[1].Genetic Differentiation of the Forest Crested Lizards, Calotes emma alticristatus Schmidt, 1925 and C. emma emma Gray, 1845 (Squamata: Agamidae) Relating to Climate Zones in Thailand[J].Asian Herpetological Research,2020,11(1):19-27.[doi:10.16373/j.cnki.ahr.190054]
 Weerachai SAIJUNTHA,Chairat TANTRAWATPAN*,Warayutt PILAP,et al.Genetic Differentiation of the Forest Crested Lizards, Calotes emma alticristatus Schmidt, 1925 and C. emma emma Gray, 1845 (Squamata: Agamidae) Relating to Climate Zones in Thailand[J].Asian Herpetological Research(AHR),2020,11(1):19-27.[doi:10.16373/j.cnki.ahr.190054]

Genetic Differentiation of the Forest Crested Lizards, Calotes emma alticristatus Schmidt, 1925 and C. emma emma Gray, 1845 (Squamata: Agamidae) Relating to Climate Zones in Thailand()

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]



Genetic Differentiation of the Forest Crested Lizards, Calotes emma alticristatus Schmidt, 1925 and C. emma emma Gray, 1845 (Squamata: Agamidae) Relating to Climate Zones in Thailand
Weerachai SAIJUNTHA1 Chairat TANTRAWATPAN2* Warayutt PILAP1 Sutthira SEDLAK1 Khathalee SAKDAKHAM1 Paradee SRIPIROM1 Warong SUKSAVATE3 Watee KONGBUNTAD4 and Wittaya TAWONG5
1 Walai Rukhavej Botanical Research Institute, Biodiversity and Conservation Research Unit, Mahasarakham University, Maha Sarakham 44150, Thailand
2 Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani 12120, Thailand
3 Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok 10900, Thailand
4 Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand
5 Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
Agamid Reptile Genetic diversity Phylogeny CO1 Haplotype
In Thailand, the forest crested lizard, Calotes emma consists of two subspecies, C. emma alticristatus Schmidt, 1925 and C. emma emma Gray, 1845. This study was performed to determine the genetic diversity and differentiation of C. emma from 16 different localities throughout Thailand. A total of 116 samples were analyzed using the mitochondrial cytochrome c oxidase subunit 1 (CO1). Of these, 65 and 51 of C. e. alticristatus and C. e. emma were classified into 23 (N1 – N23) and 21 (S1 – S21) haplotypes, respectively. There was no shared haplotype between subspecies or between different populations within each subspecies. These haplotypes were classified into four (north-A to north-D) and three (south-A to south-C) haplogroups of C. e. alticristatus and C. e. emma, respectively. Phylogenetic analyses retrieved four lineages (classified as I to IV). Lineages I and II contained the four haplogroups of C. e. alticristatus, whereas lineage III and IV contained three haplogroups of C. e. emma. These two subspecies live separately in different climate zones, i.e. C. e. alticristatus is found in an equatorial winter dry climate, whereas C. e. emma inhabits areas with an equatorial monsoonal climate.


Bandelt H. J., Forster P., Rohl A. 1999 Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol, 16: 37–48
Bennett D. 1999. Expedition field techniques: reptiles and amphibians. Geography Outdoors, London
Bougeard S., Dray S. 2018. Supervised Multiblock Analysis in R with the ade4 Package. J Stat Softw, 86(1): 1–17
Chan-ard T., Parr J. W. K., Nabhitabhata J. 2015. A field guide to the reptiles of Thailand. Oxford University Press, New York
Cheptou P. O., Hargreaves A. L., Bonte D., Jacquemyn H. 2017. Adaptation to fragmentation: evolutionary dynamics driven by human influences. Philos Trans R Soc Lond Ser B Biol Sci, 372(1712): 20160037
Conner J. K., Hartl D. L. 2004. A primer of ecological genetics. Sunderland: Sinauer Associates Incorporated
Cox M. J., van Dijk P. P., Nabhitabhata J., Thirakhupt K. 1998. A photographic guide to snakes and other reptiles of Peninsular Malaysia, Singapore and Thailand. New Holland Publishers (UK) Ltd
Das I. 2010. A field guide to the reptiles of Thailand and South-East Asia. Tien Wah Press, Hong Kong
Eckert C., Samis K., Lougheed S. 2008. Genetic variation across species’ geographical ranges: the central–marginal hypothesis and beyond. Mol Ecol, 17(5): 1170–1188
Excoffier L., Lischer H. E. 2010. Arlequin suite ver 3.5: a new series of programs to perform population genetics analyses under Linux and Windows. Mol Ecol Res, 10(3): 564–567
Hall T. 1999. BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symp Ser, 41: 95–98
Hartmann T., Geissler P., Poyarkov N., Ihlow F., Galoyan E., Rodder D., Bohme W. 2013. A new species of the genus Calotes Cuvier, 1817 (Squamata: Agamidae) from southern Vietnam. Zootaxa, 3599: 246–260
Hawkeswood T. J., Sommung B. 2017. A record of the Forest Crested Lizard, Calotes emma Gray, 1835 (Reptilia: Agamidae) from the Doi Inthanon National Park, Chiang Mai Province, northern Thailand, with an account of dog attacks on the lizard. Calodema, 569: 1–4
Huang Y., Guo X., Ho S. Y. W., Shi H., Li J., Li J., Cai B., Wang Y. 2013. Diversification and demography of the Oriental garden lizard (Calotes versicolor) on Hainan Island and the adjacent mainland. PLoS One, 8: e64754
Kottek M., Grieser J., Beck C., Rudolf B., Rubel F. 2006. World Map of the Koppen-Geiger climate classification updated. Meteorol Z, 15: 259–263
Krishnan S. 2008. New species of Calotes (Reptilia: Squamata: Agamidae) form the Southern Western Ghats, India. J Herpetol, 42(3): 530–535 ooooo
Kritpetcharat O., Kritpetcharat C., Luangpirom A., Watcharanon P. 1999. Karyotype of four Agamidae species from the Phu Phan National Park in Thailand. Sci Asia, 25: 185–188
Kumar S., Stecher G., Li M., Knyaz C., Tamura K. 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Mol Biol Evol, 35(6): 1547–1549
Larkin M. A., Blackshields G., Brown N. P., Chenna R., McGettigan P. A., McWilliam H., Valentin F., Wallace I. M., Wilm A., Lopez R., Thompson J. D., Gibson T. J., Higgins D. G. 2007. Clustal W and Clustal X version 2.0. Bioinformatics, 23(21): 2947–2948
Librado P., Rozas J. 2009. DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11): 1451–1452
Majumder J., Agarwala B. K. 2015. Calotes emma Gray, 1845 (Squamata: Agamidae): range extension and new addition to the reptilian fauna of Tripura, northeast India. Check List, 11(2): 1652
Mantel N. 1967. The detection of disease clustering and a generalized regression approach. Cancer Res, 27: 209–220
Nylander J. A. A. 2008. MrModeltest v2.3 Program distributed by the author. Evolutionary Biology Centre. Uppsala University
R Core Team. 2013. R: A language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, Austria
Ronquist F., Huelsenbeck J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1572–1574
Saijuntha W., Khumkratok S., Wongpakam K., Thanonkeo S., Senakhun C., Appamaraka S., Yodsiri S., Thongnetr W., Pilap W., Kongbuntad W., Tawong W., Agatsuma T., Petney T. N., Tantrawatpan, C. 2017. Genetic diversity and population structure of Blue-crested lizard, Calotes mystaceus Duméril & Bibron, 1837 (Squamata: Agamidae) in Thailand. J Genet, 96(2): 377–382
Sen Y. H., Hashim R., Zaleha S., Diah M., Belabut D., Azirun M. D., Liat L. B. 2009. Calotes emma alticristatus (Reptilia, Squamata: Agamidae) in Kelantan, Peninsular Malaysia. J Sci Technol Trop, 5(1): 35–37
Seyahooei M. A., van Alphen J. J. M., Kraaijeveld K. 2011. Genetic structure of Leptopilina boulardi populations from different climatic zones of Iran. BMC Ecol, 11: 4
Spawls S., Howell K., Kinkel H., Menegon M. 2018. Field Guide to East African Reptiles. Bloomsbury, 624 pp
Teynié A. 2004. Notes on Retiles of Nam Lan Conservation Area in Phongsaly Province of Lao PDR. Technical Report: Société d’Histoire Naturelle, Aydat, France
Vindum J. V., Win H., Thin T., Lwin K. S., Shein A. K., Tun H. 2003. A new Calotes (Squamata: Agmidae) from the Indo-Burman range of western Myanmar (Burma). Proc Calif Acad Sci, 54(1): 1–16
Zug G. R., Brown H. H. K., Schulte II J. A., Vindum J. V. 2006. Systematics of the Garden Lizards, Calotes versicolor Group (Reptilia, Squamata, Agamidae), in Myanmar: Central Dry Zone Populations. Proc Calif Acad Sci, 57(2): 35–68

更新日期/Last Update: 2020-03-27