Janak Raj KHATIWADA,Guocheng SHU,Bin WANG,et al.Description of a New Species of Amolops Cope, 1865 (Amphibia: Ranidae) from Nepal and Nomenclatural Validation of Amolops nepalicus Yang, 1991[J].Asian Herpetological Research(AHR),2020,11(2):71-94.[doi:10.16373/j.cnki.ahr.190052]
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Description of a New Species of Amolops Cope, 1865 (Amphibia: Ranidae) from Nepal and Nomenclatural Validation of Amolops nepalicus Yang, 1991
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2020 VoI.11 No.2
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Description of a New Species of Amolops Cope, 1865 (Amphibia: Ranidae) from Nepal and Nomenclatural Validation of Amolops nepalicus Yang, 1991
Janak Raj KHATIWADA Guocheng SHU Bin WANG Tian ZHAO Feng XIE Jianping JIANG*
CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization & Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
A. marmoratus group mahabharat range molecular phylogeny taxonomy
A new species, Amolops mahabharatensis sp. nov., is described from Mahabharat range (middle mountain) of Nepal. The new species is a member of the A. marmoratus group and is distinguished from its congeners using morphological and molecular data. The new species is diagnosed by having numerous warts on dorsum and flanks, dorsal body greyish olive-brown/light brown, presence of vomerine teeth series, presence of a pair of subgular vocal sacs in males and tadpole with III:5+5/1+1:II – labial tooth row. In addition, evidenced by the phylogenetic and morphological analyses in this study, Amolops nepalicus is shown to be a valid species. A molecular phylogenetic analysis based on 16S ribosomal gene for six members of the A. marmoratus group suggested that the new species is sister to a clade containing A. nepalicus and A. panhai. The uncorrected genetic divergences between the new species and its closest congeners A. nepalicus, A. panhai and A. marmoratus based on 16S rRNA gene were 10.5%, 11.6%, and 8.6% respectively. The new species showed wide distribution in the Mahabharat range of Nepal.


Altig R., McDiarmid R. W. 1999. Body plan: development and morphology. In: R W McDiarmid and R Altig (eds.) Tadpoles: the biology of anuran larvae. The Universiti of Chicago Press, Chicago. p 418
Anderson J. 1871. A list of the reptilian accession to the Indian Museum, Calcutta, from 1865 to 1870, with a description of some new species. J Asiatic Soc Ben, 40(2): 12–39
Annandale, N. 1912. Zoological results of the Abor Expedition, 1911–1912. I. Amphi Rec Ind Mus, 8: 7–36
Bain R. H., Lathrop A., Murphy R. W., Orlov N. L., Cuc H. T. 2003. Cryptic species of a cascade frog from Southeast Asia: taxonomic revisions and descriptions of six new species. Am Mus Novit, 2003(3417): 1–60
Bain R. H., Truong N. Q. 2004. Herpetofaunal diversity of Ha Giang Province in northeastern Vietnam, with descriptions of two new species. Am Mus Novit, 2004: 1–42
Bain R. H., Stuart B. L., Orlov N. L. 2006. Three new Indochinese species of cascade frogs (Amphibia: Ranidae) allied to Rana archotaphus. Copeia, 2006: 43–59
Biju S., Mahony S., Kamei R. G. 2010. Description of two new species of torrent frog, Amolops Cope (Anura: Ranidae) from a degrading forest in the northeast Indian state of Nagaland. Zootaxa, 2408(1): 31–66
Biju S., Mahony S., Kamei R. G. 2010. Description of two new species of torrent frog, Amolops Cope (Anura: Ranidae) from a degrading forest in the northeast Indian state of Nagaland. Zootaxa, 2408: 31–66
Blyth E. 1855. Report for October meeting. J Asiatic Soc Ben, 24(7): 711–723
Bossuyt F., Milinkovitch M. C. 2000. Convergent adaptive radiations in Madagascan and Asian ranid frogs reveal covariation between larval and adult traits. P Natl Acad Sci, 97(12): 6585–6590
Boulenger G. A. 1888. Descriptions of two new Indian species of Rana. Annals Mag Nat Hist, 6: 506–508
Boulenger G. A. 1899. Descriptions of new Batrachians in the collection of the British Museum (Natural History). J Nat Hist, 3: 273–277
Boulenger G. A. 1900. On the reptiles, batrachians, and fishes collected by the late Mr. John Whitehead in the interior of Hainan. Proc Zool Soc Lond, 1899: 956–962
Boulenger G. A. 1920. A monograph of the South Asian, Papuan, Melanesian, and Australian frogs of the genus Rana. Rec Ind Mu,s 20: 1–126
Chan K. O., Abraham R. K., Grismer J. L., Grismer L. L. 2018. Elevational size variation and two new species of torrent frogs from Peninsular Malaysia (Anura: Ranidae: Amolops Cope). Zootaxa, 4434(2): 250–264
Cope E. D. 1865. Sketch of the primary groups of Batrachia salientia. Nat Hist Rev, 5: 97–120
Das I. 1998. A New Species of Rana from the Terai of Nepal. J Herpetol, 32(2): 223–229
Dever J. A., Fuiten A. M., Konu ?., Wilkinson J. A. 2012. Cryptic torrent frogs of Myanmar: an examination of the Amolops marmoratus species complex with the resurrection of Amolops afghanus and the identification of a new species. Copeia, 2012(1): 57–76
Dinesh K., Radhakrishnan C., Gururaja K., Bhatta G. 2009. An annotated checklist of amphibian of India with some insights into the patterns of species discoveries, dstribution and endemism. Dehra Dun, India: Zoological Survey of India. p 153
Dubois A. 2004. Amphibians of Nepal: a few words of caution. Alytes, 21(4): 174–180
Dubois A., Matsui M. 1983. A new species of frog (genus Rana, subgenus Paa) from western Nepal (Amphibia: Anura). Copeia, 1983(4): 895–901
Fei L., Ye C., Jiang J. 2012. Colored Atlas of Chinese Amphibians and Their Distributions. Sichuan Publishing House of Science and Technology, Chengdu, China
Fei L., Ye C.Y., Wang Y.F., Jiang K. 2017. A new species of the genus Amolops (Anura: Ranidae) from high–altitude Sichuan, southwestern China, with a discussion on the taxonomic status of Amolops kangtingensis. Zool Res, 38(3): 138–145
Frost D. R. 2019. Amphibian Species of the World: an Online Reference. Version 6.0. Retrieved from http://research.amnh.org/herpetology/amphibia/index.html. Accessed 2019.09.04 2019
Günther, A. 1858. Catalogue of the Batrachia Salientia in the Collection of the British Museum. Taylor and Francis, London
Günther A. 1875. Second report on collections of Indian reptiles obtained by the British Museum. Pro Zool Soc Lon, 43(1): 224–234
Inger R. F. 1966. The Amphibia of Borneo. Fieldiana Zool, 52: 1–402
Inger R. F., Kottelat M. 1998. A new species of ranid frog from Laos. Raff Bull Zool, 46: 29–34
Inger R. F., Orlov N. L., Darevsky I. S. 1999. Frogs of Vietnam: a report on new collections. Field Zool New Ser, 92: 1–46
Inger R., Chanard T. 1997. A new species of ranid frog from Thailand, with comments on Rana livida (Blyth). Nat Hist Bull Siam Soc, 45: 65–70
Jiang K., Wang K., Yan F., Xie J., Zou D., Liu W., Jiang J., Li C., Che J. 2016. A new species of the genus Amolops (Amphibia: Ranidae) from southeastern Tibet, China. Zool Res, 37(1): 31–40
Jiang, Y. 1983. A new species of the genus Staurois (Ranidae) —Staurois viridimaculatus. Acta Herpetol Sinica, 2:71.
Khatiwada J. R., Haugaasen T. 2015. Anuran species richness and abundance along an elevational gradient in Chitwan, Nepal. Zool Ecol, 25(2): 110–119
Khatiwada J. R., Wang B., Ghimire S., Vasudevan K., Paudel S., Jiang J. 2015. A New Species of the Genus Tylototriton (Amphibia: Urodela: Salamandridae) from Eastern Himalaya. Asian Herpetol Res, 6(4): 245–256
Khatiwada J. R., Shu G. C., Wang S. H., Thapa A., Wang B., Jiang J. 2017. A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal. Zootaxa, 4254(2): 221–239
Khatiwada J. R., Shu G., Subedi T. R., Wang B., Ohler A., Canatella D. C., Xie F., Jiang J. P. 2019a. A New Species of Megophryid Frog of the Genus Scutiger from Kangchenjunga Conservation Area, Eastern Nepal. Asian Herpetol Res, 10(3): 139–157
Khatiwada J. R., Zhao T., Chen Y., Wang B., Xie F., Cannatella D. C., Jiang J. 2019b. Amphibian community structure along elevation gradients in eastern Nepal Himalaya. BMC Ecol, 19(1): 19
Kripalani, M. B. 1961. Zoological Results of the Indian Cho-Oyu Expedition (1958) in Nepal. Part 5 – Amphibia. Rec Indian Mus, 59: 241-244
Kumar S., Stecher G., Tamura K. 2016. MEGA7: Molecular Evolutionary Genetics Analysis version 7.0 for bigger datasets. Mol Biol Evol, 33(7): 1870–1874
Liu C. C. 1945. New frogs from west China. J West China Bor Res Soc, 15: 28–43
Liu C. C. 1950. Amphibians of western China. Field Zool Memo, 2: 1–397
Liu C. C., Hu S. Q. 1975. Report on three new species of Amphibia from Fujian Province. Acta Zool Sinica, 21: 265–271
Liu C., Hu S. 1961. Tailless amphibians of China Science Press, Beijing, China [In Chinese]
Liu W., Yang D. 2000. A new species of Amolops (Anura: Ranidae) from Yunnan, China, with a discussion of karyological diversity in Amolops. Herpetologica, 56: 231–238
Liu W., Yang D., Ferraris C., Matsui M., Price A. 2000. Amolops bellulus: A new species of stream–breeding frog from western Yunnan, China (Anura: Ranidae). Copeia, 2000: 536–541
Lyu Z. T., Wu J., Wang J., Sung Y. H., Liu Z. Y., Zeng Z. C., Wang X., Li Y. Y., Wang Y. Y. 2018. A new species of Amolops (Anura: Ranidae) from southwestern Guangdong, China. Zootaxa, 4418: 562–576
Lyu Z. T., Huang L. S., Wang J., Li Y. Q., Chen H. H., Qi S., Wang Y. Y. 2019a. Description of two cryptic species of the Amolops ricketti group (Anura, Ranidae) from southeastern China. ZooKeys, 812: 133–156
Lyu Z. T., Zeng Z. C., Wan H., Yang J. H., Li Y. L., Pang H., Wang Y. Y. 2019b. A new species of Amolops (Anura: Ranidae) from China, with taxonomic comments on A. liangshanensis and Chinese populations of A. marmoratus. Zootaxa, 4609(2): 247–268
Mahony S., Kamei R. G., Teeling E. C., Biju S. 2018. Cryptic diversity within the Megophrys major species group (Amphibia: Megophryidae) of the Asian Horned Frogs: Phylogenetic perspectives and a taxonomic revision of South Asian taxa, with descriptions of four new species. Zootaxa, 4523(1): 1–96
Matsui M., Nabhitabhata J. 2006. A new species of Amolops from Thailand (Amphibia, Anura, Ranidae). Zool Sci, 23: 727–732
Matsui M., Shimada, T., Liu W. Z., Maryati M., Khonsue W., Orlov N. 2006. Phylogenetic relationships of Oriental torrent frogs in the genus Amolops and its allies (Amphibia, Anura, Ranidae). Mol Phylogenet Evol, 38(3): 659–666
Nanhoe L. M., Ouboter P. E. 1987. The distribution of reptiles and amphibians in the Annapurna–Dhaulagiri region (Nepal). Zool. Verh. Leiden, 240: 1–100
Nidup T., Gyeltshen D., Penjor S. D., Pearch M. J. 2016. The first record of Amolops himalayanus (Anura: Ranidae) from Bhutan. Herpetol Bull, 136 (2016): 13–18
Orlov N. L., Ho T. C. 2007. Two new species of cascade ranids of Amolops genus (Amphibia: Anura: Ranidae) from Lai Chau Province (northwest Vietnam). Russ J Herpetol, 14: 211–228
Pham A. V., Sung N. B., Pham C. T., Le M. D., Ziegler T., Nguyen T. Q. 2019. A new species of Amolops (Anura: Ranidae) from Vietnam. Raffels Bul Zool, 67: 363–377
Pope C. H. 1929. Four new frogs from Fukien Province, China. Am Mus Nov, 352: 1–5
Pope C. H., Romer J. 1951. A New Ranid Frog (Staurois) from the Colony of Hongkong. Field Zool, 31: 609–612
Rao D. Q., Wilkinson J. A. 2007. A new species of Amolops (Anura: Ranidae) from southwest China. Copeia, 2007: 913–919
Ray P. 1992. Two new hill–stream frogs of the genus Amolops Cope (Amphibia: Anura: Ranidae) from Uttar Pradesh (India). Ind J Forest, 15: 345–350
Ray P. 1999. Systematic studies on the amphibian fauna of the district Dehradun, Uttar Pradesh, India. Zoological Survey of India, Dehradun, India
Ronquist F., Huelsenbeck J. P. 2003. MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19(12): 1572–1574
Sengupta S., Hussain B., Choudhury P., Gogoi J., Ahmed M., Choudhury N. 2008. A new species of Amolops (Anura: Ranidae) from Assam, north–eastern India. Hamadryad, 32: 5–12
Shah K. B., Tiwari S. 2004. Herpetofauna of Nepal: A Conservation Companion. Kathmandu, Nepal: IUCN Nepal
Smith M. A. 1923. On a collection of reptiles and batrachians from the island of Hainan. J Nat His Soc Siam, 6: 195–212
Smith M. A. 1940. The amphibians and reptiles obtained by Mr. Ronald Kaulback in Upper Burma. Rec Ind Mus, 42: 465–486
Stuart B. L., Bain R. H., Phimmachak S., Spence K. 2014. Phylogenetic Systematics of the Amolops monticola Group (Amphibia: Ranidae), with Description of a New Species from Northwestern Laos. Herpetologica, 66(1): 52–66
Su C., Yang D., Li S. 1986. A new species of Amolops from the Hengduan Shan Mountains. Acta Herpetol Sinica, 5: 204–206
Sun G. Z., Luo W. X., Sun H. Y., Zhang G. Y. 2013. A new species of Cascade Frog from Tibet. Forest Const, 5(1): 14–16
Sung Y., Hu P., Wang J., Liu H., Wang Y. Y. 2016. A new species of Amolops (Anura: Ranidae) from southern China. Zootaxa, 4170(3): 525–538
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res, 25(24): 4876–4882
Wu G. F., Tian W. S. 1995. A new Amolops species from southern Yunnan. Pages 51–52 in E. M. Zhao, editor. Amphibian Zoogeographic Division of China. A Symposium Issued to Celebrate the Second Asian Herpetological Meeting Held at Ashgabat, Turkmenistan 6 to 10 September 1995. Sichuan J Zool, Ashgabat, Turkmenistan
Yang D. T. 1991. Phylogenetic systematics of the Amolops group of ranid frogs of southeastern Asia and the Greater Sunda Islands. Fieldiana Zool, 63: 1–42
Yu G., Wu Z., Yang J. 2019. A new species of the Amolops monticola group (Anura: Ranidae) from southwestern Yunnan, China. Zootaxa, 4577(3): 548–560
Yuan Z., Jin J., Li J., Stuart B. L., Wu J. 2018. A new species of cascade frog (Amphibia: Ranidae) in the Amolops monticola group from China. Zootaxa, 4415(3): 498–512
Zhao, W. G., Rao D. Q., Lu S. Q., Dong B. J. 2005. Herpetological surveys of Xizang autonomous region 2. Medog. Sichuan J Zool, 24: 245–249


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