Xiuli YU,Yu DU,Mengchao FANG,et al.The Mitochondrial Genome of Pseudocalotes microlepis (Squamata: Agamidae) and its Phylogenetic Position in Agamids[J].Asian Herpetological Research(AHR),2018,9(1):24-34.[doi:10.16373/j.cnki.ahr.170035]
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The Mitochondrial Genome of Pseudocalotes microlepis (Squamata: Agamidae) and its Phylogenetic Position in Agamids
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2018 VoI.9 No.1
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The Mitochondrial Genome of Pseudocalotes microlepis (Squamata: Agamidae) and its Phylogenetic Position in Agamids
Xiuli YU1 Yu DU23 Mengchao FANG1 Hong LI2 and Longhui LIN1*
1 Hangzhou Key Laboratory for Ecosystem Protection and Restoration, College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China
2 Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, China
3 Hainan Key Laboratory of Herpetology, College of Tropical Biology and Agronomy, Hainan Tropical Ocean University, Sanya 572022, China
mitogenome Pseudocalotes microlepis agamids phylogeny
We describe the complete mitochondrial genome of a small-scaled forest agamid (Pseudocalotes microlepis), which is 17 873 bp in size, containing 13 PCGs, 2 rRNAs, 22 tRNAs and non-coding regions. The mitogenome has a typical gene order among squamates. 13 PCGs include 2 start codons (ATG and ATA), 3 stop codons (TAG, TAA and AGG), and an incomplete stop codon (T-). Codon usage analyses showed that CUA-Leu1 and CGG-Arg are the most frequently and rarely used codon, respectively. All 22 tRNAs were predicted to form canonical cloverleaf secondary structures, except for two tRNAs (tRNACys and tRNASer (AGY)) lacking the dihydorouridine (DHU) arm. The large non-coding region (control region) is 2 687 bp long (28.3% T, 18.2% C, 42.3% A, and 11.2% G), with four different types of repeating sequences. The phylogenetic tree resulting from BEAST analyses based on concatenated 2 rRNAs and 13 PCGs in sequence revealed that the newly sequenced P. microlepis, where the genus Acanthosaura were aggregated. Together with Calotes versicolor, they constitute the subfamily Draconinae.


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Last Update: 2018-03-27