Lifu QIAN,Xiaonan SUN,Jiaqi LI,et al.A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from the Southern Dabie Mountains in Anhui Province[J].Asian Herpetological Reserch(AHR),2017,8(3):151-164.[doi:10.16373/j.cnki.ahr.170013]
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A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from the Southern Dabie Mountains in Anhui Province
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2017 VoI.8 No.3
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A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from the Southern Dabie Mountains in Anhui Province
Lifu QIAN1** Xiaonan SUN1** Jiaqi LI2 Weibo GUO2 Tao PAN1 Xing KANG1 Hui WANG1 Jianping JIANG3 Jun WU2* and Baowei ZHANG1*
1 Anhui Key Laboratory of Eco-engineering and Bio-technique, School of Life Sciences, Anhui University, Hefei 230601, Anhui, China
2 Nanjing Institute of Environmental Sciences under the Ministry of Environmental Protection, Nanjing 210042, Jiangsu, China
3 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China
new species Tylototriton anhuiensis sp. nov. southern Dabie Mountains taxonomy morphology molecular analyses
A new species of the genus Tylototriton is described, from Yuexi county, Anhui province, in the south of the Dabie Mountains. It is based on morphological and molecular analysis. The new species is identified as belonging to the Tylototriton asperrimus group and shares a number of similarities with T. wenxianensis, T. broadoridgus and T. dabienicus. The diagnostic characteristics of the new species are as follows: the head length is greater than the width of the head; bony ridges on the head are prominent and necked-in; the distal digit ends, ventral digits, peripheral area of the cloaca and the tail’s lower edge are orange. The result from the molecular analysis of the genus Tylototriton (including the type specimen of the new species) based on three mitochondrial genes (ND1, ND2 and CYTB) indicated that the new species was close to T. wenxianensis, T. dabienicus, and T. broadoridgus, but formed an independent clade. This result was consistent with the morphological analysis above, which supports the theory that the population distributed in the south of the Dabie Mountains, namely in from Yuexi county, Anhui province, represented a distinct species, Tylototriton anhuiensis sp. nov.


Akaike H. 1974. A new look at the statistical model identification. IEEE transactions on automatic control, 19(6): 716–723
Anderson J. 1871. Description of a new genus of newts from western Yunnan. In Proceedings of the zoological Society of London, Vol. 1871. 423–425
Bandelt H. J., Forster P., R?hl A. 1999. Median-joining networks for inferring intraspecific phylogenies. Mol Biol Evol, 16: 37–48
Bohme W., Schottler T., Nguyen T. Q., Kohler J. 2005. A new species of salamander, genus Tylototriton (Urodela: Salamandridae), from northern Vietnam. Salamandra-Bonn, 41(4): 215–220
Chen B. H. 1991. The amphibian and reptilian fauna of Anhui. Hefei: Anhui science and technology press, 39–41 (In Chinese)
Chen X. H., Wang X. W., Tao J. 2010. A new subspecies of genus Tylototriton from China (Caudata, Salamandridae). Acta Zootaxon Sinica, 35: 666–670 (In Chinese)
Clarke K. R., Gorley R. N. 2001. Primer v5. Primer-E Ltd
Drummond A. J., Suchard M. A., Xie D., Rambaut A. 2012 Bayesian phylogenetics with BEAUti and the BEAST 1.7. Mol Biol Evol, 29: 1969–1973
Dubois A., Raffa?lli J. 2009. A new ergotaxonomy of the family Salamandridae Goldfuss, 1820 (Amphibia, Urodela). Alytes, 26(1–4): 1–85
Eckert C., Samis K., Lougheed S. 2008. Genetic variation across species’ geographical ranges: the central-marginal hypothesis and beyond. Mol Ecol, 17(5): 1170–1188
Fang P. W., Chang M. L. Y. 1932. Notes on Tylototriton kweichouensis Unterstein with synopsis to species. Sinensia, 2(9): 111–122
Fei L. 1999. Atlas of amphibians of China. Zhengzhou: Henan science and technology press, 6–7 (In Chinese)
Fei L., Hu S. Q., Ye C. Y., Huang Y. Z. 2006. Fauna Sinica. Amphibia, Vol. I. Beijing: Seience Press, 1–471 (In Chinese)
Fei L., Ye C. Y., Jiang J. P. 2012. Colored Atlas of Chinese Amphibians and Their Distributions. Chengdu: Sichuan Publishing House of Science and Technology, 78–94 (In Chinese)
Fei L., Ye C. Y., Huan Y. Z., Jiang J. P., Xie F. 2005. An illustrated key to Chinese amphibians. Chengdu: Sichuan Publishing House of Science and Technology, 340 (In Chinese)
Fei L., Ye C. Y., Yang R. S. 1984. A new species and new subspecies of the genus Tylototriton (Caudata:Salamandridae ) Acta Zool Sin, 30(11), 85–9l (In Chinese)
Fei L., Ye C. Y., Huang Y. Z. 1990. The Key to Chinese Amphibian. Chongqing: Chongqing Branch of Science and Technology Literature Publishing House, 1–364 (In Chinese)
Fei L., Ye C. Y., Jiang J. P. 2010. Colored Atlas of Chinese Amphibians. Chengdu: Sichuan Publishing House of Science and Technology, 1–519 (In Chinese)
Frost D. 2016. Amphibian species of the world: an online reference. Version 6.0. American Museum of Natural History, New York, USA. Available at: (Assesses on Oct 11, 2016)
Hou M., Li P. P., Lv S. Q. 2012. Morphological research development of genus Tylototriton and primary confirmation of the status of four cryptic populations. J Huangshan Univ, 14: 61–65 (In Chinese)
Hu S. Q., Zhao E. M., Liu C. C. 1973. A survey of amphibians and reptiles Kweichow (= Guizhou) Province, including a herpetofaunal analysis. Acta Zool Sin, 19(2): 149–178 (In Chinese)
Hu S. Q., Ye C. Y., Fei L. 1977. Systematic Key to Amphibians of China. Beijing: Science Press, 1–93 (In Chinese)
Huang X., Pan T., Han D. M., Zhang L., Hou Y. X., Yu L., Zheng H. M., Zhang B. W. 2012. A new species of the genus Protobothrops (Squamata: Viperidae: Crotalinae) from the Dabie mountains, Anhui, China. Asian Herpetol Res, 3(3): 213–218
Jiang J. P., Chen X. H., Wang B. 2006. A new genus of family Ranidae from China - Yerana ( Ranidae : Dicroglossinae). Journal of Anhui Normal University (Natural Science), 29(5): 467–469 (In Chinese)
Khatiwada J. R., Wang B., Ghimire S., Vasudevan K., Paudel S., Jiang J. 2015. A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from Eastern Himalaya. Asian Herpetol Res, 6(4): 245–256
Le D. T., Nguyen T. T., Nishikawa K., Nguyen S. L. H., Pham A. V., Matsui M., Bernardes M., Nguyen T. Q. 2015. A new species of Tylototriton Anderson, 1871 (Amphibia: Salamandridae) from northern Indochina. Curr Herpetol, 34(1): 38–50
Librado P., Rozas J. 2009. DnaSP v5: A software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25:
Liu C. C. 1950. Amphibians of Western China, V02. Fieldiana: Zool Men, 102–110 (In Chinese)
Nishikawa K., Khonsue W., Pomchote P., Matsui M. 2013a. Two new species of Tylototriton from Thailand (Amphibia: Urodela: Salamandridae). Zootaxa, 3737(3): 261–279
Nishikawa K., Matsui M., Nguyen T. T. 2013b. A new species of Tylototriton from northern Vietnam (Amphibia: Urodela: Salamandridae). Curr Herpetol, 32: 34–49
Nishikawa K., Matsui M., Rao D.Q. 2014. A new species of Tylototriton (Amphibia: Urodela: Salamandridae) from central Myanmar. Nat Hist Bull Siam Soc, 60(1): 9–22
Nussbaum R. A., Brodie J. E. D., Datong Y. 1995. A taxonomic review of Tylototriton verrucosus Anderson (Amphibia: Caudata: Salamandridae). Herpetologica, 257–268
Nylander J. 2003. MrModeltest 1.0 b. a simplified version of David Posada’s “Modeltest 3.06”. Uppsala: Department of Systematic Zoology
Nylander, J. 2004. MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University
Pan T., Wang H., Hu C. C., Sun Z. L., Zhu X. X., Meng T., Meng X. X., Zhang B. W. 2015. Species delimitation in the genus Moschus (Ruminantia: Moschidae) and its high-plateau origin. PloS one, 10(8): e0134183
Pan T., Zhou W. L., Shi W. B., Zhao K., Chen J. Y., Wang W. G., Chu J., Pu F. G., Gu C. M., Zhang B. W. 2014. Species richness of amphibians and reptiles in Dabie Mountains, China. Chinese Journal of Zoology, 49(2): 195–206 (In Chinese)
Phimmachak S., Aowphol A., Stuart B. L. 2015. Morphological and molecular variation in Tylototriton (Caudata: Salamandridae) in Laos, with description of a new species. Zootaxa, 4006(2): 285–310
Rambaut A. 2009. FigTree version 1.3. 1. Computer program distributed by the author, website: http://treebioedacuk/software/figtree/ (accessed January 4, 2011)
Rambaut A., Drummond A. 2007. Tracer. Version 1.5
Rambaut A., Drummond A. 2010. TreeAnnotator version 1.6. 1
Ronquist F., Huelsenbeck J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1572–1574
Sambrook J., Fritsch E. F., Maniatis T. 1989. Molecular cloning. New York: Cold spring harbor laboratory press
Shen Y. H. 1989. A survey of the tailed amphibians in Hunan Province. Chin wildlife, (6): 77–84 (In Chinese)
Shen Y. H., Jiang J. P., Mo X. Y. 2012. A new species of the genus Tylototriton (Amphibia, Salamandridae) from Hunan, China. Asian Herpetol Res, 1(3): 21–30
Stamatakis A. 2006. RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. Bioinformatics, 22(21): 2688–2690
Stuart B., Phimmachak S., Niane S., Robichaud W. G. 2010. A new species in the Tylototriton asperrimus group (Caudata: Salamandridae) from central Laos. Zootaxa, 2650: 19–32
Tamura K., Peterson D., Stecher G., Nei M., Kumar S. 2011 MEGA5: Molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maxomum parsimony methods. Mol Biol Evol, 28: 2731–2739
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 25: 4876–4882
Tian W. S., Jiang Y. M. 1986. Identification manual of Chinese amphibians and reptiles. Beijing: Science Press, 1–164 (In Chinese)
Yang D. D., Jiang J. P., Shen Y. H., Fei D. B. 2014. A new species of the genus Tylototriton (Urodela: Salamandridae) from northeastern Hunan Province, China. Asian Herpetol Res, 5(1): 1–11
Zamudio K. R., Wieczorek A. M. 2007. Fine-scale spatial genetic structure and dispersal among spotted salamander (Ambystoma: maculatum) breeding populations. Mol Ecol, 16(2): 257–274
Zhang, P., Papenfuss, T. J., Wake, M. H., Qu, L., Wake, D. B. 2008. Phylogeny and biogeography of the family Salamandridae (Amphibia: Caudata) inferred from complete mitochondrial genomes. Mol Phylogenet Evol, 49(2): 586–597
Zhao T. Y., Rao D. Q., Liu N., Li B., Yuan S. Q. 2012. Molecular phylogeny analysis of Tylototriton verrucosus group and description of new Species. China Forest Sci, 41: 85–89 (In Chinese)


Last Update: 2017-09-25