[1].Phylogenetic Analyses Reveal a Unique Species of Elaphe (Serpentes, Colubridae) New to Science[J].Asian Herpetological Research,2010,1(2):90-96.[doi:10.3724/SP.J.1245.2010.00090]
 LING Chen,*,LIU Shaoying,et al.Phylogenetic Analyses Reveal a Unique Species of Elaphe (Serpentes, Colubridae) New to Science[J].Asian Herpetological Research(AHR),2010,1(2):90-96.[doi:10.3724/SP.J.1245.2010.00090]

Phylogenetic Analyses Reveal a Unique Species of Elaphe (Serpentes, Colubridae) New to Science()

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Original Article


Phylogenetic Analyses Reveal a Unique Species of Elaphe (Serpentes, Colubridae) New to Science
LING Chen 1* LIU Shaoying 2* HUANG Song 1** Frank T. Burbrink3 GUO Peng4 SUN Zhiyu2 and ZHAO Jie2
1 College of Life and Environment Sciences, Huangshan University, Huangshan 245021, Anhui, China
2 Sichuan Academy of Forestry, Chengdu 610066, Sichuan, China
3 Department of Biology, College of Staten Island, The City University of New York, 2800 Victory Blvd, Staten Island, NY 10314, USA
4 Department of Life Sciences and Food Engineering, Yibin University, Yibin 644000, Sichuan, China
Elaphe ratsnake phylogenetic analysis mitochondrial gene genetic distance
The snakes comprising the monophyletic group referred to as ratsnakes are found throughout Asia, Europe and the New World. Recently, three snake samples likely belonging to the ratsnakes were collected in Zoige County, Sichuan Province, China. Species identity was difficult to delimit morphologically because the specimens were juveniles and partially damaged. Subsequently, a molecular phylogenetic approach was used. Portions of three mitochondrial genes (cyt b, ND4 and 12S rRNA) were sequenced and analyzed. The results showed that they were sister to the genus Elaphe. Very little genetic variation was found among the three samples. The minimum genetic distances between these samples and those within Elaphe were greater than any currently recognized species within the genus. We conclude that this likely represents a new species within the genus Elaphe. Adult specimens and a morphologic description are needed for further study.


Arévalo E., Davis S. K., Sites J. W., Jr. 1994. Mitochondrial DNA sequence divergence and phylogenetic relationships among eight chromosome races of the Sceloporus grammicus complex (Phrynosomatidae) in Central Mexico. Syst Biol, 43: 387?418
Burbrink F. T., Lawson R., Slowinski J. B. 2000. Mitochondrial DNA phylogeography of the polytypic North American rat snake (Elaphe obsoleta): A critique of the subspecies concept. Evolution, 54: 2107?2118
Burbrink F. T., Lawson R. 2007. How and when did Old World ratsnakes disperse into the New World? Mol Phyl Evol, 43: 173?189
Burbrink F. T., Crother B. I. 2010. Evolution and taxonomy of snakes. In Reproductive Biology and Phylogeny of Snakes (Aldridge and Sever, eds). New Hampshire: Science Publishers, Inc (In review/press)
Burbrink F. T., Pyron R. A. 2010. How does ecological opportunity influence rates of speciation, extinction, and morphological diversification in New World ratsnakes (Tribe Lampropeltini)? Evolution, 64: 934?943
de Queiroz A., Lawson R., Lemos-Espinal J. A. 2002. Phylogenetic relationships of North American garter snakes (Thamnophis) based on four mitochondrial genes: How much DNA sequence is enough? Mol Phyl Evol, 22: 315?329
Huang S., Liu S. Y., Guo P., Zhang Y. P., Zhao E. M. 2009. What are the closest relatives of the hot-spring snakes (Colubridae, Thermophis), the relict species endemic to the Tibetan Plateau? Mol Phyl Evol, 51: 438?446
Huelsenbeck J. P., Ronquist F. 2001. MRBAYES: Bayesian inference of phylogenetic trees. Bioinformatics, 17: 754?755
Kelly C. M. R., Barker N. P., Villet M. H. 2003. Phylogenetics of advanced snakes (Caenophidia) based on four mitochondrial genes. Syst Biol, 52: 439?459
Lawson R., Slowinski J. B. Crother B. I., Burbrink F. T. 2005. Phylogeny of Colubroidea (Serpentes): New evidence from mitochondrial and nuclear genes. Mol Phyl Evol, 37: 581?601
Malhotra A., Thorpe R. S. 2004. A phylogeny of four mitochondrial gene regions suggests a revised taxonomy for Asian pitvipers (Trimeresurus and Ovophis). Mol Phyl Evol, 32: 83?100
Nagy Z. T., Lawson R., Joger U., Wink M. 2004. Molecular systematics of racers, whipsnakes and relatives (Reptilia: Colubridae) using mitochondrial and nuclear markers. J Zool Syst Evol Res, 42: 223?233
Pope C. H. 1935. The Reptiles of China. Turtles, Crocodilians, Snakes, Lizards. New York: The American Museum of Natural History, Natural History of Central Asia, V: 266?271
Posada D., Crandall K. A. 1998. Modeltest: Testing the model of DNA substitution. Bioinformatics, 14: 817?818
Pyron R. A., Burbrink F. T. 2009. Lineage diversification in a widespread species: Roles for niche divergence and conservatism in the common kingsnake, Lampropeltis getula. Mol Ecol, 18: 3443?3457
Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular cloning: A laboratory manual, second ed., New York: Cold Spring Harbor Laboratory Press
Schulz K. D. 1996. A monograph of the colubrid snakes of the genus Elaphe Fitzinger. Havlickuv Brod, Czech Republic: Koeltz Scientific Books
Stamatakis A., Hoover P., Rougemont J. 2008. A rapid bootstrap algorithm for the RAxML Web-Servers. Syst Biol, 75: 758?771
Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular Evolutionary Genetics Analysis (MEGA) software version 4.0. Mol Biol Evol, 24: 1596?1599
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL_X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 25: 4876?4882
Ursenbacher S., Conelli A., Golay P., Monney J. C., Zuffi M. A., Thiery G., Durand T., Fumagalli L. 2006. Phylogeography of the asp viper (Vipera aspis) inferred from mitochondrial DNA sequence data: Evidence for multiple Mediterranean refugial areas. Mol Phyl Evol, 38: 546?552
Utiger U., Helfenberger N., Schatti B., Sch?tti C., Ruf M., Ziswiler V. 2002. Molecular systematics and phylogeny of Old World and New World ratsnakes, Elaphe Auct., and related genera (Reptilia, Squamata, Colubridae). Russ J Herpetol, 9: 105?124
Zaher H., Grazziotin F. G., Cadle J. E., Murphy R. W., de Moura-Leite J. C. D., Bonatto S. L. 2009. Molecular phylogeny of advanced snakes (Serpentes, Caenophidia) with an emphasis on South American Xenodontines: A revised classification and descriptions of new taxa. Pap Avulsos Zool, 49: 115?153
Zhao E. M. 2003. Coloured Atlas of Sichuan Reptiles. Beijing: Forestry Publishing House, 292 pp (In Chinese)
Zhao E. M. 2006. Snakes in China, Vol. 1, Hefei: Anhui Science and Technology Publishing House (In Chinese)

更新日期/Last Update: 2016-03-15