Janak Raj KHATIWADA,Bin WANG,Tian ZHAO,et al.An Integrative Taxonomy of Amphibians of Nepal: An Updated Status and Distribution[J].Asian Herpetological Research(AHR),2021,12(1):1-35.[doi:10.16373/j.cnki.ahr.200050]
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An Integrative Taxonomy of Amphibians of Nepal: An Updated Status and Distribution
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2021 VoI.12 No.01
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An Integrative Taxonomy of Amphibians of Nepal: An Updated Status and Distribution
Janak Raj KHATIWADA Bin WANG Tian ZHAO Feng XIE Jianping JIANG*
CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China
amphibian distribution molecular phylogenetics Nepal species diversity taxonomy
Amphibians are declining worldwide. At the same time, each year many species of amphibians are described, indicating that biological diversity on Earth remains poorly understood. This is especially true for mountainous regions, such as the Himalayas, which are also regarded as a biodiversity hotspot. However, it is most likely that the current diversity of amphibians in Nepal is highly underestimated. Amphibian inventories by the past researchers primarily used phenotypic characters as the basis for species identification. However, the application of molecular systematics methods to taxonomic studies has successfully uncovered the taxonomic status of several cryptic taxa. This study used both molecular (16S rDNA sequences) and morphological comparisons and estimated the diversity of amphibians from Nepal. This study reports the occurrence of 38 species of amphibians based on both molecular and morphological comparisons. Among them, three species were recorded for the first time in Nepal. This study also invalids the taxonomic status of Sphaerotheca paschima. This study highlights the importance of integrative taxonomic approaches that help to resolve the current taxonomic ambiguities of Nepalese amphibians.


Alam M. S., Igawa T., Khan M. M. R., Islam M. M., Kuramoto M., Matsui M., Kurabayashi A., Sumida M. 2008. Genetic divergence and evolutionary relationships in six species of genera Hoplobatrachus and Euphlyctis (Amphibia: Anura) from Bangladesh and other Asian countries revealed by mitochondrial gene sequences. Mol Phylogenet Evol, 48(2): 515?527
Amphibiaweb. 2020. Information on amphibian biology and conservation. AmphibiaWeb. AmphibiaWeb, Berkeley, California
Araújo M. B., Thuiller W., Pearson R. G. 2006. Climate warming and the decline of amphibians and reptiles in Europe. J Biogeogr, 33(10): 1712?1728
Biju S., Garg S., Mahony S., Wijayathilaka N., Senevirathne G., Meegaskumbura M. 2014. DNA barcoding, phylogeny and systematics of Golden-backed frogs (Hylarana, Ranidae) of the Western Ghats-Sri Lanka biodiversity hotspot, with the description of seven new species. Contrib Zool, 83(4): 269?335
Blyth E. 1852. Report of curator. J Asiatic Soc Ben, 21: 341–358
Boulenger G. A., Annadale N., Wall F., Regan C. T. 1907. Reports on a Collection of Batrachia, Reptiles, and Fish from Nepal and the Western Himalayas. Rec Ind Mus, 1: 151?155
Camilo M., Tittensor D. P., Adl S., Simpson A. G. B., Worm B. 2011. How Many Species Are There on Earth and in the Ocean? PloS Biol, 9: e1001127
Ceriaco L. M., Gutierrez E. E., Dubios A. 2016. Photography-based taxonomy is inadequate, unnecessary, and potentially harmful for biological sciences. Zootaxa, 4196(3): 435?445
Chen J.M., Zhou W.W., Poyarkov Jr N. A., Stuart B. L., Brown R. M., Lathrop A., Wang Y.Y., Yuan Z.Y., Jiang K., Hou M. 2017. A novel multilocus phylogenetic estimation reveals unrecognized diversity in Asian horned toads, genus Megophrys sensu lato (Anura: Megophryidae). Mol Phylogenet Evol, 106(2017): 28?43
Costello M. J., May R. M., Stork N. E. 2013. Can we name Earth’s species before they go extinct? Science, 339(6118): 413?416
Das I. 1998. A new species of Rana from the Terai of Nepal. J Herpetol, 32(2): 223?229
Davidson C. 2004. Declining downwind: amphibian population declines in California and historical pesticide use. Ecol Appl, 14(6): 1892?1902
Deuti K., Grosjean S., Nicolas V., Vasudevan K., Ohler A. 2017. Nomenclatural puzzle in early Xenophrys nomina (Megophryidae, Amphibia) solved with description of two new species from India (Darjeeling hills and Sikkim). Alytes, 34(1?4): 20?48
Dinesh K., Radhakrishnan C., Gururaja K., Bhatta G. 2009. An annotated checklist of amphibian of India with some insights into the patterns of species discoveries, distribution and endemism. Zoological Survey of India, Dehra Dun, India
Dubois A. 1973. Diagnoses of 3 new species of amphibia from Nepal. Bullet Societ Zoolog France Evol Zool, 98: 495?497
Dubois A. 1974a. Diagnoses de trois espèces nouvelles d’Amphibiens du Nepal. Bullet Societ Zoolog France Evol Zool, 98: 495?497
Dubois A. 1974b. Research on Zoogeography of Nepalese Amphibians. Pages 788?791 in Bulletin De La Societe Zoologique De France?Evolution Et Zoologie. Soc Zoologique France Inst Oceanographique 195 Rue Saint?Jacques, 75005 Paris, France, Paris, France
Dubois A. 1974 Diagnoses de trois espèces nouvelles d’amphibiens du Népal. Bullet Societ Zoolog France Evol Zool, 98: 495?497
Dubois A. 1975a. Un nouveau sous-genre (Paa) et trois nouvelles espèces du genre Rana. Remarques sur la phylogénies des Ranidés (Amphibiens, Anoures). Comptes Rendus Hebdomadaires des Séances de l’Académie des Sciences. Paris Serie 3, Zoologie 324: 1093–1115
Dubois A. 1975b. Une nouveau complexe d’espèces jumelles distinguées par le chant: les grenouilles de Népal voisines de Rana limnocharis Boie (Amphibienes, Anoures). Comptes Rendus Hebdomadaires des Séances de l’Académie des Sciences Paris 281: 1717?1720
Dubois A. 1977. Chants et écologie chez les Amphibiens du Népal. Pages 109?118 in Himalaya Ecolethnologie Colloq Int CNRS
Dubois A. 1984. Note preliminaire sur le groupe de Rana limnocharis Gravenhorst, 1829 (Amphibiens, Anoures). Alytes Paris, 3: 143?159
Dubois A. 1987. Miscellanea taxinomica batrachologica. Alytes Paris, 5: 7?95
Dubois A. 1999. South Asian Amphibia: a new frontier for taxonomists. J South Asian Nat His, 4:1?11
Dubois A., Matsui M. 1983. A new species of frog (genus Rana, subgenus Paa) from western Nepal (Amphibia: Anura). Copeia, 1983(4): 895?901
Dutta D., Das S. K. 2013. Phylogenetic relationship of Duttaphrynus melanostictus from India and China as revealed from the study of 12S and 16S mtDNA genes. Int J Zool Res, 9(2): 49?57
Gallant A. L., Klaver R. W., Casper G. S., Lannoo M. J. 2007. Global rates of habitat loss and implications for amphibian conservation. Copeia, 2007(4): 967?979
Garg S., Senevirathne G., Wijayathilaka N., Phuge S., Deuti K., Manamendra-Arachchi K., Meegaskumbura M., Biju S.D. 2018. An integrative taxonomic review of the South Asian microhylid genus Uperodon. Zootaxa, 4384(1): 1?88
Günther A. 1858. Catalogue of colubrine snakes in the collection of the British Museum. British Museum, London
Günther A. 1860. Contribution to a knowledge of the reptiles of the Himalaya Mountains. Pro Zool Soc Lon, 1860: 148?175
Günther, A. 1861. List of the cold-blooded vertebrata collected by BH Hodgson in Nepal. Pro Zool Soc Lon, 43(1): 224–234
Günther A. 1875. Second report on collections of Indian reptiles obtained by the British Museum. Pro Zool Soc Lon, 43(1): 224–234
Hillers A., Veith M., R?del M. O. 2008. Effects of forest fragmentation and habitat degradation on West African leaf‐litter Frogs. Conserv Biol, 22(3): 762?772
Hoffmann M., Hilton-Taylor C., Angulo A., B?hm M., Brooks T. M., Butchart S. H., Carpenter K. E., Chanson J., Collen B., Cox N. A. 2010. The impact of conservation on the status of the world’s vertebrates. Science, 330(6010): 1503?1509
Hofmann S., St?ck M., Zheng Y., Ficetola F. G., Li J.T., Scheidt U., Schmidt J. 2017. Molecular phylogenies indicate a paleo-tibetan origin of Himalayan lazy toads (Scutiger). Sci Rep, 7(1): 3308
Houlahan J. E., Findlay C. S. 2003. The effects of adjacent land use on wetland amphibian species richness and community composition. Can J Fish Aquat Sci, 60(9): 1078?1094
Howlader M. S. A., Nair A., Gopalan S. V., Meril? J. 2015. A New Species of Euphlyctis (Anura: Dicroglossidae) from Barisal, Bangladesh. PloS One 10(2): e0116666
IUCN. 2001. IUCN Red List categories and criteria: Version 3.1. International Union for Conservation of Nature Natural Resources, IUCN Species Survival Commission, Gland, Switzerland and Cambridge, UK
Khatiwada J. R., Wang B., Ghimire S., Vasudevan K., Paudel S., Jiang J.P. 2015. A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from Eastern Himalaya. Asian Herpetol Res, 6(4): 245–256
Khatiwada J. R., Ghimire S., Paudel Khatiwada S., Paudel B., Bischof R., Jiang J. P., Haugaasen T. 2016. Frogs as potential biological control agents in the rice fields of Chitwan, Nepal. Agri Ecosys Environ, 230(2016): 307–31
Khatiwada J. R., Shu G. C., Wang S. H., Thapa A., Wang B., Jiang J. P. 2017. A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal. Zootaxa, 4254(2): 221–239
Khatiwada J. R., Shu G., Subedi T. R., Wang B., Ohler A., Canatella D. C., Xie F., Jiang J. P. 2019a. A New Species of Megophryid Frog of the Genus Scutiger from Kangchenjunga Conservation Area, Eastern Nepal. Asian Herpetol Res, 10(3): 139–157
Khatiwada J. R., Zhao T., Chen Y., Wang B., Xie F., Cannatella D. C., Jiang J.P. 2019b. Amphibian community structure along elevation gradients in eastern Nepal Himalaya. BMC Ecol, 19(2019):19
Khatiwada J. R., Shu G. C., Wang B., Zhao T., Xie F., Jiang J. P. 2020. Description of a new species Amolops Cope, 1865 (Amphibia: Ranidae) from Nepal and nomenclatural validation of Amolops nepalicus Yang, 1991. Asian Herpetol Res, 11(2): 1?40
K?hler J., Vieites D. R., Bonett R. M., García F. H., Glaw F., Steinke D., Vences M. 2005. New amphibians and global conservation: a boost in species discoveries in a highly endangered vertebrate group. BioScience 55(8): 693?696
Kumar S., Stecher G., Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol, 33(7): 1870–1874
Leviton A. E., G. S. Myers, and L. W. Swan. 1956. Zoological Results of the California Himalayan Expedition to Makalu, Eastern Napal: I. Amphibians and Reptiles. Natural History Museum of Stanford University, USA
Li J. T., Li Y., Murphy R. W., Rao D. Q., Zhang Y.P. 2012. Phylogenetic resolution and systematics of the Asian tree frogs, Rhacophorus (Rhacophoridae, Amphibia). Zool Scr, 41(6): 557?570
Mace G. M. 2004. The role of taxonomy in species conservation. Philos Trans R Soc Lond B Biol Sci, 359(1444): 711?719
Mahony S., Kamei R. G., Teeling E. C., Biju S. D. 2018. Cryptic diversity within the Megophrys major species group (Amphibia: Megophryidae) of the Asian Horned Frogs: Phylogenetic perspectives and a taxonomic revision of South Asian taxa, with descriptions of four new species. Zootaxa, 4523(1): 1–96
Matsui M., Ito H., Shimada T., Ota H., Saidapur S. K., Khonsue W., Tanaka-Ueno T., Wu G. F. 2005. Taxonomic relationships within the pan oriental narrow mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae). Zool Sci 22(4): 489?495
Mayr E. 1969. Principles of systematic zoology. McGraw-Hill Book Company, New York
Meegaskumbura M., Meegaskumbura S., Bowatte G., Manamendra-Arachchi K., Pethiyagoda R., Hanken J., Schneider C. 2011. Taruga (Anura: Rhacophoridae), a new genus of foam-nesting tree frogs endemic to Sri Lanka. Ceylon J Sci, 39(2): 75?94
Miller M. A., W. Pfeiffer, and T. Schwartz. 2010. Creating the CIPRES Science Gateway for inference of large phylogenetic trees. Pages 1–8 in 2010 Gateway Computing Environments Workshop (GCE). San Diego Supercomputer Center, New Orleans, LA, USA.
Mitchell J., Zug G. 1995. Keys to the known amphibians and reptiles of the Royal Chitwan National Park, Nepal. Smithsonian Herpetol Info Ser 106: 1?15.
Nanhoe L. M., and P. E. Ouboter. 1987b. The distribution of reptiles and amphibians in the Annapurna-Dhaulagiri region (Nepal). Zool Verh Leiden, 240: 1–100
Ohler A., Deuti K. 2018. Polypedates smaragdinus Blyth, 1852 - a senior subjective synonym of Rhacophorus maximus Günther, 1858. Zootaxa, 4375(2): 273?280
Oliver L. A., Prendini E., Kraus F., Raxworthy C. J. 2015. Systematics and biogeography of the Hylarana frog (Anura: Ranidae) radiation across tropical Australasia, Southeast Asia, and Africa. Mol Phyl Evol, 90(2015): 176?192
Padhye A., Dahanukar N., Sulakhe S., Dandekar N., Limaye S., Jamdade K. 2017. Sphaerotheca pashchima, a new species of burrowing frog (Anura: Dicroglossidae) from western India. J Threat Taxa, 9(6): 10286?10296
Schleich H. H., K?stle W. 2002. Amphibians and reptiles of Nepal: Biology, Systematics, Field Guide. A. R. G. Gantner Verlag K. G, Koenigstein, Germany
Shah K. B., Tiwari S. 2004. Herpetofauna of Nepal: A Conservation Companion. IUCN Nepal, Kathmandu, Nepal
Shrestha T. K. 1989. Ecological aspects of the life?history of the Himalayan newt, Tylototriton verrucosus (Anderson) with reference to conservation and management. J Bombay Nat Hist Soc, 86(3): 333?338
Smith M., Battersby J. 1953. On a collection of amphibians and reptiles from Nepal. J Nat Hist, 12(6): 702?704
Stamatakis A. 2014. RAxML version 8: a tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics, 30(9): 1312–1313
Stuart S. N., Chanson J. S., Cox N. A., Young B. E., Rodrigues A. S., Fischman D. L., Waller R. W. 2004. Status and trends of amphibian declines and extinctions worldwide. Science, 306(5702): 1783?1786
Swan L. W., Leviton A. E. 1962. The herpetology of Nepal: a history, check list, and zoogeographical analysis of the herpetofauna. California Academy of Sciences
Taylor H. R., Harris W. E. 2012. An emergent science on the brink of irrelevance: a review of the past 8?years of DNA barcoding. Mol Ecol Res, 12(3): 377?388
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 25(24): 4876–4882
Van Bocxlaer I., Biju S.D., Loader S. P., Bossuyt F. 2009. Toad radiation reveals into-India dispersal as a source of endemism in the Western Ghats-Sri Lanka biodiversity hotspot. BMC Evol Biol, 9(1):131
Yang Z., Rannala B.. 2012. Molecular phylogenetics: principles and practice. Nat Rev Genet, 13(5): 303?314
Zug G., Mitchell J. 1995. Amphibians and reptiles of the Royal Chitwan National Park, Nepal. Asiatic Herpetol Res, 6: 172?180


Last Update: 2021-03-25