Xiaowei SONG,Jinghan SONG,Honghong SONG,et al.A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces[J].Asian Herpetological Research(AHR),2018,9(1):1-12.[doi:10.16373/j.cnki.ahr.170062]
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A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2018 VoI.9 No.1
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A Robust Noninvasive Approach to Study Gut Microbiota Structure of Amphibian Tadpoles by Feces
Xiaowei SONG12* Jinghan SONG1 Honghong SONG1 Qi ZENG1 and Keke SHI1
1 College of Life Sciences, Xinyang Normal University, Xinyang 464000, China
2 Institute for Conservation and Utilization of Agro-bioresources in Dabie Mountains, Xinyang Normal University, Xinyang 464000, China
Bufo gargarizans DNA extraction intestinal microflora Phenol-chloroform 16S rDNA
The 16S rDNA amplicon high-throughput sequencing technique provides a robust and inexpensive approach to detect the gut microbiota of amphibians. Since different experimental protocols generate technical biases in drawing the gut microbiota profiles, the integrative analysis of gut microbiota produced by different studies must be performed with circumspection. In this study, we compared the efficacy of two DNA extraction methods (i.e., a phenol-chloroform method and TIANamp Stool DNA Kit) in describing intestinal and fecal bacterial communities of transplanted Asiatic toad (Bufo gargarizans) tadpoles. In terms of the DNA extraction quality (i.e., DNA purity and yield rate) and the consistency in between fecal and intestinal microbiota structures (i.e., α and β diversity indices), the phenol-chloroform method was more robust than this commercial stool kit in profiling gut microbiota of tadpoles with feces.


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Last Update: 2018-03-27