Eunbin KIM,Hacheol SUNG,Donghyun LEE,et al.Nondestructive Skeletal Imaging of Hyla suweonensis Using Micro-Computed Tomography[J].Asian Herpetological Reserch(AHR),2017,8(4):235-243.[doi:10.16373/j.cnki.ahr.170069]
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Nondestructive Skeletal Imaging of Hyla suweonensis Using Micro-Computed Tomography
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2017 VoI.8 No.4
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Nondestructive Skeletal Imaging of Hyla suweonensis Using Micro-Computed Tomography
Eunbin KIM1 Hacheol SUNG2 Donghyun LEE2 Geunjoong KIM2 Dongha NAM2*and Eungsam KIM2*
1 School of Biological Sciences and Biotechnology, Chonnam National University, 77, Yongbong-ro, Bukgu, Gwangju 61186, South Korea
2 Department of Biological Sciences, College of Natural Sciences, Chonnam National University, 77, Yongbong-ro, Bukgu, Gwangju 61186, South Korea
Hyla suweonensis Micro-computed tomography 3D skeletal structure Nondestructive imaging Endangered species Radiation dose.
We successfully obtained 3D skeletal images of Hyla suweonensis, employing a nondestructive method by applying appropriate anesthesia and limiting the radiation dose. H. suweonensis is a tree frog endemic to Korea and is on the list of endangered species. Previous studies have employed caliper-based measurements and two-dimensional (2D) X-ray imaging for anatomical analyses of the skeletal system or bone types of H. suweonensis. In this work we reconstructed three-dimensional (3D) skeletal images of H. suweonensis, utilizing a nondestructive micro-computed tomography (micro-CT) with a short scan and low radiation dose (i.e. 4 min and 0.16 Gy). Importantly, our approach can be applied to the imaging of 3D skeletal systems of other endangered frog species, allowing both versatile and high contrast images of anatomical structures without causing any significant damages to the living animal.


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Last Update: 2017-12-25