Junwei LI,Zhencai YANG,et al.Effects of Dietary Vitamins A, B2, and B6 Supplementation on Growth and Feed Utilization of Juvenile Chinese Soft-shelled Turtle Pelodiscus sinensis according to an Orthogonal Array Experiment[J].Asian Herpetological Reserch(AHR),2016,7(4):278-286.[doi:10.16373/j.cnki.ahr.160005]
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Effects of Dietary Vitamins A, B2, and B6 Supplementation on Growth and Feed Utilization of Juvenile Chinese Soft-shelled Turtle Pelodiscus sinensis according to an Orthogonal Array Experiment
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Asian Herpetological Research[ISSN:1006-6977/CN:61-1281/TN]

2016 VoI.7 No.4
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Effects of Dietary Vitamins A, B2, and B6 Supplementation on Growth and Feed Utilization of Juvenile Chinese Soft-shelled Turtle Pelodiscus sinensis according to an Orthogonal Array Experiment
Junwei LI1 2 Zhencai YANG2* Xiaoling HAN2 Quansen XIE2 and Haiyan LIU2
1 Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization of Ministry of Agriculture of China, South China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Guangzhou 510300, China
2 College of Life Science, Hebei Normal University, Shijiazhuang 050024, Hebei Province, China
Pelodiscus sinensis vitamin growth performance digestion capacity orthogonal design
An orthogonal experimental design OA9 (33) was used to evaluate the effects of vitamins (A, B2, and B6) on the growth and digestive ability of the juvenile Chinese soft-shelled turtle, Pelodiscus sinensis (initial weight, 5.9±0.2 g). A total of 135 turtles were divided into 9 groups, which each included 15 individuals. The results revealed that vitamin A (VA) had the strongest impacts on the growth rate and feed utilization among the three vitamins; 35,000 IU kg-1 VA had optimal effects on the feeding intake and specific growth rate, and 20,000 IU kg-1 VA had optimal effects on protein digestibility and the feed conversion ratio. Vitamin B2 (VB2) was essential for regulating protein deposition and the energy efficiency for growth of the turtles; 120 mg kg-1 VB2 resulted in increased protein and energy deposition, and 180 mg kg-1 VB2 had greater beneficial effects on the growth rate. Vitamin B6 (VB6) had important effects on protein and feed efficiency; however, VB6 at an excessive level (120 mg kg-1) restricted turtle growth. Based on the above growth results, dietary supplementation of VA, VB2 and VB6 at levels of 35,000 IU kg-1, 180 mg kg-1 and 70 mg kg-1, respectively, were recommended for the juvenile soft-shelled turtle.


Albrektsen S., Waagbo R., Sandnes K. 1993. Tissue vitamin B concentrations and aspartate aminotransferase (Asp T) activity in Atlantic salmon (Salmo salar) fed graded dietary levels of vitamin B, Fik Dir Skr Ser Ernaring, 6: 21–34
Chen L. P., Huang C. H. 2014. Estimation of dietary vitamin A requirement of juvenile soft-shelled turtle, Pelodiscus sinensis. Aquac Nutr, Doi: 10.1111/anu. 12172
Deng D. F., Wilson R. P. 2003. Dierary riboflavin requirement of juvenile sunshine bass (Morone chrysops♀×Morone saxatilis♂). Aquaculture, 218: 695–701
Feng L., He W., Jiang J., Liu Y., Zhou X. Q. 2010. Effects of dietary pyridoxine on disease resistance, immune responses and intestinal microflora in juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Nutr, 16: 254–261
Fisheries Department of Agriculture Ministry of China. 2012. China Fisheries Yearbook. Beijing: China Agriculture Press (In Chinese)
Funkenstein B. 2001. Developmental expression, tissue distribution and hormonal regulation of fish (Sparusaurata) serum retinol-binding protein. Comp Biochem Phys, 129: 613–622
Giri N. A., Teshima S. I., Kanazawa A. 1997. Effects of dietary pyridoxine and protein levels on growth, vitamin B6 content, and free amino acid profile of juvenile Penaeus japonicus. Aquaculture, 157: 263–275
Halver J. E. 1989. The vitamins. In Halver J. E. (Ed.), Fish Nutrition. New York: Academic Press, 32–102
Halver J. E. 2003. The vitamins. In Halver J. E. (Ed.), Fish Nutrition, 3rd Edition. New York: Academic Press, 61–141
He W., Zhou X. Q., Feng L., Jiang J., Liu Y. 2009. Dietary pyridoxine requirement of juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Nutr, 15: 402–408
Hemre G. I., Deng D. F., Wilson R. P., Berntssen M. H. G. 2004. VitaminA metabolism and early biological responses in juvenile sunshine bass (Moronechrysop×M.saxatilis) fed graded levels of vitaminA. Aquaculture, 235: 645–665
Hernandez L. H. H., Teshima S. I., Ishikawa M., Alam S., Koshio S., Tanaka Y. 2005. Dietary vitamin A requirements of juvenile Japanese flounder Paralichthys olivaceus. Aquac Nutr, 11: 3–9
Hilton J. W. 1989. The interaction of vitamins, minerals and diet composition in the diet of fish. Aquaculture, 79: 223–244
Hou J. L., Jia Y. J., Yang Z. C., Li Y. J., Cheng F. X., Li D., Ji F. S. 2013. Effects of Taurine Supplementation on Growth Performance and Antioxidative Capacity of Chinese Soft-shelled Turtles, Pelodiscus sinensis, Fed a Diet of Low Fish Meal Content. J World Aquac Soc, 44: 786–794
Huang C., Lin W., Wu S. 2003. Effect of dietary calcium and phosphorus supplementation in fish meal-based diets on the growth of soft-shelled turtle Pelodiscus sinensis (Wiegmann). Aquac Res, 34: 843-848
Huang C., Lin W. 2004. Effects of dietary vitamin E level on growth and tissue lipid peroxidation of soft-shelled turtle, Pelodiscus sinensis (Wiegmann). Aquac Res, 35: 948-954
Kavita P. P., David H. B. 1996. Supplemtantal iron, copper, zinc, ascorbate, caffeine and chlortetracycline do not affect riboflavin utilization in the chick. Nutr Res, 16: 1943–1952
Lahov E., Regelson W. 1996. Antibacterial and immuno-stimulating casein-de-rived substances from milk, casecidin, isracidin peptides. Food Chem Toxicol, 34: 131–145
Li E. C., Yu N., Chen L. Q., Zeng C., Liu L. H., Qin J. G. 2010. Dietary Vitamin B6 Requirement of the Pacific White Shrimp, Litopenaeus vannamei, at Low Salinity. J World Aquac Soc, 41: 756–763
Li W., Zhou X. Q., Feng L., Liu Y., Jiang J. 2010. Effect of dietary riboflavin on growth, feed utilization, body composition and intestinal enzyme activities of juvenile Jian carp (Cyprinus carpio var. Jian). Aquac Nutr, 16: 137–143
Lin S. M., Zeng D. M., Ye Y. S., Luo L. 2003. A study on vitamin B2, B6, niacin and pantothenic acid requirements of allogenetic crucian carps. Chin J Anim Nutr, 15: 43–47
Masumoto T. 2002. Yellowtail, Seriola quinqueradiata. In Webster C. D., Lim C. (Eds.), Nutrient Requirement and Feeding of Finfish for Aquaculture. New York: CABI Publishing, 131–146
Mohamed J. S., Sivaram V., Christopher R., Marian M. P., Murugardass S., Hussain M. R. 2003. Dietary vitamin A requirement of juvenile greasy grouper (Epinephelus tauvina). Aquaculture, 219: 693–701
Montgomery D. C. 1991. Design and Analysis of Experiments, 3rd Edition. New York: John Wiley and Sons, 649
Moren M., Opstad I., Bemtssen M. H. G., Zambonino I. J. L., Hamre K. 2004. An optimum level of vitamin A supplements for Atlantichalibut (Hippoglossus hippoglossus L.) juveniles. Aquaculture, 235: 587–599
Nuangsaeng B., Boonyaratapalin M. 2001. Protein requirement of juvenile soft-shelled turtle Trionyx sinensis Wiegmann. Aquaculture Research, 32: 106-111
NRC (National Research Council). 1993. Nutrient Requirements of Fish, National Academy Press, Washington, DC., 114
Olson J. A. 1991. Vitamin A. In Machin, L. (Ed.), The Handbook of vitamins. New York: Marcel Dekker, 1–59
Pu L. J., Niu C. J. 2013. Molecular cloning and characteristics of catalase cDNA from Chinese soft-shelled turtle (Pelodiscus sinensis). Asian Herpetol Res, 4(2): 90–99
Reham K. N., Jennifer M. C., Malcolm R. B., Barbara F. N., Stephen C. B. 2013. The effects of dietary vitamin A in rotifers on the performance and skeletal abnormality of striped trumpeter Latris lineata larvae and post larvae. Aquaculture, 404–405: 105–115
Rong C. K., Zhen R. L., Yue B. Y., Liang S. X. 1996. Studies on the nutritional requirements of fat-solube vitamins A, D3, E K3 for shrimp Penaeus Chinensis. J Tianjin Agric Coll, 3: 1– 6
Roy R. K. 1990. A primer on the Taguchi Method. Van Nostrand Reinhold, 7–9
Serrini G., Zhang Z., Wilson R. P. 1996. Dietary riboflavin requirement of fingerling channel catfish (Ictalurus punctatus). Aquaculture, 139: 285–290
Shiau S. Y., Chen Y. 2000. Estimation of the dietary vitamin A requirement of juvenile grass Shrim P. Penaeusmondon. Nutrition, 130: 90–94
Souto M., Saavedra M., Ferreira P. P., Herrero C. 2008. Riboflavin enrichment throughout the food chain from the marine microalgae Tetraselmis suecica to the rotifer Brachionus plicatilis and to White Sea Bream (Diplodus sargus) and Gilthead Sea bream (Sparus aurata) larvae. Aquaculture, 283: 128–133
Stéphanie F. D., Emilie L., Anne S., Jeannine B., José-Luis Z. I., Sadasivam J. K. 2010. Effects of dietary vitamin A on broodstock performance, egg quality, early growth and retinoid nuclear receptor expression in rainbow trout (Oncorhynchus mykiss). Aquaculture, 303: 40–49
Tan Q. S., He R. G., Xie S. Q., Xie C. X., Zhang S. P. 2007. Effect of Dietary Supplementation of Vitamins A, D3, E, and C on Yearling Rice Field Eel, Monopterus albus: Serum Indices, Gonad Development, and Metabolism of Calcium and Phosphorus. J World Aquac Soc, 38: 146–153
Wang A., Shan A. S. 2007. Vitamin modern animal agricultural production. Beijing: Science press, 126
Xie Q. S., Yang Z. C., Li J. W., Li Y. J. 2012. Effect of protein restriction with subsequent re-alimentation on compensatory growth of juvenile soft-shelled turtles (Pelodiscus sinensis). Aquac Int, 20: 19–27
Xu Z. C., Liu T. B., Li A. J. 1995. Studies on the requirement for riboflavin nicotinamide and pyridoxine in the prawn Penaeus Chinensis. J Fish China, 19: 97–104
Yan L. L., Zhang G. F., Liu Q. G., Li J. L. 2009. Optimization of culturing the freshwater pearl mussels, Hyriopsis cumingii with filter feeding Chinese carps (bighead carp and silver carp) by orthogonal array design. Aquaculture, 292: 60–66
Yutaka H., Du S. J., Shuichi S., Tomonari K., Hiroshi F., Toshio T. 2011. Analysis of the mechanism of skeletal deformity in fish larvae using a vitamin A-induced bone deformity model. Aquaculture, 315: 26–33
Zheng S., Jiang F. 2004. Experiment Design and Data Processing. Beijing: China architecture and industry publishing company, 60–90
Zhong W. R., Zhang S. H. 2001. Studies on the Requirements of Lateclabrux japonicus for Vitamins at Various Growth Stages. J Zhejiang Ocean Univ (Nat Sci), 20: 98–102
Zhou X. Q., Niu C. J., Sun R. Y. 2004. Effects of the combination of vitamin C and E on non-specific immune function in juvenile soft-shelled turtle Trionyx Sinensis. Acta Hydrobiol Sinica, 28: 356–360


Last Update: 2016-12-25