Zhiheng DU,Chunzhu XU and Fangyong NING.Isolation and Characterization of 15 Microsatellite DNA Loci for the Alpine Stream Frog Scutiger boulengeri (Anura: Megophryidae)[J].Asian Herpetological Research(AHR),2016,7(4):298-300.[doi:10.16373/j.cnki.ahr.150073]
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Isolation and Characterization of 15 Microsatellite DNA Loci for the Alpine Stream Frog Scutiger boulengeri (Anura: Megophryidae)
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2016 VoI.7 No.4
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Isolation and Characterization of 15 Microsatellite DNA Loci for the Alpine Stream Frog Scutiger boulengeri (Anura: Megophryidae)
Zhiheng DU Chunzhu XU and Fangyong NING*
Northeast Agricultural University, Harbin 150030, Heilongjiang, China
Alpine stream frogs Microsatellite DNA Tibetan Plateau Scutiger boulengeri
Fifteen microsatellite DNA markers were developed from a transcriptome assembly of the alpine stream frog (Scutiger boulengeri).To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven loci were variable, with the number of alleles ranging from one to six within each population. The expected and observed heterozygosities ranged from 0 to 0.78 and from 0 to 0.64, respectively. None of the loci was in linkage disequilibrium and one locus for one population was deviated from the Hardy-Weinberg equilibrium. We hope that these markers will offer useful tools in detecting population structure of S. boulengeri and in monitoring the fragile ecosystem of the Tibetan Plateau, where the species resides.


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Last Update: 2016-12-25