Chairat TANTRAWATPAN,Weera THONGNETR,Warayutt PILAP,et al.Genetic Diversity and Population Structure of the Oriental Garden Lizard, Calotes versicolor Daudin, 1802 (Squamata: Agamidae) along the Mekong River in Thailand and Lao PDR[J].Asian Herpetological Research(AHR),2021,12(1):49-57.[doi:10.16373/j.cnki.ahr.200046]
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Genetic Diversity and Population Structure of the Oriental Garden Lizard, Calotes versicolor Daudin, 1802 (Squamata: Agamidae) along the Mekong River in Thailand and Lao PDR
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2021 VoI.12 No.01
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Genetic Diversity and Population Structure of the Oriental Garden Lizard, Calotes versicolor Daudin, 1802 (Squamata: Agamidae) along the Mekong River in Thailand and Lao PDR
Chairat TANTRAWATPAN1 Weera THONGNETR2 Warayutt PILAP2 Warong SUKSAVATE3 Takeshi AGATSUMA4 Wittaya TAWONG5 Trevor N. PETNEY6 Weerachai SAIJUNTHA2*
1Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine and Center of Excellence in Stem Cell Research, Thammasat University, Pathumthani 12120, Thailand
2 Walai Rukhavej Botanical Research Institute, Biodiversity and Conservation Research Unit, Mahasarakham University, Maha Sarakham 44150, Thailand
3 Department of Forest Biology, Faculty of Forestry, Kasetsart University, Bangkok 10900, Thailand
4 Division of Environmental Health Sciences, Kochi Medical School, Kochi University, Oko, Nankoku 783-8505, Japan
5 Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
6 Departments of Zoology and Paleontology and Evolution, State Museum of Natural History Karlsruhe, Karlsruhe 76133, Germany
Agamid CO1 haplotype lizard phylogeny reptile
Calotes versicolor Daudin, 1802, is geographically widespread along the Mekong River basin. The Mekong River is play important role as a significant natural barrier to several terrestrial animals living on different sides. This study aims to analyze the genetic diversity and population structure of C. versicolor populations collected from different sides of Mekong River using mitochondrial cytochrome c oxidase subunit 1 (CO1) sequences. We obtained sequences of 200 individuals from 18 sampling localities from left and right sides of the Mekong River in Lao PDR and Thailand respectively. Overall, 91 haplotypes were detected, which reflect high levels of genetic diversity in this species at the study areas. Haplotype network and phylogenetic analyses revealed that there were six major lineages (lineage C – lineage H) of C. versicolor populations within the Mekong River, whereas lineages A and B have previously been found from China and Vietnam. The genetic distance among C. versicolor was significantly related to spatial distance, however, the Mekong River had no significant effect on genetic distance. Our findings, together with previous studies, suggests that C. versicolor in Asia is a species complex with other cryptic lineages being likely but there is a need for further exploration. Thus, comprehensive genetic, biological and ecological studies of C. versicolor should be conducted throughout its entire distribution range.


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Last Update: 2021-03-25