[1].Comparative Studies on Sperm Ultrastructure of Three Gecko Species, Gekko japonicus, Gekko chinensis and Hemidactylusbowrigii (Reptilia, Squamata, Gekkonidae)[J].Asian Herpetological Research,2015,6(3):189-198.[doi:10.16373/j.cnki.ahr.140071]
 Shuangli HAO,Liangliang PAN,Zhouxi FANG and Yongpu ZHANG*.Comparative Studies on Sperm Ultrastructure of Three Gecko Species, Gekko japonicus, Gekko chinensis and Hemidactylusbowrigii (Reptilia, Squamata, Gekkonidae)[J].Asian Herpetological Reserch(AHR),2015,6(3):189-198.[doi:10.16373/j.cnki.ahr.140071]
点击复制

Comparative Studies on Sperm Ultrastructure of Three Gecko Species, Gekko japonicus, Gekko chinensis and Hemidactylusbowrigii (Reptilia, Squamata, Gekkonidae)()
分享到:

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

卷:
6
期数:
2015年3期
页码:
189-198
栏目:
出版日期:
2015-09-25

文章信息/Info

Title:
Comparative Studies on Sperm Ultrastructure of Three Gecko Species, Gekko japonicus, Gekko chinensis and Hemidactylusbowrigii (Reptilia, Squamata, Gekkonidae)
文章编号:
AHR-2014-0071
Author(s):
Shuangli HAO1 Liangliang PAN2 Zhouxi FANG2 and Yongpu ZHANG1*
1 College of Life and Environmental Science, Wenzhou University, Wenzhou 325035, China
2 College of Life Science, Wenzhou Medical University, Wenzhou 325035, China
Keywords:
Spermatozoa ultrastructure Gekkonidae phylogenetic relationship
DOI:
10.16373/j.cnki.ahr.140071
Abstract:
We provide the first description of the ultrastructure of the spermatozoa of Gekko japonicus, Gekko chinensis and Hemidactylus bowrigii for further understanding of the phylogenetic relationships of Gekkonidae. Mature spermatozoa of the three species differ in the occurrence and shape of epinuclear electron-lucent zone, nuclear space, neck cylinder and mitochondria. G. japonicus and G. chinensis have similar spermatozoan ultrastructure while H. bowrigii differs from these two species. In addition, these three species have neck cylinder with mitochondria in neck region and dense bodies arranged in grid with mitochondria in the midpiece, which may be the autapomorphies of the family Gekkonidae. Statistical analyses reveal that: total length of the spermatozoa was significantly different between G. japonicus and G. chinensis, as well as between G. japonicus and H. bowrigii (F2,57 = 23.66, P<0.0001); G. japonicus and H. bowrigii differ in head length (F2,43 = 4.64, P<0.05) and the width of nuclear base (F2,22 = 3.97, P<0.05). In addition, the midpiece length (F2,33 = 23.66, P<0.01) of the spermatozoa was significantly different between H. bowrigii and G. japonicus, and also between H. bowrigii and G. chinensis. Lengths of perforatorium, acrosomal complex and nuclear rostrum and the width of nuclear shoulder are similar in all three species. Our results indicated that the sperm ultrastructure contained intra and intergeneric variabilities which is helpful for better understanding their genetic relationships.

参考文献/References:

Cunha L. D., Tavares-Bastos L., Báo S. N. 2008. Ultrastructural description and cytochemical study of the spermatozoon of Crotallus durissus (Squamata, Serpentes). Micron, 39: 915–925
Colli G. R., Teixeira R. D., Scheltinga D. M., Mesquita D. O., Wiederhecker H. C., Báo S. N. 2007. Comparative study of sperm ultrastructure of five species of teiid lizards (Teiidae, Squamata), and Cercosaura ocellata (Gymnophthalmidae, Squamata). Tissue Cell, 39: 59–78
Furieri P. 1970. Sperm morphology of some reptiles: Squamata and Chelonia. In Baccetti B. (Ed.), Comparative Spermatology. Rome: Accademia Nazionale dei Lincei, 115–131
Giugliano L. G., Teixeira R. D., Colli G. R., Báo S. N. 2002. Ultrastructure of spermatoxoa of the Lizard Ameiva ameiva, with considerations on polymorphism within the Family Teiidae (Squamata). J Morph, 253: 264–271
Hu J. R., Liu M., Wang D. H., Hu Y. J, Tan F. Q., Yang W. X. 2013. Molecular characterization and expression analysis of a KIFC1-like kinesin gene in the testis of Eumeces chinensis. Mol Biol Rep, 40: 6645–6655
Hu J. R., Xu N., Tan F. Q., Wang D. H., Liu M., Yang W. X. 2012. Molecular characterization of a KIF3A-like kinesin gene in the testis of the Chinese fire-bellied newt Cynops orientalis. Mol Biol Rep, 39(4): 4207–4214
Hou C. C., Yang W. X. 2013. Acroframosome-dependent KIFC1 facilitates acrosome formation during spermatogenesis in the caridean shrimp Exopalaemon modestus. PloS ONE, 8(9): 1–16
Han D. M., Zhou K. Y., Bauer A. M. 2004. Phylogenetic relationships among gekkotan lizards inferred from C-mos nuclear DNA sequences and a new classification of the Gekkota. Biol J Linn Soc, 83: 353–368
Jamieson B. G. M. 1995. The ultrastructure of spermatozoa of the Squamata (Reptilia) with phylogenetic considerations. In Jamieson B. G. M., Ausio J., Justine J. (Eds.), Advances in Spermatozoal Phylogeny and Taxonomy. Paris: Muséum National d’Histoire Naturelle, 359–383
Jamieson B. G. M., Healy J. M. 1992. The phylogenetic position of the tuatara Sphenodon (Sphenodontida, Amniota), as indicated by cladistic analysis of the ultrastructure of spermatozoa. Philos Trans R Soc London (B), 335: 207–219
Jamieson B. G. M., Koehler L. 1994. The ultrastructure of the spermatozoon of the northern water snake, Nerodia sipedon (Colubridae, Serpentes), with phylogenetic considerations. Can J Zool, 72: 1648–1652
Jamieson B. G. M., Oliver S. C., Scheltinga D. M. 1996. The ultrastructure of spermatozoa of Squamata, I, Scincidae, Gekkonidae and Pygopodidae (Reptilia). Acta Zool, 77: 85–100
Liu Y. Z., Zhang Y. P. 2004. Ultrastructure of spermatozoa of Eumeces elegans. Zool Res, 25(5): 429–435 (In Chinese)
Oliver S. C., Jamieson B. G. M., Scheltinga D. M. 1996. The ultrastructure of spermatozoa of Squamata, II, Agamidae, Varanidae, Colubridae, Elapidae, and Boidae (Reptilia). Herpetologica, 52: 216–241
Phillips D. M., Asa C. S. 1993. Strategies for formation of the midpiece. In Baccetti B. (Ed.), Comparative Spemiauilogy 20 Years After Serono Symp. New York: Raven Press, 75
Rheubert J. L., Siegel D. S., Venable K. J., Sever D. M., Gribbins K. M. 2011. Ultrastructural description of spermiogenesis within the Mediterranean Gecko, Hemidactylus turcicus (Squamata: Gekkonidae). Micron, 42: 680–690
R?ll B., von Düring M. U. G. 2008. Sexual characteristics and spermatogenesis in males of the parthenogenetic gecko Lepidoctylus lugubris (Reptilia, Gekkonidae). Zoology, 5: 385–400
Scheltinga D. M., Jamieson B. G. M., Espinoza R. E., Orrell K. S. 2001. Descriptions of the mature spermatozoa of the lizards Crotaphytus bicinctores, Gambelia wislizenii (Crotaphytidae) and Anolis carolinensis (Polychrotidae) (Reptilia, Squamata, Iguania). J Morph, 247: 160–171
Teixeira R. D., Colli G. R., Báo S. N. 1999a. The ultrastructure of spermatozoa of the lizard Polycbrus acutirostris (Squamata, Polychrotidae). J Submicrosc Cytol Pathol, 31 : 387–395
Teixeira R. D., Colli G. R., Báo S. N. 1999b. The ultrastructure of the spermatozoa of the lizard Micrablepharus maximiliani (Squamata, Gymnophthalmidae), with considerations on the use of sperm ultrastructure characters in phylogenetic reconstruction. Acta Zool, 80: 47–59
Teixeira R. D., Colli G. R., Báo S. N. 1999c. The ultrastructure of the spermatozoa of the worm lizard Amphisbaena alba (Squamata, Gymnophthalmidae), and the phylogenetic relationships of amphisbaenians. Can J Zool, 77: 1254–1264
Tavares-Bastos L., Colli G. R., Báo S. N. 2008. The evolution of sperm ultrastructure among Boidae (Serpentes). Zoomorphology, 127: 189–202
Teixeira R. D., Vieira G. H. C., Colli G. R., Báo S. N. 1999d. Ultrastructural study of spermatozoa of the neotropical lizards , Tropidurus semitaeniatus and Tropidurus torquatus (Squamata, Tropiduridae). Tissue Cell, 31: 308–317
Tourmente M., Cardozo G., Bertona M., Guidobaldi A., Giojalas L., Chiaraviglio M. 2006. The ultrastructure of the spermatozoa of Boa constrictor occidentalis, with considerations on its mating system and sperm competition theories. Acta Zool, 87: 25–32
Tourmente M., Giojalas L., Chiaraviglio M. 2008. Sperm ultrastructure of Bothrops alternatus and Bothrops diporus (Viperidae, Serpentes), and its possible relation to the reproductive features of the species. Zoomorphology, 127: 241–248
Teixeira R. D., Scheltinga D. M., Trauth S. E., Colli G. R., Báo S. N. 2002. A comparative ultrastructural study of spermatozoa of the teiid lizards Cnemidophorus gularis gularis, Cnemidophorus ocellifer, and Kentropyx altamazonica (Reptilia, Squamata, Teiidae). Tissue Cell, 34 (3): 135–142
Tavares-Bastos L., Teixeira R. D., Colli G. R., Báo S. N. 2002. Polymorphism in the sperm ultrastructure among four species of lizards in the genus Tupinambis (Squamata: Teiidae). Acta Zool, 83: 297–307
Vieira G. H. C., Colli G. R., Báo S. N. 2004. The ultrastructure of the spermatozoon of the lizard Iguana iguana (Reptilia, Squamata, Iguanidae) and the variability of sperm morphology among iguanian lizards. J Anat, 204 (6): 451–464
Vieira G. H. C., Colli G. R., Báo S. N. 2005. Phylogenetic relationships of corytophanid lizards (Iguania, Squamata, Reptilia) based on partitioned and total evidence analyses of sperm morphology, gross morphology, and DNA data. Zool Scr, 34: 605–625
Vieira G. H. C., Cunha L. D., Scheltinga D. M., Glaw F., Colli G. R., Báo S. N. 2007. Sperm ultrastructure of hoplocercid and oplurid lizards (Sauropsida, Squamata, Iguania) and the phylogeny of Iguania. J Zool Syst Evol Res, 45(3): 230–241
Zhang Y. P., Fang Z. X., Ji X. 2006. A comparison of the ultrastructure of spermatozoa of two species of skinks Mabuya multifasciata and Sphenomorphus indicus. Acta Zool Sin, 52(3): 591–602 (In Chinese)
Zhang Y. P., Hu J. R., Ji X. 2004. Ultrastructure of spermatozoa of the Chinese skink Eumeces chinensis. Acta Zool Sin, 50 (3): 431–441 (In Chinese)
Zhang Y. P., Ying X. P., Ji X. 2005. Ultrastructure of spermatozoon of the northern grass lizard (Takydromus septentrionalis) with comments on the variability of sperm morphology among lizard taxa. Zool Res, 26(5): 518–526 (In Chinese)
Zhao E. M., Zhao K. T., Zhou K. Y. 1999. Fauna Sinica: Reptilia, Vol. 2, Squamata: Lacertilia. Beijing: Science Press (In Chinese)

更新日期/Last Update: 2016-01-25