Jie WANG,Hongxing ZHANG,Feng XIE,et al.Genetic Bottlenecks of the Wild Chinese Giant Salamander in Karst Caves[J].Asian Herpetological Reserch(AHR),2017,8(3):174-183.[doi:10.16373/j.cnki.ahr.170038]
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Genetic Bottlenecks of the Wild Chinese Giant Salamander in Karst Caves
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2017 VoI.8 No.3
Page:
174-183
Research Field:
Publishing date:
2017-09-25

Info

Title:
Genetic Bottlenecks of the Wild Chinese Giant Salamander in Karst Caves
Author(s):
Jie WANG1* Hongxing ZHANG2 Feng XIE1 Gang WEI3 and Jianping JIANG1*
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China
2 Shaanxi Institute of Zoology, Xi’an 710032, Shaanxi, China
3 GuiyangUniversity, Guiyang 550005, Guizhou, China
Keywords:
limestone cave small population genetic drift Andrias davidianus subterranean habitat
PACS:
-
DOI:
10.16373/j.cnki.ahr.170038
Abstract:
The rarity of limestone cave species due to habitat degradation makes them of special interest in conservation biology. The wild Chinese Giant Salamander Andrias davidianus, an evolutionarily distinct and globally endangered species, are nearly all obligate into living in inaccessible mountain caves now. We detected that only 14–29 breeders, with the effective population size of 9–25 (5–44 of 95% confidence interval), were in each of three caves, through genotyping 20 microsatellite loci on larvae that were flushed out of caves. Both heterzygosity excess and M (ratios of allele number to allele size range) tests indicated severe genetic bottlenecks among populations. Both mitochondrial, with only one or two haplotypes of D-loop region (770–771bp) in each population, and nuclear genetic structure showed clear divergence between populations. Considering the long life history, small population size, and genetic differentiation of the Chinese Giant Salamander, putting an end to poaching and recovering the karst ecosystem instead of releasing may be the sole measures to save this severely threated species.

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Last Update: 2017-09-25