[1].Hematology of Wild Caught Hoplobatrachus rugulosus in Northern Thailand[J].Asian Herpetological Research,2016,7(2):131-138.[doi:10.16373/j.cnki.ahr.150037]
 Suthirote MEESAWAT,Noppadon KITANA,and Jirarach KITANA,et al.Hematology of Wild Caught Hoplobatrachus rugulosus in Northern Thailand[J].Asian Herpetological Reserch(AHR),2016,7(2):131-138.[doi:10.16373/j.cnki.ahr.150037]

Hematology of Wild Caught Hoplobatrachus rugulosus in Northern Thailand()

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]



Hematology of Wild Caught Hoplobatrachus rugulosus in Northern Thailand
Suthirote MEESAWAT1 Noppadon KITANA1 2 and Jirarach KITANA1 2*
1 Department of Biology, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
2 Center of Excellence in Biodiversity, Faculty of Science, Chulalongkorn University, Bangkok 10330, Thailand
Rice field frog erythrocyte leukocyte packed cell volume morphometry Nan Province
The rice field frog Hoplobatrachus rugulosus is an important anuran species found in wetlands throughoutThailand. At present, the hematological parameters of wild populations are unknown. Therefore, hematological andmorphological characteristics of peripheral blood cells of wild-caught H. rugulosus were examined. Thirty-three adultfrogs (17 male and 16 female frogs) were collected from a natural population in Nan Province, northern Thailandduring the wet season of 2014. Blood samples were analyzed by packed cell volume (PCV) and blood cell counts fromhemocytometer and Giemsa-stained blood smears. The mean PCV of male frogs (30.70% ± 6.07%) was significantlyhigher than that of the female frogs (25.09% ± 4.85%). The mean number of lymphocytes and neutrophils also showedsignificant sex-related differences. Moreover, the morphometric analysis of blood cells revealed dimensions as follows:erythrocytes (17.96 ± 1.44 μm length × 11.50 ± 1.09 μm width), immature erythrocytes (14.91 ± 2.20 μm diameter),thrombocytes (13.93 ± 3.14 μm length × 7.05 ± 1.31 μm width), lymphocytes (11.01 ± 2.69 μm diameter), monocytes(12.04 ± 2.40 μm diameter), neutrophils (12.58 ± 2.08 μm diameter), basophils (13.60 ± 2.17 μm diameter) andeosinophils (12.33 ± 2.95 μm diameter). Overall, the hematological parameters obtained in this study could be regardedas the first report and a crucial baseline data of wild H. rugulosus in Thailand that can be used for monitoring the healthstatus of this anuran.


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更新日期/Last Update: 2016-06-25