Meihua ZHANG,Liang FEI,Changyuan YE,et al.A New Species of Genus Microhyla (Amphibia: Anura: Microhylidae) from Zhejiang Province, China[J].Asian Herpetological Research(AHR),2018,9(3):135-148.[doi:10.16373/j.cnki.ahr.180032]
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A New Species of Genus Microhyla (Amphibia: Anura: Microhylidae) from Zhejiang Province, China
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2018 VoI.9 No.3
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A New Species of Genus Microhyla (Amphibia: Anura: Microhylidae) from Zhejiang Province, China
Meihua ZHANG12 Liang FEI1 Changyuan YE1 Yufan WANG3 Bin Wang1* and Jianping JIANG1*
1 CAS Key Laboratory of Mountain Ecological Restoration and Bioresource Utilization and Ecological Restoration and Biodiversity Conservation Key Laboratory of Sichuan Province, Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
3 Zhejiang Forest Resource Monitoring Center, Hangzhou 310020, China
Microhyla morphological comparison new species phylogenetic analysis taxonomy Zhejiang
We described a new species, Microhyla beilunensis sp. nov., from Zhejiang Province of China. Phylogenetic analyses based on the mitochondrial 12S, 16S and CO1 gene sequences suggested that the new taxon was distinctly separated from its congeners and closed to M. mixtura and M. okinavensis. Morphologically, the new species could be identified from its congeners except M. mixtura by several characters: (1) rudimentary webs on toe base; (2) absence of disks and dorsal median longitudinal grooves on finger tips; (3) presence of disks and dorsal median longitudinal grooves on toe tips. As well, the new species could be identified from topotype M. mixtura by the combination of characters: (1) apart from the stripes, bar-shaped and oval-shaped patterns, the rounded spots present on the dorsum of body and legs; (2) the outer metacarpal tubercles prominently larger than the inner one; (3) of males, the ratios of HW, IND, UEW and LAW to SVL of the new species were significantly larger than those of M. mixtura (P < 0.01), and the ratios of SL, IOD, LAHL, HLL, TL, TFL and FL to SVL of the new species were significantly less than those of M. mixtura (P < 0.05).


Andersson L. G. 1942. A small collection of frogs from Annam collected in the years 1938-1939 by Bertil Bj?rkegren. Ark Zool Stockholm, 34(6): 1?11
Bain R. H., Nguyen. T. Q. 2004. Three new species of narrow-mouthed frogs (genus Microhyla) from Indochina, with comments on Microhyla annamensis and Microhyla palmipes. Copeia, 2004: 507?524
Blyth E. 1856. Report for October meeting, 1855. J Asiatic Soc Bengal, 24: 711?723
Boulenger G. A. 1884. Descriptions of new species of reptiles and batrachians in the British Museum—Part. II. Ann Mag Nat Hist Ser 5, 13: 396?398
Boulenger G. A. 1897. Descriptions of new Malay frogs. Ann Mag Nat Hist Ser 6, 19: 106?108
Boulenger G. A. 1900. Descriptions of new batrachians and reptiles from the Larut Hills, Perak. Ann Mag Nat Hist Ser 7, 6:186?193
Chaves J. A., Weir J. T., Smith T. B. 2011. Diversification in Adelomyia hummingbirds follows Andean uplift. Mol Ecol, 20(21): 4564?4576
Che J., Chen H. M., Yang J. X., Jin J. Q., Jiang K. E., Yuan Z. Y., Murphy R. W., Zhang Y. P. 2012. Universal COI primers for DNA barcoding amphibians. Mol Ecol Resour, 12(2): 247?258
Chen B. H. 1991. The amphibian and reptilian fauna of Anhui. Hefei, China: Publishing House of Science and Technology (In Chinese)
Chen X. H., Tao L. K., Shi L., Meng X. P. 2003. Discovery of Microhyla mixtura in Henan Province. Chin J Zool, 38(2): 89?90
Craw D., Burridge C. P., Upton P., Rowe D. L., Waters J. M. 2008. Evolution of biological dispersal corridors through a tectonically active mountain range in New Zealand. J Biogeogr, 35: 1790?1802
Darriba D., Taboada G. L., Doallo R., Posada D. 2012. jModelTest 2: more models, new heuristics and parallel computing. Nat Methods, 9: 772?772
Das I., Yaakob N., Sukumaran J. 2007. A new species of Microhyla (Anura: Microhylidae) from the Malay Peninsula. Hamadryad, 31: 304?314
Duméril A. M. C., Bibron G. 1841. Erpétologie Genérale ou Histoire Naturelle Complète des Reptiles. Vol 8, Librarie Enclyclopedique de Roret, Paris
Dutta S. K., Ray P. 2000. Microhyla sholigari, a new species of microhylid frog (Anura: Microhylidae) from Karnataka, India. Hamadryad, 25: 38?44
Fei L., Ye C. Y. 1982. The distributional characteristics of Amphibia in Hubei Province, including description of a new species. Acta Zool Sinica, 28(3): 293?301 (In Chinese)
Fei L., Hu S. Q., Ye C. Y., Huang, Y. Z. 2009. Fauna Sinica. Amphibia, Anura. Beijing, China: Science Press (In Chinese)
Fei L., Ye C. Y., Jiang, J. P. 2012. Colored Atlas of Chinese Amphibians and Their Distributions. Chengdu, China: Sichuan Publishing House of Science and Technology (In Chinese)
Fei L., Ye C. Y., Jiang J. P., Xie F., Huang Y. Z. 2005. An Illustrated Key to Chinese Amphibians. Chengdu, China: Sichuan Publishing Group and Sichuan Publishing House of Science and Technology (In Chinese)
Fei L., Ye C. Y., Xie F., Cai C. M. 1999. Discovery of Microhyla mixtura (Amphibia: Anura: Microhylidae) in Zhejiang Province. Beijing, China: China Forestry Publishing House (In Chinese)
Fernando P., Siriwardhane M. 1996. Microhyla karunaratnei (Anura: Microhylidae), a new species of frog endemic to Sri Lanka. J S Asian Nat Hist, 2: 135?142
Frost D. R. 2018. Amphibian Species of the World: an Online Reference. Version 6.0. American Museum of Natural History, New York, USA. Accessible from: (accessed 11 April 2018)
Gosner K. L. 1960. A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16(3): 183-190
Hallowell E. 1861. Report upon the Reptilia of the North Pacific Exploring Expedition, under command of Capt. John Rogers, U.S. N 1860. Pro Acad Nat Sci Philadelphia, 12: 480?510
Hasan M. K., Islam M. M., Kuramoto M., Kurabayashi A., Sumida M. 2014. Description of two new species of Microhyla (Anura: Microhylidae) from Bangladesh. Zootaxa, 3755: 401?418
Howlader M. S. A., Nair A., Gopalan S.V., Meril? J. 2015. A new species of Microhyla (Anura: Microhylidae) from Nilphamari, Bangladesh. PloS ONE, 10: e0119825
Hu S. Q., Zhao E. M., Liu C. C. 1966. A herpetological survey of the Tsinling and Ta-Pa Shan region. Acta Zool Sinica, 1966, 18: 57?89 (in Chinese)
Huang M. H., Jin Y. L., Cai C. M. 1990. Fauna of Zhejiang: Amphibia/Reptilia. Hangzhou, China: Zhejiang Science and Technology Publishing House (in Chinese)
Hurvich C. M., Tsai C. L. 1989, Regression and time series model selection in small samples. Biometrika, 76(2): 297?307
Huson D. H., Bryant D. 2006. Application of phylogenetic networks in evolutionary studies. Mol Biol and Evol, 23: 254?267
Inger R. F. 1989. Four new species of frogs from Borneo. Malayan Nat J, 42: 229?243
Inger R. F., Frogner K. J. 1979. New species of narrow-mouth frogs (genus Microhyla) from Borneo. Sarawak Mus J, 27: 311?322
Jerdon T. C. 1853. Catalogue of reptiles inhabiting the peninsula of India. J Asiatic Soc Bengal, 22: 462?479
Khatiwada J. R., Shu G. C., Wang S. H., Thapa A., Wang B., Jiang J. P. 2017. A new species of the genus Microhyla (Anura: Microhylidae) from Eastern Nepal. Zootaxa, 4254: 221?239
Kocher T. D., Thomas W. K., Meyer A., Edwards S.V., P??bo S., Villablanca F. X., Wilson A. C. 1989. Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. P Natl Acad Sci, 86: 6196?6200
Matsui M. 2011. Taxonomic revision of one of the Old World’s smallest frogs, with description of a new Bornean Microhyla (Amphibia, Microhylidae). Zootaxa, 2814: 33?49
Matsui M., Hamidy A., Belabut D. M., Ahmad N., Panha S., Sudin A., Khonsue W., Oh H. S., Yong H. S., Jiang, J. P. 2011. Systematic relationships of Oriental tiny frogs of the family Microhylidae (Amphibia, Anura) as revealed by mtDNA genealogy. Mol Phylogenet Evol, 61: 167?176
Matsui M., Hamidy A., Eto K. 2013. Description of a new species of Microhyla from Bali, Indonesia (Amphibia, Anura). Zootaxa, 3670: 579?590
Parker H. W. 1928. The brevicipitid frogs of the genus Microhyla. Ann Mag Nat Hist Ser 10, 2: 473?499
Parker H. W. 1934. A monograph of the frogs of the family Microhylidae. London, England: British Museum (Natural History)
Parker H., Osman-Hill W. 1948. Frogs of the genus Microhyla from Ceylon. J Nat Hist, 1: 759?764
Pillai R. S. 1977. On two frogs of the family Microhylidae from Andamans including a new species. Proc Indian Acad Sci, Sect B, 86:135?138
Poyarkov Jr. N. A., Vassilieva A. B., Orlov N. L., Galoyan E. A. Tran T. A. D., Duong T. T. L., Kretova V. D., Geissler P. 2014. Taxonomy and distribution of narrow-mouth frogs of the genus Microhyla Tschudi, 1838 (Anura: Microhylidae) from Vietnam with descriptions of five new species. Russ J Herpetol, 21: 89?148
Ronquist F., Teslenko M., van der Mark P., Ayres D. L., Darling A., H?hna S., Larget B., Liu L., Suchard M. A., Huelsenbeck J. P. 2012. MrBayes 3.2: efficient Bayesian phylogenetic inference and model choice across a large model space. Syst Biol, 61: 539?542
Schenkel E. 1901. Achter Nachtrag zum Katalog der herpetologischen Sammlung des Basler Museums. Verhandl Naturforsch Ges Basel, 13: 142?199
Seshadri K. S., Singal R., Priti H., Ravikanth G., Vidisha M. K., Saurabh S., Pratik M., Gururaja K. V. 2016. Microhyla laterite sp. nov., a new species of Microhyla Tschudi, 1838 (Amphibia: Anura: Microhylidae) from a laterite rock formation in South West India. PloS ONE, 11: e0149727
Simon C., Frati F., Beckenbach A., Crespi B., Liu H., Flook P. 1994. Evolution, weighting, and phylogenetic utility of mitochondrial gene sequences and a compilation of conserved polymerase chain reaction primers. Ann entomol Soc Am, 87: 651?701
Smith M. A. 1923. Notes on reptiles and batrachians from Siam and Indo-China (No. 2). J Nat Hist Soc Siam, 6: 47?53
Stamatakis A. 2006. RA×ML-VI-HPC: maximum likelihood-based phylogenetic analysis with thoughts of taxa and mixed models. Bioinformatics, 22(21): 2688
Stejneger L. 1901. Diagnoses of eight new batrachians and reptiles from the Riu Kiu Archipelago, Japan. Proc Biol Soc Wash, 14: 189?191
Tamura K., Stecher G., Peterson D., Filipski A., Kumar S. 2013. MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol, 30(12): 2725?2729
Tschudi J. J. v. 1838. Classification der Batrachier mit Berücksichtigung der fossilen Thiere dieser Abtheilung der Reptilien. Petitpierre, Neuch?el.
Vences M., Thomas M., van der Meijden A., Chiari Y., Vieites D. R. 2005. Comparative performance of the 16S rRNA gene in DNA barcoding of amphibians. Front Zool, 2 (1): 5
Vogt T. 1911. Beitrag zur Amphibien-fauna der Insel Formosa. Sitzungsb Ges Naturforsch Fr Berlin, 1911: 179?184
Watters J. L., Cummings S. T., Flanagan R. L., Siler C. D. 2016. Review of morphometric measurements used in anuran species descriptions and recommendations for a standardized approach. Zootaxa, 4072(4): 477?495
Wijayathilaka N., Garg S., Senevirathne G., Karunarathna N., Biju S. D., Meegaskumbura M. 2016. A new species of Microhyla (Anura: Microhylidae) from Sri Lanka: an integrative taxonomic approach. Zootaxa, 4066: 331?342


Last Update: 2018-09-26