Qiyu LI,Tingting ZOU,Wenqi TANG,et al.Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia[J].Asian Herpetological Research(AHR),2022,13(1):23-33.[doi:10.16373/j.cnki.ahr.200125]
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Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2022 VoI.13 No.1
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Offspring Sex Is Not Determined by Gestation Temperature in a Viviparous Lizard (Eremias multiocellata) from the Desert Steppe of Inner Mongolia
Qiyu LI12 Tingting ZOU12 Wenqi TANG12 Yang WANG3 Weiguo DU1 and Xifeng WANG1*
1 Key Laboratory of Animal Ecology and Conservation Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing 100101, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
3 Key Laboratory of Animal Physiology, Biochemistry and Molecular Biology of Hebei Province, College of Life Sciences, Hebei Normal University, Shijiazhuang 050024, Hebei, China
geographic variation maternal thermal environment reptile sex chromosome sex determination sex ratio sex reversal
Sex-determining systems show a striking diversity not only among species, but also among populations. In reptiles, sex-determination is a continuum, from temperature-dependent sex determination (TSD) to genetic sex determination (GSD). The multi-ocellated racerunner (Eremias multiocellata) is reported to be a cryptic ZZ/ZW chromosomal TSD species, with male-biased sex ratios at high temperatures in two Gansu populations. However, the generality of the sex-determining pattern in different populations of this species remains unclear. To investigate the mode of sex determination in a population of E. multiocellata from the desert steppe of Inner Mongolia, we first identified sex chromosomes via comparative genomic hybridization (CGH). We then conducted a thermal manipulation experiment to determine the effect of gestation temperature on offspring sex ratios. From the CGH studies we found that lizards from the Inner Mongolia population possessed ZZ/ZW sex chromosomes. However, our thermal manipulation experiment showed that gestation temperature did not affect the sex ratio of neonates in this population. In combination, these results rule out TSD in the Inner Mongolia population of E. multiocellata, and suggest that there is widespread geographic variation in the sex-determining system of this species.


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Last Update: 2022-03-25