leptolalax (Anura: Megophryidae) from Vietnam. Zootaxa, 4273(1): 61–79
Rowley J. J., Hoang D. H., Le T. T. D., Dau Q. V., Cao T. T. 2010b. A new species of Leptolalax (Anura: Megophryidae) from Vietnam and further information on Leptolalax tuberosus. Zootaxa, 2660: 33–45
Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press
Sengupta S., Sailo S., Lalremsanga H. T., Das A., Das I. 2010. A new species of Leptolalax (Anura: Megophryidae) from Mizoram, north-eastern India. Zootaxa, 2406: 56–68
Sichuan Biological Research Institute. 1977. The systematic key to Chinese Amphibia. Science Press, Beijing, China
Simon C., Frati F., Beckenbach A., Crespi B., Liu H., Flook P. 1994. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am, 87(6): 651–701
Stuart B. L., Rowley J. J. 2020. A new Leptobrachella (Anura: Megophryidae) from the Cardamom Mountains of Cambodia. Zootaxa, 4834: 556–572
Sung Y. H., Yang J. H., Wang Y. Y. 2014. A new species of Leptolalax (Anura: Megophryidae) from southern China. Asian Herpetol Res, 5(2): 80–90
Taylor E. H. 1962. The amphibian fauna of Thailand. Univ Kans Sci Bull, 43: 265–599
Thomas E. O., Tsang L., Licht P. 1993. Comparative histochemistry of the sexually dimorphic skin glands of anuran amphibians. Copeia, 1993: 133–143
Wang J., Li Y. L., Li Y., Chen H. H., Zeng Y. J., Shen J. M., Wang Y. Y. 2019. Morphology, molecular genetics, and acoustics reveal two new species of the genus Leptobrachella from northwestern Guizhou Province, China (Anura, Megophryidae). ZooKeys, 848: 119–154
Wang J., Yang J. H., Li Y., Lyu Z. T., Zeng Z. C., Liu Z. Y., Ye Y. H., Wang Y. Y. 2018. Morphology and molecular genetics reveal two new Leptobrachella species in southern China (Anura, Megophryidae). ZooKeys, 776: 105– 137
Xiang S. J., Deng X. J., Dong C., Xiao Z. L. 2008. A new record of amphibian in Hunan Province Leptolalax oshanensis. Chinese J Zool, 43(2): 138–140 (In Chinese with English abstract)
Yang J. H., Wang Y. Y., Chen G. L., Rao D. Q. 2016. A new species of the genus Leptolalax (Anura: Megophryidae) from Mt. Gaoligongshan of western Yunnan Province, China. Zootaxa, 4088: 379–394
Yang J. H., Zeng Z. C., Wang Y. Y. 2018. Description of two new sympatric species of the genus Leptolalax (Anura: Megophryidae) from western Yunnan of China. PeerJ, 6(e4586): 1–32
Yuan Z. Y., Sun R. D., Chen J. M., Rowley J. J., Wu Z. J., Hou S. B., Wang S. N., Che J. 2017. A new species of the genus Leptolalax (Anura: Megophryidae) from Guangxi, China. Zootaxa, 4300: 551–570

更新日期/Last Update: 2021-06-25