[1].Reproductive Isolating Mechanisms in the Bangladesh Coastal Bullfrog Hoplobatrachus litoralis and Its Congeneric Species Revealed by Crossing Experiments and Examination on Spermatogenesis of the Hybrids[J].Asian Herpetological Research,2017,8(1):27-38.[doi:10.16373/j.cnki.ahr.160026]
　Mahmudul HASAN*,Mohammed Mafizul ISLAM,Md. Mukhlesur Rahman KHAN,et al.Reproductive Isolating Mechanisms in the Bangladesh Coastal Bullfrog Hoplobatrachus litoralis and Its Congeneric Species Revealed by Crossing Experiments and Examination on Spermatogenesis of the Hybrids[J].Asian Herpetological Reserch(AHR),2017,8(1):27-38.[doi:10.16373/j.cnki.ahr.160026]
点击复制

Reproductive Isolating Mechanisms in the Bangladesh Coastal Bullfrog Hoplobatrachus litoralis and Its Congeneric Species Revealed by Crossing Experiments and Examination on Spermatogenesis of the Hybrids()
分享到：

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

卷:
8
期数:
2017年1期
页码:
27-38
栏目:
出版日期:
2017-03-25

文章信息/Info

Title:
Reproductive Isolating Mechanisms in the Bangladesh Coastal Bullfrog Hoplobatrachus litoralis and Its Congeneric Species Revealed by Crossing Experiments and Examination on Spermatogenesis of the Hybrids
文章编号:
AHR-2016-0026
Author(s):
Mahmudul HASAN1*;  Mohammed Mafizul ISLAM1;  Md. Mukhlesur Rahman KHAN2;  Ratanasate WANICHANON3;  Atsushi KURABAYASHI1 and Masayuki SUMIDA1; 4
1 Amphibian Research Center, Hiroshima University, 1-3-1 Kagamiyama, Higashihiroshima 739-8526, Japan
2 Department of Fisheries Biology and Genetics, Bangladesh Agricultural University, Mymensingh-2202, Bangladesh
3 Department of Anatomy, Phramonkutklao Medical College, Rajawithi Rd., Bangkok 10400, Thailand
4 1-6-15 Ushitaasahi, Higashi ku, Hiroshima 732-0067, Japan
Keywords:
Hoplobatrachus;  reproductive isolation;  interspecific hybrids;  spermatogenesis;  meiotic chromosomes
DOI:
10.16373/j.cnki.ahr.160026
Abstract:
To elucidate reproductive isolating mechanisms in the Bangladesh coastal bullfrog Hoplobatrachus litoralis and its congeneric species, we performed crossing experiments using three species: H. litoralis, H. tigerinus and H. rugulosus. In addition, we conducted histological observations on spermatogenesis of the hybrids. The reciprocal hybrids between H. litoralis and H. tigerinus developed normally with somewhat lower viability at the metamorphosis stage compared with the controls. Most of the metamorphosed frogs became mature. On the other hand, almost all hybrids between female H. rugulosus and male H. litoralis or H. tigerinus died of underdevelopment at the tadpole stage, and only a few hybrids metamorphosed normally and survived to maturity. The inner structures of the testes of the control H. litoralis and H. tigerinus were completely normal, with seminiferous tubules filled with compact bundles of normal spermatozoa. Those of the reciprocal hybrids between H. litoralis and H. tigerinus were almost normal or slightly abnormal, with seminiferous tubules that contained pycnotic nuclei in addition to normal bundles of normal spermatozoa, which demonstrates slight abnormalities in spermatogenesis. In contrast, the hybrids between female H. rugulosus and male H. litoralis or H. tigerinus had no bundles of spermatozoa nor spermatids in the seminiferous tubules, which indicates entirely abnormal spermatogenesis. Meiotic chromosome figures in the reciprocal hybrids between H. litoralis and H. tigerinus showed slight abnormalities, with the occurrence of univalents and increase of rod-shaped bivalents. These results indicate that H. litoralis and H. tigerinus are not isolated from each other by hybrid inviability nor by hybrid sterility, although the hybrids showed somewhat abnormal spermatogenesis in hybrids and that H. rugulosus is isolated from both H. litoralis and H. tigerinus by incomplete hybrid inviability and complete hybrid sterility.

参考文献/References:

Alam M. S., Islam M. M., Khan M. M. R., Hasan M., Wanichanon R., Sumida M. 2012. Postmating isolation in six species of three genera (Hoplobatrachus, Euphlyctis and Fejervarya) from family Dicroglossidae (Anura), with special reference to spontaneous production of allotriploids. Zool Sci, 29: 743–752
Coyne J.A., Orr H. A. 1989. Patterns of speciation in Drosophila. Evolution, 43: 362–381
de Queiroz K. 1998. The general lineage concept of species, species criteria, and the process of speciation: A conceptual unification and terminological recommendations. In Howard D.J., Berlocher S. H., (Eds.), Endless forms: Species and speciation. New York, NY, USA: Oxford University Press, 57–75
Dobzhansky T. 1937. Genetics and the origin of species. New York: Columbia University Press
Dobzhansky T. 1950. Mendelian populations and their evolution. Am Nat, 84: 401–418
Ferguson J. W. H. 2002. On the use of genetic divergence for identifying species. Biol J Linn Soc, 75: 509–516
Frost D. R. 2016. Amphibian species of the world: an online reference. Version 6.0. Electronic Database accessible at http://research.amnh.org/vz/herpetology/amphibia/
New York: American Museum of Natural History (accessed 30 March 2016)
Futuyma D. J. 2005. Evolution. Sunderland, MA, USA: Sinauer Associates
Hasan M., Islam M. M., Khan M. M. R., Alam M. S., Kurabayashi A., Igawa T., Kuaramoto M., Sumida M. 2012a. A new species of genus Hoplobatrachus (Anura, Dicroglossidae) from the coastal belt of Bangladesh. Zootaxa, 3312: 45–58
Hasan M., Islam M. M., Khan M. M. R., Alam M. S., Kurabayashi A., Igawa T., Kuaramoto M., Sumida M. 2012b. Cryptic anuran biodiversity in Bangladesh revealed by mitochondrial 16S rRNA gene sequences. Zool Sci, 29: 162–172
Hasan M., Islam M. M., Kuramoto M., Kurabayashi A., Sumida M. 2014. Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh. Zootaxa, 3755: 401–418
Hoskin C. J., Higgie M., McDonald K. R., Moritz C. 2005. Reinforcement drives rapid allopatric speciation. Nature, 437: 1353–1356
Mallet J. 1998. Isolating mechanisms. In Calow P. (Ed), Encyclopaedia of ecology and environmental management. Blackwell Press, 379–380
Matsui M. 2011. On the brown frogs from the Ryukyu Archipelago, Japan, with description of two new species (Amphibia, Anura). Curr Herpetol, 30: 111–128
Mayden R. L. 1997. A hierarchy of species concepts: the denouement in the saga of the species problem. In Claridge M.F., Dawah H.A., Wilson M.R. (Eds.), Species: The units of biodiversity. London, UK: Chapman and Hall, 381–424
Mayden R. L. 1999. Consilience and a hierarchy of species concepts: advances toward closure on the species puzzle. J Nematol, 31: 95–116
Mayr E. 1942. Systematics and the origin of species. New York: Columbia University Press
Mayr E. 1963. Animal species and evolution. Cambridge, MA: Belknap Press
Mayr E. 1969. The biological meaning of species. Biol J Linn Soc, 1:311–320
Mayr E. 1970. Populations, species, and evolution. Cambridge, MA: Harvard University Press
Mayr E. 1982. The growth of biological thought: Diversity, evolution, and inheritance Cambridge, MA, USA and London, UK: Belknap Press of Harvard University Press
Sasa M. M., Chippindale P. T., Johnson N. A. 1998. Patters of postzygotic isolation in frogs. Evolution, 53: 1811–1820
Schmid M., Olert J., Klett C. 1979. Chromosome banding in Amphibia. ΙΙΙ. Sex chromosomes in Triturus. Chromosoma, 71: 29–55
Sites J. W., Marshall J. C. 2003. Delimitation species: a renaissance issue in systematic biology. Trends Ecol Evol, 18: 462–470
Sites J. W., Marshall J. C. 2004. Operational criteria for delimiting species. Annu Rev Ecol Evol Syst, 35: 199–227
Sultana N., Igawa T., Islam M. M., Hasan M., Alam M. S., Komaki S., Kawamura K., Khan M. M. R., Sumida M. 2016. Inter-and intra- specific genetic divergence of Asian tiger frogs (genus Hoplobatrachus), with special reference to the population structure of H. tigerinus in Bangladesh. Genes Genet Syst, in press
Sumida M., Kotaki M., Islam M. M., Djong T. H., Igawa T., Kondo Y., Matsui M., Anslem de S., Khonsue W., Nishioka M. 2007. Evolutionary relationships and reproductive isolating mechanisms in the rice frog (Fejervarya limnocharis) species complex from Sri Lanka, Thailand, Taiwan and Japan, inferred from mtDNA gene sequences, allozymes, and crossing experiments. Zool Sci, 24: 547–562
Sumida M., Ueda H., Nishioka M. 2003. Reproductive isolating mechanisms and molecular phylogenetic relationships among Palearctic and Oriental brown frogs. Zool Sci, 20: 567–580
Sumida M., Satou N., Yoshikawa N., Kurabayashi A., Islam M. M., Igawa T., Oumi S., Katsuren S., Ota H., Shintani N., Fukuniwa H., Sano N., Fuji T. 2011. Artificial production and natural breeding of the endangered frog species Odorrana ishikawae, with special reference to fauna conservation in the laboratory. Zool Sci, 28: 834–839
Tilley S. G., Verrel P. A., Arnod S. J. 1990. Correspondence between sexual isolation and allozyme differentiation: A test in salamander (Desmognathus ochrophaeus). Proc Natl Acad Sci USA, 87: 2715–2719
Turelli M., Barton N. H., Coyne J. A. 2001. Theory and speciation. Trends Ecol Evol, 16: 330–343
Wright S. 1940. The statistical consequences of Mendelian heredity in relation to speciation. In Huxley J. (Ed.), The new systematics. London, UK: Oxford University Press, 161–183
Wu C. I., Holloncher H. 1998. Subtle is nature: the genetics of species differentiation and speciation. In Howard D. J., Brocher S. H., (Eds.), Endless forms: Species and speciation. New York: Oxford University Press

更新日期/Last Update: 2017-03-25