[1].[J].Asian Herpetological Research,2014,5(3):150-160.[doi:10.3724/SP.J.1245.2014.00150]
 Jian ZHAO,Jianhuan YANG,Guoling CHEN,et al.Description of a New Species of the Genus Brachytarsophrys Tian and Hu, 1983 (Amphibia: Anura: Megophryidae) from Southern China Based on Molecular and Morphological Data[J].Asian Herpetological Research(AHR),2014,5(3):150-160.[doi:10.3724/SP.J.1245.2014.00150]


Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Original Article


Description of a New Species of the Genus Brachytarsophrys Tian and Hu, 1983 (Amphibia: Anura: Megophryidae) from Southern China Based on Molecular and Morphological Data
Jian ZHAO1 Jianhuan YANG2 Guoling CHEN1 Chunquan CHEN3 and Yingyong WANG1*
1 State Key Laboratory of Biocontrol / The Museum of Biology, School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, Guangdong, China
2 Kadoorie Conservation China, Kadoorie Farm and Botanic Garden, Lam Kam Road, Tai Po, Hong Kong SAR, China
3 Administrative Bureau of Jinggangshan, Jinggangshan 343600, Jiangxi, China
Megophryidae Brachytarsophrys popei sp. nov. morphology mitochondrial DNA taxonomy
A new species, Brachytarsophrys popei sp. nov., is described based on a series of specimens collected from Mount Jinggang, Jiangxi Province, Taoyuandong Nature Reserve, Hunan Province and Nanling Nature Reserve, Guangdong Province, China. The new species can be easily distinguished from other known congeners by morphology, morphometrics and molecular data of the mitochondrial 16S rRNA gene. It is characterized by its relatively small size with 86.2 mm in snout-vent length in adult female and 70.7 mm–83.5 mm in males; vomerine teeth bearing on two markedly elevated ridges, which projecting behind far beyond the posterior level of the choanae, widely separated by a distance nearly 1.5 times length of one; margin of tongue deeply notched behind; toes about one-third to two-thirds webbed in males, at most one-third webbed in female; the webs extending as a wide fringes along either side of toes; upper eyelid with tubercles, one of which is enlarged and becoming a remarkably prominent, bluntly conical light-yellow horn; black tiny nuptial spines on the dorsal surface of the first finger and second finger base, single vocal sac in males; gravid females bear pure yellowish oocytes; tadpoles with a transverse white stripe on ventral surface and two longitudinal white stripes along the sides of body. The new species represents the fifth known Brachytarsophrys species.


Boulenger G. A. 1889. Description of a new batrachian of the genus Leptobrachium, obtained by M. L. Fea in the Karens Mountains, Burma. Annali del Museo Civico de Storia Naturale di Genova, Ser. 2(7): 748–750
Boulenger G. A. 1890. The Fauna of British India, including Ceylon and Burma, Reptilia and Batrachia. London: Taylor and Francis, 541 pp
Boulenger G. A. 1908. A revision of the Oriental pelobatid batrachians (genus Megalophrys). Proc Zool Soc London, 1908: 439–440
Fei L., Hu S. Q., Ye C. Y., Huang Y. Z. 2009. Fauna Sinica. Amphibia, Vol. 2, Anura Ranidae. Beijing, China: Science Press (In Chinese)
Fei L., Ye C. Y. 2001. The Colour Handbook of the Amphibians of Sichuan. Beijing, China: Chinese Forestry Press (In Chinese)
Felsenstein J. 2004. Inferring phylogenies. Sunderland, MA: Sinauer Associates
Frost D. R., Grant T., Faivovich J., Bain R. H., Haas A., Haddad C. F., Wheeler W. C. 2006. The amphibian tree of life.?B Am Mus Nat Hist, 297: 1–291
Frost D. R. 2014. Amphibian Species of the World: an Online Reference. Version 5.5 (3 January 2013). Electronic Database accessible at http://research.amnh.org/vz/herpetology/amphibia/index.html. New York: American Museum of Natural History
Gosner K. L. 1960. A simplified table for staging Anuran embryos and larvae with notes on identification. Herpetologica, 16: 183–190
Posada D., Buckley T. R. 2004. Model selection and model averaging in phylogenetics: Advantages of Akaike information criterion and bayesian approaches over likelihood ratio tests. Syst Biol, 53: 793–808
Posada D., Crandall K. A. 2001. Selecting models of nucleotide substitution: an application to human immunodeficiency virus 1 (HIV-1). Mol Biol Evol, 18: 897–906
Pyron R. A., Wiens J. J. 2011. A large-scale phylogeny of Amphibia including over 2,800 species, and a revised classification of extant frogs, salamanders, and caecilians. Mol Phyl Evol, 61: 543–583
Rao D. Q., Yang D. T. 1997. The variation in karyotypes of Brachytarsophrys with a discussion of the classification of the Genus. Asiatic Herpetol Res, 7: 103–107
Ronquist F., Huelsenbeck J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformat, 19: 1572–1574
Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual. New York: Cold Spring Harbor Laboratory Press, 125pp
Smith M. A. 1921. New or little-known reptiles and batrachians from southern Annam (Indo-China). Proc Zool Soc London, 1921: 423–440
Swofford D. L. 2003. PAUP*: Phylogenetic analysis using parsimony (and Other Methods), Version 4.0b10. Sunderland, MA: Sinauer Associates
Taylor E. H. 1962. The amphibian Fauna of Thailand. Univ Kansas Sci Bull , 43: 284–302
Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. Mol Biol Evol, 28: 2731–2739
Tian Y. Z., Gu X. M., Sun A. Q. 2000. A new species of Megophrys in China ( Amphibia: Pelobatidae). Acta Zoota Sin, 25: 462–466
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acid Res, 25: 4876–4882
Xie F., Wang Z. W. 2000. Review of the systematics of pelobatids. Cultum Herpetol Sin, 8: 356–370

更新日期/Last Update: 2016-01-25