Hebbar PRITI,Chandrakanth Rukkappa NAIK,Kadaba Shamanna SESHADRI,et al.A New Species of Euphlyctis (Amphibia, Anura, Dicroglossidae) from the West Coastal Plains of India[J].Asian Herpetological Reserch(AHR),2016,7(4):229-241.[doi:10.16373/j.cnki.ahr.160020]
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A New Species of Euphlyctis (Amphibia, Anura, Dicroglossidae) from the West Coastal Plains of India
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Asian Herpetological Research[ISSN:1006-6977/CN:61-1281/TN]

2016 VoI.7 No.4
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A New Species of Euphlyctis (Amphibia, Anura, Dicroglossidae) from the West Coastal Plains of India
Hebbar PRITI12 Chandrakanth Rukkappa NAIK3 Kadaba Shamanna SESHADRI4 Ramit SINGAL5Madhava Kulkarni VIDISHA6 Gudasalmani RAVIKANTH1 and Kotambylu Vasudeva GURURAJA67*
1 Ashoka Trust for Research in Ecology and the Environment (ATREE), Royal Enclave, Sriramapura, Jakkur (P.O), Bangalore 560054, India
2 Manipal University, Manipal 576104, India
3 Wildlife Range, Kumbaravada, Nujji Section, Dandeli Anshi Tiger Reserve, Joida Taluk, 581187, India
4 Department of Biological Sciences, National University of Singapore, 14 Science Drive 4, Block S3, Singapore
5 Independent Researcher, B-14, Law Apartments, Karkardooma, Delhi 110092, India
6 Gubbi Labs LLP, Science and Media Center, WS-5, I Floor, Entrepreneurship Center, Indian Institute of Science Campus, Bengaluru 560012, India
7 Srishti Institute of Art, Design and Technology, N4, Yelahanka New Town, Bengaluru 560064, India
Karaavali skittering frog citizen science molecular identification agriculture cryptic species
The genus Euphlyctis is widely distributed across Southwestern Arabian Peninsula into parts of Southeast Asia. Five of the seven known Euphlyctis species are found within the Indian subcontinent. Here, we describe a new species, Euphlyctis karaavali sp. nov. from South-west coast of India, which was discovered during surveys engaging citizens. This species was identified to be distinct based on molecular and morphological evidence. We provide a detailed description of this species along with its call description and compare it with closest congeners. Previous studies in the region had identified this species as E. hexadactylus but suggested the possibility of it being cryptic. Genetically E. karaavali sp. nov. is distinct from E. hexadactylus with a genetic divergence of 9.2% (12S and 16S) and shows a high divergence with E. kalasgramensis and E. ehrenbergii (13.04% each). Our findings are discussed in the context of cryptic species discovery, citizen engagement in scientific progress and conservation measures while suggesting future directions.


Alam M. S., Igawa T., Khan M. M., Islam M. M., Kuramoto M., Matsui M., Kurabayashi A., Sumida M. 2008. Genetic divergence and evolutionary relationships in six species of genera Hoplobatrachus and Euphlyctis (Amphibia: Anura) from Bangladesh and other Asian countries revealed by mitochondrial gene sequences. Mol Phylogenet Evol, 48(2): 515–527
Boulenger G. A. 1920. A monograph of the South Asian, Papuan, Melanesian and Australian frogs of the genus Rana. India: Zoological Survey of India
Das A., Krishnaswamy J., Bawa K. S., Kiran M. C., Srinivas V., Kumar N. S., Karanth K. U. 2006. Prioritisation of conservation areas in the Western Ghats, India. Biol Conserv, 133(1): 16–31
Frost D. R. 2016. Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, USA. Available: http://researchamnhorg/herpetology/amphibia/indexhtml
Gururaja K. V., Dinesh K. P., Priti H., Ravikanth G. 2014. Mud-packing frog: a novel breeding behaviour and parental care in a stream dwelling new species of Nyctibatrachus (Amphibia, Anura, Nyctibatrachidae). Zootaxa, 3796: 33–61
Hartop E. A., Brown B. V., Disney R. H. 2015. Opportunity in our Ignorance: Urban Biodiversity Study Reveals 30 New Species and One New Nearctic Record for Megaselia (Diptera: Phoridae) in Los Angeles (California, USA). Zootaxa, 3941: 451–484
Howlader M. S., Nair A., Gopalan S. V., Merila J. 2015. A new species of Euphlyctis (Anura: Dicroglossidae) from Barisal, Bangladesh. PLoS ONE, 10(2): e0116666
Joshy S. H., Alam M. S., Kurabayashi A., Sumida M., Kuramoto M. 2009. Two new species of the genus Euphlyctis (Anura: Ranidae) from Southwestern India as revealed by molecular and morphological comparisons. Alytes, 26(1-4): 97–116
Katoh K., Misawa K., Kuma K. I., Miyata T. 2002. MAFFT: a novel method for rapid multiple sequence alignment based on fast Fourier transform. Nucleic acids res, 30(14): 3059–3066
Khan M. S. 1997. A new subspecies of common skittering frog Euphlyctis cyanophlyctis (Schneider, 1799) from Balochistan, Pakistan. Pakistan J Zool, 29: 107–112
Kok P. J., Kalamandeen M. 2008. Introduction to the taxonomy of the amphibians of Kaieteur National Park, Guyana. Belgian Development Cooperation
Kosuch J., Vences M., Dubois A., Ohler A., Bohme W. 2001. Out of Asia: Mitochondrial DNA evidence for an oriental origin of tiger frogs, genus Hoplobatrachus. Mol Phylogenet Evol, 21: 398–407
Kurabayashi A., Kuramoto M., Joshy H., Sumida M. 2005. Molecular Phylogeny of the Ranid Frogs from Southwest India based on the Mitochondrial Ribosomal RNA Gene Sequences. Zool Sci, 22: 525–534
Meenakshi K., Sujith VG., Sanil G. 2009. DNA barcoding of some amphibians of Western Ghats. 3rd International Barcode of life Conference (In Press)
Palumbi S., Martin A., Romano S., McMillan W., Stice L., Grabowski G. 2002. The simple fool’s guide to PCR. Honolulu USA
Posada D. 2008. jModelTest: phylogenetic model averaging. Mol Biol Evol, 25: 1253–1256
Priti H., Roshmi R. S., Ramya B., Sudhira H. S., Ravikanth G., Aravind N. A., Gururaja K. V. 2016. Integrative Taxonomic Approach for Describing a New Cryptic Species of Bush Frog (Raorchestes: Anura: Rhacophoridae) from the Western Ghats, India. PLoS ONE, 11(3): e0149382
Ronquist F., Teslenko M., van der Mark P., Ayres D. L., Darling A., H?hna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. 2012. MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol, 61(3): 539–542
Rosenzweig M. L. 2003. Reconciliation ecology and the future of species diversity. Oryx, 37(02): 194–205
Roy D., Elepfandt A. 1993. Bioacoustic analysis of frogs from northeast India. J Biosci, 18(3): 381–393
Seshadri K.S., Ramit S., Priti H., Ravikanth G., Vidisha M.K., Saurabh S., Pratik M., Gururaja K.V. 2016. Microhyla laterite sp. nov., A New Species of Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from a Laterite Rock Formation in South West India. PLoS ONE, 11(3): e0149727. doi:10.1371/journal.pone.0149727
Silvestro D., Michalak I. 2012. raxmlGUI: a graphical front-end for RAxML. Org Divers Evol, 12(4): 335–337
Simon C., Friati F., Beckenbach A., Crespi B., Liu H., Flook P. 1994. Evolution, weighting and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann Entomol Soc Am ,87(6): 651–701
Tamura K., Petersonm D., Peterson N., Stecher G., Nei M., Kumar S. 2011. MEGA5: Molecular Evolutionary Genetics Analysis using Maximum Likelihood, Evolutionary Distance, and Maximum Parsimony Methods. Mol Biol Evol, 28: 2731–2739
Vences M. 2000. Phylogenetic studies of ranoid frogs (Amphibia: Anura), with a discussion of the origin and evolution of the vertebrate clades of Madagascar. PhD Thesis submitted to Universitaet Bonn
Vences M., Nagy Z., T, Sonet G., Verheyen E. 2012. DNA barcoding Amphibians and reptiles. Kress, WJ & Erickson, DL (eds) DNA Barcodes: Methods and Protocols, Methods in Molecular Biology Springer Science+Business Media, LLC 2012: 79–108


Last Update: 2016-12-25