[1].Effects of Pesticide Exposure on Embryonic Development and Hatchling Traits of Turtles[J].Asian Herpetological Research,2016,7(1):28-33.[doi:10.16373/j.cnki.ahr.150024]
 Baofeng WU,Liang LIANG,Liang MA and Weiguo DU*.Effects of Pesticide Exposure on Embryonic Development and Hatchling Traits of Turtles[J].Asian Herpetological Reserch(AHR),2016,7(1):28-33.[doi:10.16373/j.cnki.ahr.150024]

Effects of Pesticide Exposure on Embryonic Development and Hatchling Traits of Turtles()

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]



Effects of Pesticide Exposure on Embryonic Development and Hatchling Traits of Turtles
Baofeng WU Liang LIANG Liang MA and Weiguo DU*
Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, People’s Republic of China
Parchment-shelled eggs Rigid-shelled eggs Red-eared slider turtle Trachemys scripta Chinese three-keeled pond turtle Chinemys reevesii Embryo Environmental hormone
Deltamethrin is a widespread environmental hormone with endocrine-disrupting properties, but its effect on embryonic development of reptiles is largely unexplored. We investigated the effects of deltamethrin on embryonic development and offspring traits in two turtle species, one with parchment-shelled eggs and the other with rigid-shelled eggs. Deltamethrin exposure during egg incubation did not affect hatching success and hatchling body size in either species. However, embryonic exposure to deltamethrin resulted in reduced hatchling locomotor performance in the red-eared slider turtle (Trachemys scripta) with parchment-shelled eggs, but not in the Chinese three-keeled pond turtle (Chinemys reevesii) with rigid-shelled eggs. These results suggest that parchment-shelled eggs are likely more vulnerable to deltamethrin than rigid-shelled eggs.


Amweg E. L., Weston D. P., Ureda N. M. 2005. Use and toxicity of pyrethroid pesticides in the Central Valley, California, USA. Environ Toxicol Chem, 24: 966–972
Aziz M. H., Agrawal A. K., Adhami V. M., Shukla Y., Seth P. K. 2001. Neurodevelopmental consequences of gestational exposure (GD14-GD20) to low dose deltamethrin in rats. Neurosci Lett, 300: 161–165
Belinsky A., Ackerman R. A., Dmi’el R., Ar A. 2004. Water in reptilian eggs and hatchlings. In: Deeming D. C., editor. Reptilian incubation: environment, evolution and behaviour. Nottingham, UK: Nottingham University Press. pp. 125–41
Bishop C. A, Ng P., Pettit K. E., Kennedy S., Stegerman J. J., Norstrom R. J., Brooks R. J. 1998. Environmental contamination and developmental abnormalities in eggs and hatchlings of the common snapping turtle (Chelydra serpentina serpentina) from the Great Lakes–St Lawrence River basin (1989-1991). Environ Pollut, 99: 1–14
Bridges C. M. 2000. Long-term effects of pesticide exposure at various life stages of the southern leopard frog (Rana sphenocephala). Arch Environ Con Tox, 39: 91–96
Coeurdassier M., De Vaufleury A., Badot P. M. 2003. Bioconcentration of cadmium and toxic effects on life-history traits of pond snails (Lymnaea palustris and Lymnaea stagnalis) in laboratory bioassays. Arch Environ Con Tox, 45: 102–109
Crews D., Bergeron J. M. 1994. Role of reductase and aromatase in sex determination in the red-eared slider turtle, a turtle with temperature-dependent sex determination. J Endocrinol, 143: 279–289
Crews D., Bull J. J., Wibbels T. 1991. Estrogen and sex reversal in turtles: A dose-dependent phenomenon. Gen Comp Endocr, 81: 357-364
Datta M., Kaviraj A. 2003. Acute toxicity of the synthetic pyrethroid deltamethrin to freshwater catfish Clarias gariepinus. B Erviron Contam Tox, 70: 296–299
Deeming D. C., Thompson M. B. 1991. Gas exchange across reptilian eggs. In: Deeming D. C., Ferguson M. W. J. (Eds). Egg incubation: its effects on embryonic development in birds and reptiles. Cambridge, UK: Cambridge University Press. pp 277–284
Deeming D. C., Unwin D. M. 2004. Reptilian incubation: evolution and the fossil record. In: Deeming D. C., editors. Reptilian incubation: environment, evolution and behaviour. Nottingham University Press, Nottingham, pp. 1–14
Delistraty D. 2000. Acute toxicity to rats and trout with a focus on inhalation and aquatic exposures. Ecotox Environ Safe, 46: 225–233
de Solla S. R., Martin P. A., Mikoda P. 2011. Toxicity of pesticide and fertilizer mixtures simulating corn production to eggs of snapping turtles (Chelydra serpentina).?Sci Total Environ,?409: 4306–4311
Du W. G., Hu L. J., Lu J. L., Zhu L. G. 2007. Effects of incubation temperature on embryonic development rate, sex ratio and post-hatching growth in the Chinese three-keeled pond turtle, Chinemys reevesii. Aquaculture, 272:747–753
Du W. G., Shine R. 2015. The behavioural and physiological strategies of bird and reptile embryos in response to unpredictable variation in nest temperature. Biol Rev, 90: 19–30
Greulich K., Pfluqmacher S. 2003. Differences in susceptibility of various life stages of amphibians to pesticide exposure. Aquat toxicol, 65: 329–336
Hopkins W. A., Winne C. T. 2006. Influence of body size on swimming performance of four species of neonatal natricine snakes acutely exposed to a cholinesterase-inhibiting pesticide. Environ Toxicol Chem, 25: 1208–1213
Kenan K., Rahmi A. 2004. The toxic effects of pyrethroid deltamethrin on the common carp (Cyprinus carpio L.) embryos and larvae. Pestic Biochem Phys, 80: 47–53
Kontreczky C., Farkas A., Nemcsok J., Salanki J. 1997. Short- and long-term effects of deltamethrin on filtering activity of freshwater mussel (Anodonta cygnea L.). Ecotox Environ Safe, 38: 195–199
Kusuda S., Yasukawa Y., Shibata H., Saito T., Doi O., Ohya Y. Yoshizaki N. 2013. Diversity in the matrix structure of eggshells in the Testudines (Reptilia). Zool Sci, 30: 366–374
Lazarini C. A., Florio J. C., Lemonica I. P., Bernardi M. M. 2001. Effects of prenatal exposure to deltamethrin on forced swimming behavior, motor activity, and striatal dopamine levels in male and female rats. Neurotoxicol Teratol, 23: 665–673
McKinlay R., Plant J. A., Bell J. N., Voulvoulis N. 2008. Endocrine disrupting pesticides: implications for risk assessment. Environ Int, 34: 168–183
Moretti M., Villarini M., Scassellati-Sforzolini G., Pasquini R., Monarca S. 1997. Applicability of aspecific noninvasive methods for biomonitoring of occupational exposure to deltamethrin: preliminary study using an animal model. Arch Environ Con Tox, 33: 323–328
Mubarak H. M., Suzuki T., Sato N., Sato I., Takewaki T., Suzuki K., Tachikawa E., Kobayashi H. 2006. Differential effects of pyrethroid insecticides on extracellular dopamine in the striatum of freely moving rats. Toxicol Appl Pharm, 217: 25–34
Oftedal O. T. 2002. The origin of lactation as a water source for parchment-shelled eggs. J. Mammary Gland Biol, 7: 253–266
Oros D. R., Werner I. 2005. Pyrethroid insecticides: an analysis of use patterns, distributions, potential toxicity and fate in the Sacramento-San Joaquin delta and central valley. White paper for the Interagency Ecological Program. SEFI Contribution 415. San Francisco Estuary Institute, Oakland, CA
Pawlisz J., Busnarda J., McLauchlin A., Caux P. Y., Kent R. A. 1998. Canadian water quality guidelines for deltamethrin. Environ Toxicol Water Qual, 13: 175–210
Podreka S., Georges A., Maher B., Limpus C. J. 1998. The environmental contaminant DDE fails to influence the outcome of sexual differentiation in the marine turtle Chelonia mydas. Environ Health Persp, 106: 185–188
Presibella K. M., Kita D. H., Carneiro C. B., Andrade A. J., Dalsenter P. R. 2005. Reproductive evaluation of two pesticides combined (deltamethrin and endosulfan) in female rats. Reprod Toxicol, 20: 95–101
Puglis H. J., Boone M. D. 2007. Effects of a fertilizer, an insecticide, and a pathogenic fungus on hatching and survival of bullfrog (Rana catesbeiana) tadpoles. Environ Toxicol and Chem, 26: 2198–2201
Righi D. A., Xavier F. G., Palermo N. J. 2009. Effects of type II pyrethroid cyhalothrin on rat innate immunity: a flow cytometric study. Int Immunopharmacol, 9: 148–152
Santos D., Matos M., Coimbra A. M. 2014. Developmental toxicity of endocrine disruptors in early life stages of zebrafish, a genetic and embryogenesis study. Neurotoxicol Teratol, 46: 18–25
Schirling M., Bohlen A., Triebskorn R., Koehler H. R. 2006. An invertebrate embryo test with the apple snail Marisa cornuarietis to assess effects of potential developmental and endocrine disruptors. Chemosphere, 64: 1730–1738
Sinha C. Agrawal A. K., Islam F., Seth K., Chaturvedi R. K., Shukla S., Seth P. K. 2004. Mosquito repellent (pyrethroid-based) induced dysfunction of blood-brain barrier permeability in developing brain. Int J Dev Neurosci, 22: 31–37
Solla S. R. D., Martin P. A., Mikoda P. 2011. Toxicity of pesticide and fertilizer mixtures simulating corn production to eggs of snapping turtles (Chelydra serpentina). Sci Total Environ, 409: 4306–4311
Solla, S. R. D., Palonen, K. E., Martin, P. A. 2014. Toxicity of pesticides associated with potato production, including soil fumigants, to snapping turtle eggs (Chelydra serpentina). Environ Toxicol Chem, 33: 102–106
Sparling D. W., Matson C., Bickham J., Doelling-Brown P. 2006. Toxicity of glyphosate as Glypro an LI700 to red-eared slider (Trachemys scripta elegans) embryos and early hatchlings. Environ Toxicol Chem, 25: 2768–2774
Svobodova Z., Luskova V., Drastichova M. J., Svoboda M., Zlabek V. 2003. Effect of deltamethrin on haematological indices of common carp (Cyprinus carpio L.). Acta Vet Brno, 72: 79–85
Thybaud E. 1990. Toxicité aigu? et bioconcentration du lindane et de la deltaméthrine par les têtards de Rana temporaria et les gambusies (Gambusia affinis). Hydrobiologia, 190: 137–145
Tidou A. S., Moreteau J. C., Ramade F. 1992. Effects of lindane and deltamethrin on zooplankton communities of experimental ponds. Hydrobiologia, 232: 157–168
U.S. Department of Agriculture. Agricultural Resource Management Survey Tool. 2007. http://www.ers.usda.gov/Data/ARMS/app/Crop.aspx
Wibbels T, Bull J. J., Crews D. 1994. Temperature-dependent sex determination: a mechanistic approach. J Exp Zool, 270: 71–78
Willingham E. J. 2005. The effects of atrazine and temperature on turtle hatchling size and sex ratios. Front. Ecol. Environ. 3: 309–313
Yntema C. L. 1976. Effects of incubation temperatures on sexual differentiation in the turtle, Chelydra serpentina. J Morphol, 150: 453-461
Zar J. H. 1999. Biostatistical analysis. Prentice Hall, Upper Saddle River, N. J.

更新日期/Last Update: 2016-03-30