Hongliang LU,Yingchao HU,Changqing XU,et al.Developmental and Liver Metabolite Changes Induced by TPhP Exposure in Brown Frog (Rana zhenhaiensis) Tadpoles[J].Asian Herpetological Research(AHR),2021,12(1):135-142.[doi:10.16373/j.cnki.ahr.200044]
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Developmental and Liver Metabolite Changes Induced by TPhP Exposure in Brown Frog (Rana zhenhaiensis) Tadpoles
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2021 VoI.12 No.1
Page:
135-142
Research Field:
Publishing date:
2021-03-25

Info

Title:
Developmental and Liver Metabolite Changes Induced by TPhP Exposure in Brown Frog (Rana zhenhaiensis) Tadpoles
Author(s):
Hongliang LU1;  Yingchao HU1;  Changqing XU2;  Wei DANG1;  Zhihua LIN3*
1Key Laboratory of Hangzhou City for Ecosystem Protection and Restoration, School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 311121, Zhejiang, China
2 Medical School, Hangzhou Normal University, Hangzhou 311121, Zhejiang, China
3 College of Ecology, Lishui University, Lishui 323000, Zhejiang, China
Keywords:
larval development;  liver metabolite;  triphenyl phosphate exposure;  Zhenhai brown frog
PACS:
-
DOI:
10.16373/j.cnki.ahr.200044
Abstract:
As an organophosphorus compound that frequently detected in water samples, triphenyl phosphate (TPhP) has been showed to have multiple toxicological effects on aquatic species. However, no attention has been paid to its potential impact on non-model amphibian species. Here, tadpoles of the Zhenhai brown frog (Rana zhenhaiensis) were exposed to different concentrations of TPhP (0, 0.02 and 0.1 mg/L) throughout the developmental period to assess physiological and metabolic impacts of TPhP exposure on amphibian larvae. After 30-day TPhP exposure, the developmental stage of tadpoles from the high-concentration treatment appeared to be more advanced than that from the other two treatments, but other measured traits (including body size, tail length and liver weight) did not differ among treatments. Metabolite profiles in tadpole livers based on liquid chromatography-mass spectrometry (LC-MS) revealed a distinct metabolic disorder in exposed animals. Specifically, significant changes in various hepatic amino acids (such as glutamine, glutamate, valine and leucine) were observed. Overall, our results indicated that chronic TPhP exposure potentially caused developmental and hepatic physiological changes in R. zhenhaiensis tadpoles, although its impact on tadpole growth appeared to be minor.

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Last Update: 2021-03-25