Baorong GENG,Lingling ZHANG,Yun JIANG,et al.Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay[J].Asian Herpetological Reserch(AHR),2016,7(4):251-257.[doi:10.16373/j.cnki.ahr.150058]
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Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay
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Asian Herpetological Research[ISSN:1006-6977/CN:61-1281/TN]

2016 VoI.7 No.4
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Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay
Baorong GENG* Lingling ZHANG Yun JIANG Xiuping HUANG and Jinmei DAI
College of Life Sciences, Fujian Normal University, Fuzhou 350117, Fujian, China
Polypedates megacephalus tadpole avermectins abamectin ivermectin emamectin benzoate DNA damage comet assay
Avermectins are a new class of macrocyclic lactones derived from mycelia of the soil actinomycete, and are used as effective agricultural pesticides and antiparasitic agents. However, run-off from crops treated with avermectins may contaminate various bodies of water, and accumulated to certain concentrations to impact the development of aquatic animals. Here, we tested the genotoxicity of three avermectins (abamectin, ABM; ivermectin, IVM; and emamectin benzoate, EMB) on Polypedates megacephalus tadpoles by the alkaline single-cell gel electrophoresis assay. Tadpoles were treated for 48 h in the laboratory with different concentrations of these three agents, 0.006, 0.012, 0.018, 0.024, 0.030 mg/L for ABM, 0.003, 0.006, 0.009, 0.012, 0.015 mg/L for IVM and 0.04, 0.06, 0.08, 0.10, 0.12 mg/L for EMB, and then measured their DNA damage by the Comet assay tail factor %. The concentrations of resulted in highly significant increases in DNA damage of the tadpoles were found above the concentration threshold of 0.012 mg/L ABM, 0.003 mg/L IVM and 0.06 mg/L EMB and linear correlations between the intensity of DNA damage and the concentrations of these three avermectins. Our results showed clearly that avermectins caused dose dependent DNA damage on amphibian tadpoles, and there might be a control on the misuse of avermectins.


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Last Update: 2016-12-25