Weerachai SAIJUNTHA,Sutthira SEDLAK,Takeshi AGATSUMA,et al.Genetic Structure of the Red-spotted Tokay Gecko, Gekko gecko (Linnaeus, 1758) (Squamata: Gekkonidae) from Mainland Southeast Asia[J].Asian Herpetological Research(AHR),2019,10(2):69-78.[doi:10.16373/j.cnki.ahr.180066]
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Genetic Structure of the Red-spotted Tokay Gecko, Gekko gecko (Linnaeus, 1758) (Squamata: Gekkonidae) from Mainland Southeast Asia
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2019 VoI.10 No.2
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Genetic Structure of the Red-spotted Tokay Gecko, Gekko gecko (Linnaeus, 1758) (Squamata: Gekkonidae) from Mainland Southeast Asia
Weerachai SAIJUNTHA1 Sutthira SEDLAK1 Takeshi AGATSUMA2 Kamonwan JONGSOMCHAI3 Warayutt PILAP1 Watee KONGBUNTAD4 Wittaya TAWONG5 Warong SUKSAVATE1 Trevor N. PETNEY6 and Chairat TANTRAWATPAN7
1 Walai Rukhavej Botanical Research Institute, Biodiversity and Conservation Research Unit, Mahasarakham University, Maha Sarakham 44150, Thailand
2 Department of Environmental Medicine, Kochi Medical School, Kochi University, Oko, Nankoku, Kochi 783-8505, Japan
3 Department of Anatomy, School of Medical Science, Phayao University, Phayao 56000, Thailand
4 Program in Biotechnology, Faculty of Science, Maejo University, Chiang Mai 50290, Thailand
5 Department of Agricultural Science, Faculty of Agriculture, Natural Resources and Environment, Naresuan University, Phitsanulok 65000, Thailand
6 Department of Paleontology and Evolution, State Museum of Natural History Karlsruhe, Erbprinzenstrasse 13, Karlsruhe 76133, Germany
7 Division of Cell Biology, Department of Preclinical Sciences, Faculty of Medicine, Thammasat University, Rangsit Campus, Pathumthani 12120, Thailand
Genetic diversity Gene flow Natural barrier Phylogenetic tree Haplotype network
This study was performed to explore the genetic diversity and genetic structure of red-spotted tokay geckos (Gekko gecko) from 23 different geographical areas in Thailand, Lao PDR and Cambodia. The mitochondrial tRNA-Gln/tRNA-Met/partial NADH dehydrogenase subunit 2 from 166 specimens was amplified and sequenced. A total of 54 different haplotypes were found. Highly significant genetic differences occurred between populations from different localities. The haplotype network revealed six major haplogroups (G1 to G6) belonging to different clades (clade A–E). Clade D and clade E were newly observed in this study. Haplogroup G4 (clade D) was a sympatric population with haplogroup G1 (clade B). The populations from northern Thailand were divided into two distinct haplogroups separated by mountain range. Genetic structure and genetic differentiation of the tokay in Southeast Asia was related to the geographical region sampled, spatial distance and natural barriers. Our results indicate that red-spotted tokay geckos from mainland Southeast Asia are cryptically diverse. Morphological comparisons, in addition to an intensive genetic investigation covering the whole species range, are needed to clarify the systematic and population structure of this species group.


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Last Update: 2019-06-25