[1].Structure and Function of the Gastrointestinal Tract of the Green Turtle (Chelonia mydas) Hatchling[J].Asian Herpetological Research,2015,6(4):317-330.[doi:10.16373/j.cnki.ahr.140074]
 Hualing CHEN*,Mingbin YE*,Yuyan LU,et al.Structure and Function of the Gastrointestinal Tract of the Green Turtle (Chelonia mydas) Hatchling[J].Asian Herpetological Reserch(AHR),2015,6(4):317-330.[doi:10.16373/j.cnki.ahr.140074]

Structure and Function of the Gastrointestinal Tract of the Green Turtle (Chelonia mydas) Hatchling()

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]



Structure and Function of the Gastrointestinal Tract of the Green Turtle (Chelonia mydas) Hatchling
Hualing CHEN12* Mingbin YE2* Yuyan LU1 Jinxia DUAN2 Pipeng LI1** and Hexiang GU2**
1 Institute of Herpetology and Liaoning Key Lab of Evolution and Biodiversity, Shenyang Normal University, Shenyang 110034, China
2 National Huidong Sea Turtle Nature Reserve Management Bureau, Huidong 516359, Guangdong, China
anatomy histology ultrastructure digestive tract diet sea turtle
The gastrointestinal tracts of four Chelonia mydas hatchlings were examined at the anatomical, histological and ultrastructural level. Our results show that the gastrointestinal tract (GI) is composed by esophagus, stomach, small intestine (SI) and large intestine (LI), and histologically of mucosa, submucosa, muscularis externa (ME) and serosa. The esophagus is marked by conical papillae lined by keratinized stratified squamous epithelium, whereas the remaining GI by simple columnar epithelium; esophageal diverticulum is absent. The stomach covered with mucous granule cells, contains cardia, fundic regions and pylorus, which are separately characterized by cardiac glands, fundic glands and pyloric glands, and have the thickest submucosa and ME of the GI. The ME of the esophagus mainly consist of one layer of circular smooth muscle whereas the rest of GI of two layers, inner circular muscle and outer longitudinal muscle. The SI is slightly longer than the LI and the GI is approximately 5.11 times of the carapace length. The SI is lined with longitudinal zigzag folds and characterized by absorptive cells with longer and denser microvilli, whereas the LI by transversal folds, goblet cells and lymphoid nodules. Only intestinal glands appear in duodenum. Endocrine cells are observed in all sections of the GI and accounted for the largest proportion in duodenum. The results demonstrate a perfect combination of the structure and function of the GI and reveal that the digestion and absorption primarily occurs in the foregut. C. mydas hatchling may prefer carnivorous diet.


Arthur K. E., Boyle M. C., Limpus C. J. 2008. Ontogenetic changes in diet and habitat use in green sea turtle (Chelonia mydas) life history. Mar Ecol Prog Ser, 362: 303–311
Bagchi A., Herrup E. A., Warren H. S., Trigilio J., Shin H. S., Valentine C., Hellman J. 2007. MyD88-dependent and MyD88-independent pathways in synergy, priming, and tolerance between TLR agonists. J Immunol, 178: 1164–1171
Balazs G. H., Murakawa S. K. K., Wyneken J., Schroeder B. A. 1998. Differences in Flipper Size and Esophagus Morphology of Green Turtle from Hawaii and Florida. U.S. Dep. Commer.: NOAA Tech. Memo. NMFS-SEFSC-415, 137–138 pp
Bell F. R. 1979. The relevance of the new knowledge of gastrointestinal hormones to veterinary science. Vet Res Commun, 2(1): 305–314
Bjorndal K. A. 1979. Cellulose digestion and volatile fatty acid production in the green turtle, Chelonia mydas. Comp Biochem Physiol, 63(1): 127–133
Bjorndal K. A., Suganuma H., Bolten A. B. 1997. Foraging ecology and nutrition of sea turtles. In: Lutz P., Musick J. (Eds.), The Biology of Sea Turtles. Boca Raton: CRC Press, 199–232
Bleakney J. S. 1965. Reports of marine turtles from New England and Eastern Canada. Can Field Nat, 79(2): 120–128
Brand-Gardner S. J., Lanyon J. M., Limpus C. J. 1999. Diet selection by immature green turtles, Chelonia mydas, in subtropical Moreton Bay, south-east Queensland. Aust J Zool 47(2): 181–191
Carr A. 1987. New perspectives on the pelagic stage of sea turtle development. Conserv Biol, 1(2): 103–121
Cheng L., Zhong C., Cai W. 2003. Contemporary Histology. China: Shanghai Scientific and Technological Literature Publishing House, 786–828 pp
Chen H.L., Ye M.B., Lin R.J. 2006. High density feeding of green turtle through Hibernation. Sichuan J Zool, 25(2): 395–397
Coutinho H. B., Carmona da mota H., Coutinho V. B., Robalinho T. I., Furtado A. F., Walker E., King G., Mahida Y. R., Sewell H. F., Wakelin D. 1998. Absence of lysozyme (muramidase) in the intestinal Paneth cells of newborn infants with necrotising enterocolitis. J Clin Pathol, 51(7): 512–514
Crane R. K. 1960. Intestinal absorption of sugars. Physiol Rev, 40: 789–825
Dijk P. P., Iverson J. B., Shaffer H. B., Bour R., Rhodin A.G.J. 2011.Turtles of the world, 2011 update:?annotated checklist of taxonomy, synonymy, distribution, and conservation status. Chelonian Res Monogr, 4(5):165–242
Ernst C. H., Barbour R. W., Lovich J. E. 1994.Turtles of the United States and Canada. Washington [u.a.]: Smithsonian Inst Press
Fahrenholz C. 1937. Drusen der Mundhohle, In: Bolk L., Goppert E., Kallius E., Lubosch W.(Eds.), Handbuch der vergleichenden Anatomie der Wirbeltiere. Vienna: Urban u. Schwarzen-berg, 115–155pp
Fu L.R., Hong M.L., Shi H.T., Wang L.J., Huang Y.H. 2007. Preliminary study on histological structure of digestive system of Ocadia sinensis. Shichuan J Zool, 26(2): 269–274
Fosdick P., Fosdick S. 1994. Last Chance Lost? Can and Should Farming Save the Green Sea Turtle? The Story of Mariculture Ltd.-Cayman Turtle Farm. New York, Pennsylvania: Irvin S. Naylor, 1–50pp
Gan W., Zhang X., Qiao J. 2011. Histological observation of the digestive track of green turtle (Chelonia mydas). Chin J Vet Med, 47(11): 25–27
Gen?er Tarak?i B., Fmfiek K?prücü S., Yaman M. 2005. An immunohistochemical study on the endocrine cells in the gastrointestinal tract of the freshwater turtle, Mauremys caspica caspica. Turk J Vet Anim Sci, 29: 581–587
Gore R. M., Levine M. S. 2007. Textbook of Gastrointestinal Radiology. Philadelphia, PA.: Saunders
Gray H., Lewis W. H. 2000. Gray’s Anatomy of the Human Body. 20th Ed. New York, NY: Bartleby
Hildebrand M., Goslow G.E. 2006. Análise da Estrutura dos Vertebrados. S?o Paulo, Atheneu, 637p
Iwasaki S. I., Yoshizawa H., Kawahara I. 1996. Three-dimensional ultrastructure of the surface of the tongue of the rat snake, Elaphe climacophora. Anat Rec, 245: 9–12
Junqueira L. C., Carneiro, J. 1999. Histologia Ba?sica. 9a ed. Rio de Janeiro: Guanabara Koogan
Kochva E. 1978. Oral glands of the reptilia. In: Gans C. (Eds), Biology of the Reptilia. London: Academic Press, 43–161
Latunde-Dada G. O., Van Der Westhuizen J., Vulpe C. D., Anderson G. J., Simpson R. J., Mckie A. T. 2002. Molecular and functional roles of duodenal cytochrome B (Dcytb) in iron metabolism. Blood Cells Mol Dis, 29(3): 356–360
Li G. S., Tang F. X. 2014. Observations on the mucosa of digestive tract in two turtles by SEM. J Jinan Univ, 35(4): 344–349
Magalh?es M. D. S., Santos A. J. B., Silva N. B. D., Moura C. E. B. D. 2012. Anatomy of the digestive tube of sea turtles (Reptilia: Testudines). Zoologia, 29(1): 70–76
Magalh?es M. S., Freitas M. D. L., Silvae N. B. D., Moura C. E. B. D. 2010. Morfologia do tubo digestório da tartaruga verde (Chelonia mydas). Pesquisa Veterinária Brasileira, 30(8): 676–684
Magalh?es M. S., Vogt R. C., Barcellos J. F. M., Moura C. E. B., Silveira R. D. 2014. Morphology of the digestive tube of the Podocnemididae in the Brazilian Amazon. Herpetologica, 70(4): 449–463
Mrosovsky N., Ryan G. D., James, M. C. 2009. Leatherback turtles: The meance of plastic. Mar Pollut Bull, 58(2): 287–289
Marieb E. M. 1995. Human Anatomy and Physiology (3rd ed.). Redwood City, US: Benjamin/Cummings, 103–104 pp
Melo C. M. F., Batista D. P., Amora T. D., Guarana P. T. M., Andrade M. B., Santos R. M. B., Miglino M. A. 2008. Comparative anatomy study of the stomachs between green turtle (Chelonia mydas) and leatherback turtle (Dermochelys coriacea). Braz J Morphol Sci, 25(1–4): 121–138
Morton B., Morton J. E.1983.The sea shore ecology of Hong Kong. J Anim Ecol,1–350?
Nagaoka S., Martins A., Santos R., Tognella M., Filho E. O., Seminoff J. 2012. Diet of juvenile green turtles (Chelonia mydas) associating with artisanal ?shing traps in a subtropical estuary in Brazil. Mar Biol, 159: 573–581
Ovaere P., Lippens S., Vandenabeele P., Declercq W. 2009. The emerging roles of serine protease cascades in the epidermis. TiBS, 34(9): 453–463
Pearse A. G. E. 1968. Histochemistry, Theoretical and Applied. London: Churchill Livingstone, 530 pp
Peng Y., Yang J. 2011. Re-improvements of Gill’s hematoxylin dyeing reagent. World Health Digest Medical Periodieal, 8(43): 257–258
Rainey W. E. 1981. Guide to Sea Turtle Visceral Anatomy. University of California, Berkeley: NOAA Tech. Memo. NMFS-SEFC, 80 pp
Reich K. J., Bjorndal K. A., Bolten A. B. 2007. The ’lost years’ of green turtles: using stable isotopes to study cryptic lifestages. Biol letters, 3(6): 712–714
Reynolds J. E., Rommel S. A. 1996. Structure and function of the gastrointestinal tract of the Florida Manatee, Trichechus manatus latirostris. Annto Rec, 245(3): 539–558
Romer A. S., Parsons T. S. 1977. The Vertebrate Body. Philadelphia, PA: Holt-Saunders International, 349–353 pp
Romer A. S., Parsons T. S. 1995. Anatomia comparada dos vertebrados. Atheneu, Sa?o Paulo, 559 pp
Stevens C. E., Hume I. D. 1998. Contributions of microbes in vertebrate gastrointestinal tract to production and conservation of nutrients. Physiol Rev, 78(2): 393–427
Sabyasachi S. 2008. Principles of Medical Physiology. Thieme Medical Pub. 435 pp
Silva N. B. 2005. Histologia do sistema digesto?rio de sagu?iru, Steindachnerina notonota (Miranda Ribeiro 1937). Boletim do Instituto de Pesca, 31(1): 1–8
Thompson S. M. 1980. A Comparative Study of the Anatomy and Histology of the Oral Cavity and Alimentary Canal of Two Sea Turtles the Herbivorous Green Turtle Chelonia mydas and the Carnivorous Loggerhead Turtle Caretta caretta. M.S. Thesis. James Cook University. 313 pp
Wyneken J. 2001. The Anatomy of Sea Turtles. U.S. Department of Commerce NOAA Tech. Memo. NMFS-SEFSC-470
Wang W., Du K. H.1996. A histological study on the digestive system in the Trionyx sinensis wiegmann. J Nanjing Norm Univ, 19(2): 52–56
Wu X. B., Zhang S. Z., Chen B. H., Wang C. L., Xie W. S. 2001.Histochemical and ultrastructural studies on the stomach of Alligator sinesis. ACTA Hydrobiol Sin, 25(3): 289–293
Work T. M. 2000. Manual de Necropsia de T ortugas Marinas para Biologos en Refugios o Areas Remotas. Hawaii, U.S. Geological Survey National Wildlife Health Center, Hawaii Field Station, 25p
Xu S.K., Liu Z.X. 1996. Anatomy of the digestive system and the respiratory system of Testudo horsfieldl. Chin J Zool, 31(3): 36–39
Ye M. B., Xia Z. R., Chen H. L. 2012. Anatomy of several systems in Olive Ridley sea turtle. J Snake, 24(3): 237–240
Young B., Lowe J. S., Stevens A., Heath J. W., Deakin P. J. 2006. Wheater’s Functional Histology: A Text and Volour Atlas (5th ed. ed.). Edinburgh: Churchill Livingstone/Elsevier, 273 pp
Zhang F.Y., Gu H.X. 2005. Artificial feeding of baby turtle of Chelonia mydas. Sichuan J Zool, 24(3): 412–413

更新日期/Last Update: 2016-01-25