[1].Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China[J].Asian Herpetological Research,2011,2(2):61-71.[doi:10.3724/SP.J.1245.2011.00061]
 Fang YAN,Ke JIANG,Hongman CHEN,et al.Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China[J].Asian Herpetological Research(AHR),2011,2(2):61-71.[doi:10.3724/SP.J.1245.2011.00061]

Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China()

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Original Article


Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China
Fang YAN1 Ke JIANG1 Hongman CHEN21 Ping FANG21 Jieqiong JIN1 Yi LI3 Shaoneng WANG4 Robert W. MURPHY1
1 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
2 Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, Yunnan, China
3 Jiemuxi National Nature Reserve, Yuanling 419600, Hunan, China
4 Maoershan National Nature Reserve, Guilin 541316, Guangxi, China
5 Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada
Rana jiemuxiensis Rana japonica group phylogeny genealogy DNA barcode cryptic species
Fieldwork in Jiemuxi National Nature Reserve, Hunan, China, discovered morphologically indistinguishable groups of brown frogs that bred at different, exclusive times of the year. A principal components analysis of morphometric data, molecular analyses, and the exclusive breeding season suggested the occurrence of two species. The population that breeds during the winter was found to be an undescribed species and was subsequently described as Rana jiemuxiensis sp. nov. The new cryptic species can be identified from its congeners at the same locality by having a different breeding season and a divergent DNA barcode. Six major lineages of brown frogs were resolved with high nodal support. Rana japonica, R. chaochiaoensis, R. omeimontis, and R. jiemuxiensis have independent maternal lineages. Rana hanlunica and R. maoershanensis have essentially identical maternal lineages and they appear to represent the same species. The validity of the species R. longicrus, R. zhenhaiensis, and R. culaiensis and their genealogical relationships are not resolved and deserve further study. The genealogy indicates that sympatric cryptic lineages probably widely exist in the R. longicrus group. This highlights the importance of future more fine-scaled samplings and the inclusion of nuclear genes.


Bossuyt F., Milinkovitch M. C. 2000. Convergent adaptive radiations in Madagascan and Asian ranid frogs reveal covariation between larval and adult traits. PNAS, 97: 6585–6590
Che J., Pang J. F., Zhao E. M., Matsui M., Zhang Y. P. 2007. Phylogenetic relationships of the Chinese brown frogs (Genus Rana) inferred from partial mitochondrial 12S and 16S rRNA gene sequences. Zool Sci, 24: 71–80
Fei L., Hu S. Q., Ye C. Y., Huang Y. Z. 2009. Fauna Sinica. Amphibia. Volume 2. Anura. Beijing, China: Science Press, 957 pp
Frost D. R. 2010. Amphibian Species of the World: An Online Reference. Version 5.4, American Museum of Natural History, NY. Available from: http://research.amnh.org/vz/herpetology/amphibia/ (accessed 8 April, 2010).
Hebert P. D. N., Ratnasingham D., Ward J. R. 2003. Barcoding animal life: Cytochrome c oxidase subunit I divergences among closely related species. Proc Roy Soc London, Series B (Suppl.)
Jiang J. P., Zhou K. Y. 2001. Phylogenetic relationships of Chinese brown frogs inferred from mitochondrial DNA sequence of 12S ribosome RNA gene. Zool Res, 22: 27–32
Li P. P., Lu Y. Y., Li A. 2008. A new species of brown frog from Bohai, China. Asiatic Herpetol Res, 11: 62–70
Linnaeus C. 1758. Systema naturae per regna tria naturae, secundum classes, ordines, genera, species, cum characteribus, differentiis, synonymis, locis. Tomus I. Editio decimal, reformata. Holmiae: Laurentii Salvii, 824 pp
Liu C. C. 1946. A new wood frog from West China, Rana chaochiaoensis, with a discussion of its allied species. J West China Border Res Soc, 16(B): 7–14
Liu C. C., Hu S. Q. 1961. Chinese Tailless Amphibians. Peking (Beijing): Science Press
Lu Y. Y., Li P. P. 2002. A new wood-frog of the genus Rana in Mt. Kunyu, Shandong Province, China (Amphibian: Anura: Ranidae). Acta Zoo Sin, 27: 162–166
Lu Y. Y., Li P. P., Jiang D. B. 2007. A new species of Rana (Anura, Ranidae) from China. Acta Zoo Sin, 32: 792–801
Lu Y. Y., Li P. P., Jiang D. B., Zhang J. H., Luo Y. Z., Wang S. N. 2006. A new record of amphibian, Rana chaochiaoensis, in Guangxi Autonomous Region. Sichuan J Zool, 25: 267
Nylander J. A. 2004. MrModeltest v2. (program distributed by the author). Evolutionary Biology Centre, Uppsala: Uppsala University
Pope C. H., Boring A. M. 1940. A survey of Chinese amphibia. Peking Nat Hist Bull, 15: 13–86
Ronquist F., Huelsenbeck J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 1: 1572–1574
Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular Cloning: A Laboratory Manual, Sec Ed. Cold Springs Harbor, NY: Cold Spring Harbor Laboratory Press
Shen Y. H., Jiang J. P., Yang D. D. 2007. A new species of the genus Rana—Rana hanluica sp. nov. from Hunan Province, China (Anura: Ranidae). Acta Zool Sin, 53: 481–488
Smith M. A., Poyarkov N. A., Hebert P. D. N. 2008. COI DNA barcoding amphibians: Take the chance, meet the challenge. Mol Ecol, 8: 235–246
Swofford D. L. 2003. PAUP*: Phylogenetic Analysis Using Parsimony (and Other Methods) version 4b10. Sunderland, MA: Sinauer Associates
Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Bio Evol, 24: 1596–1599
Tanaka-Ueno T., Matsui M., Sato T., Takenaka S., Takenaka O. 1998. Phylogenetic relationships of brown frogs with 24 chromosomes from Far East Russia and Hokkaido assessed by mitochondrial cytochrome b gene sequences (Rana: Ranidae). Zool Sci, 15: 289–294
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 25: 4876–4882
Yang B, Lu Y, Li P. 2011. Discussion on validity of Rana maoershanensis based on partial sequence of 16S rRNA gene. Asian Herpetol Res, 1: 97–102
Yang X. G., Wang Y. Q., Zhou K. Y., Liu Z. Q. 2001. Phylogenetic relationships of Chinese brown frogs (Rana) based on sequence of mitochondrial cytochrome b gene. Zool Res, 22: 345–350
Yang J. X., Zhou W. W., Rao D. Q., Poyarkov A. N., Kuzmin S. L., Che J. 2010. Validity and systematic position of Rana altaica (Rana: Ranidae): Results of a phylogenetic analysis. Zool Res, 31: 353–360

更新日期/Last Update: 2016-03-15