Baorong GENG,Lingling ZHANG,Yun JIANG,et al.Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay[J].Asian Herpetological Research(AHR),2016,7(4):251-257.[doi:10.16373/j.cnki.ahr.150058]

Click Copy

Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay

Share To：

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:

2016 VoI.7 No.4

Page:

251-257

Research Field:

Publishing date:

2016-12-25

Info

Title:

Genotoxicity of Three Avermectins on Polypedates megacephalus Tadpoles Using the Comet Assay

Author(s):

Baorong GENG^*;  Lingling ZHANG;  Yun JIANG;  Xiuping HUANG and Jinmei DAI

College of Life Sciences, Fujian Normal University, Fuzhou 350117, Fujian, China

Keywords:

Polypedates megacephalus;  tadpole;  avermectins;  abamectin;  ivermectin;  emamectin benzoate;  DNA damage;  comet assay

PACS:

-

DOI:

10.16373/j.cnki.ahr.150058

Abstract:

Avermectins are a new class of macrocyclic lactones derived from mycelia of the soil actinomycete, and are used as effective agricultural pesticides and antiparasitic agents. However, run-off from crops treated with avermectins may contaminate various bodies of water, and accumulated to certain concentrations to impact the development of aquatic animals. Here, we tested the genotoxicity of three avermectins (abamectin, ABM; ivermectin, IVM; and emamectin benzoate, EMB) on Polypedates megacephalus tadpoles by the alkaline single-cell gel electrophoresis assay. Tadpoles were treated for 48 h in the laboratory with different concentrations of these three agents, 0.006, 0.012, 0.018, 0.024, 0.030 mg/L for ABM, 0.003, 0.006, 0.009, 0.012, 0.015 mg/L for IVM and 0.04, 0.06, 0.08, 0.10, 0.12 mg/L for EMB, and then measured their DNA damage by the Comet assay tail factor %. The concentrations of resulted in highly significant increases in DNA damage of the tadpoles were found above the concentration threshold of 0.012 mg/L ABM, 0.003 mg/L IVM and 0.06 mg/L EMB and linear correlations between the intensity of DNA damage and the concentrations of these three avermectins. Our results showed clearly that avermectins caused dose dependent DNA damage on amphibian tadpoles, and there might be a control on the misuse of avermectins.

References:

Ankley G. T., Tietge J. E., Defoe D. L., Jensen K. M., Holcombe G. W., Durhan E. J., Diamond S. A. 1998. Effects of ultraviolet light and methoprene on survival and development of Rana pipiens. Environ Toxicol Chem, 17: 2530–2542
Bansod Y. V., Kharkar S. V., Raut A., Choudalwar P. 2013. Abamectin: an uncommon but potentially fatal cause of pesticide poisoning. Int J Res Med Sci, 1: 285–286
Berrill M., Bertram S., Pauli B. 1997. Effects of pesticides on amphibian embryos and tadpoles. In: Green D. M. (ed). Amphibians in decline: Canadian studies of a global problem. Society for the Study of Amphibians and Reptiles, St. Louis, MO. 233–245
Bing X., Ru S. G., Zhou W. L., Jia Y. G. 2008. Avermectin’s safety evaluation of environmental estrogenic activity and reproductive toxicity. J Wuhan Univ, 54: 745–750 (In Chinese)
Bloom R. A., Matheson J. C. 1993. Environmental assessment of avermectins by the US Food and Drug Administration. Vet Parasitol, 48: 281–294
Cai M. Z. 1979. Observations on reproductive habits of thirty-two anuran species of Fujian Province. J Fujian Nor Univ, (1): 71–79 (In Chinese)
Chen Z. X., Fang X. Q., Lin L., Geng B.R. 2011. Acute toxicity of emamectin benzoate on Rana zhenhaiensis tadpoles. J Ningde Teach Coll, 23: 21–23 (In Chinese)
Clements C., Ralph S., Petras M. 1997. Genotoxicity of select herbicides in Rana catesbeiana tadpoles using the alkaline sing-cell gel DNA electrophoresis (Comet) assay. Environ Mol Mutagen, 29: 277?288
Collins A. R., Ma A. G., Duthie S. J. 1995. The kinetics of repair of oxidative DNA damage (strand breaks and oxidised pyrimidines) in human cells. Mutation Res, 336: 69–77
Currie B. J., McCarthy J. S. 2010. Permethrin and Ivermectin for Scabies. N Engl J Med, 362: 717–725
Danishefsky S. J, Armistead D. M., Wincott F. E., Selnick H. G., Hungate R. 1989. The total synthesis of avermectin-A1A. J Am Chem Soc, 111: 2967–2980
Davidson C. 2004. Declining downwind: Amphibian population declines in California and historical pesticide use. Ecol Appl, 14: 1892?1902
Davies I. M., Gillibrand P. A., McHenery J. G., Rae G. H. 1998. Environment risk of ivermectin to sediment dwelling organisms. Aquaculture, 163: 29–46
Duellman W. E., Trueb L. 1994. Biology of amphibians. Baltimore: The John Hopkings University Press
Egeler P., Gilberg D., Fink G., Duis K. 2010. Chronic toxicity of ivermectin to the benthic invertebrates Chironomus riparius and Lumbriculus variegates. J Soils Sedime, 10: 368–376
Egerton J. R., Ostlind D. A., Blair L. S., Eary C. H., Suhayda D., Cifelli S., Riek R. F., Campbell W. C. 1979. Avermectins, new family of potent anthelmintic agents: efficacy of the B1a component. Antimicrob Agents Chemother. 15: 372–378
Elbetieha A., Da’as S. I. 2003. Assessment of antifertility activities of abamectin pesticide in male rats. Ecotoxicol Environ Saf, 55: 307–313
Fang X. Q., Chen Z. X., Lin L., Geng B. R. 2010. Genotoxicity of emamectin benzoate on Rana zhenhaiensis tadpoles. J Ningde Teach Coll, 22: 373–376 (In Chinese)
Fanigliulo A., Sacchetti M. 2008. Emamectin benzoate: new insecticide against helicoverpa armigera. Commun Agric Appl Biol Sci, 73: 651–653
Geng B. R., Lin L., Zhang Q. J., Zhong B.J. 2010. Genotoxicity of the pesticide dichlorvos and herbicide butachlor on Rana zhenhaiensis tadpoles. Asian Herpetol Res, 1: 118–122
Gosner K. L. 1960. A simplified table for staging anuran embryos and larvae with notes on identification. Herpetologica, 16: 183–190
Herd R. 1995. Endectocidal drugs: ecological risks and countermeasures. Int J Parasitol, 25: 875–885
Jencic V., Cerne M., Erzen N. K., Kobal S., Cerkvenik-Flajs V. 2006. Abamectin effects on rainbow trout (Oncorhynchus mykiss). Ecotoxicol, 15: 249–257
Jensen J., Krogh P. H., Sverdrup L. E. 2003. Effects of the antibacterial agents tiamulin, olanquindox and metronidazole and the anthelmintic ivermectin on the soil invertebrate species Folsomia fimetaria (Collembola) and Enchytraeus crypticus (Enchytraeidae). Chemosphere, 50:437–443
Jiang M., Peng Z. X., Wu H., Hu K., Huang X. X. 2008. Application of ivermectin in aquaculture and corresponding aqua-ecosystem risk. Fish Modern, 35: 47–50 (In Chinese)
Katharios P., Iliopoulou-Georgudaki J., Kapata-Zoumbos K., Spiropoulos S. 2002. Toxicity of intraperitoneally injected ivermectin in sea bream, Sparus aurata. Fish Physiol Biochem, 25: 99–108
Krieger R. I. 2001. Handbook of pesticide toxicology second edition volume 1. American: Academic Press
Madsen M., Overgaard-Nielsen B., Holter P., Pedersen O. C., Br?chner-Jespersen J., Vagn-Jensen K. M., Nansen P., Gr?nvold J. 1990. Treating cattle with ivermectin: effects on the fauna and decomposition of dung pats. J Appl Ecol, 27: 1–15
Mann R. M., Bidwell J. R. 2001. The acute toxicity of agricultural surfactants to the tadpoles of four Australian and two exotic frogs. Environ Poll, 1l4: 195–205
Martini F., Tarazona J. V., Pablos M. V. 2012. Are fish and standardized FETAX assays protective enough for amphibians? A case study on Xenopus laevis larvae assay with biologically active substances present in livestock wastes. Sci World J, 2012: 1–6
Prichard R., Ménez C., Lespine A. 2012. Moxidectin and the avermectins: Consanguinity but not identity. Int J Parasitol: Drugs and Drug Resistance, 2: 134–153
Putter J. G., Mac Connell F. A., Preiser F. A., Haidri A. A., Rishich S. S., Dybas R. A. 1981. Avermectins: novel class of insecticides, acaricides and nematicides from a soil microorganism. Experientia, 37: 963–964
Ralph S., Petras M., Pandrangi R., Vrzoc M. 1996. Alkaline single cell gel (comet) assay and genotoxicity monitoring using two species of tadpoles. Environ Mol Mut, 28: 112–120
Reddy P. P. 2013. Recent advances in crop protection. Springer: 13–24
R?mbke J., Floate K. D., Jochmann R., Sch?fer M. A., Puniamoorthy N., Kn?be S., Lehmhus J., Rosenkranz B., Scheffczyk A., Schmidt T., Sharples A., Blanckenhorn W. U. 2009. Lethal and sublethal toxic effects of a test chemical (ivermectin) on the yellow dung fly Scathophaga stercoraria based on a standardized international ring test. Environ Toxicol Chem, 28: 2117–2124
R?mbke J., Krogh K. A., Moser T., Scheffczyk A., Liebig M. 2010. Effects of the veterinary pharmaceutical ivermectin on soil invertebrates in laboratory tests. Arch Environ Contam Toxicol, 58: 332–340
Sanderson H., Laird B., Pope L., Brain R., Wilson C., Johnson D., Bryning G., Peregrine A. S., Boxall A., Solomon K. 2007. Assessment of the environmental fate and effects of ivermectin in aquatic mesocosms. Aquat Toxicol, 85: 229–240
Singh G., Chahil G. S., Jyot G., Battu R. S., Singh B. 2013. Degradation dynamics of emamectin benzoate on cabbage under subtropical conditions of Punjab, India. Bull Environ Contam Toxicol, 91:129–133
Stuart S. N., Chanson J. S., Cox N. A., Young B. E., Rodrigues A. S., Fischman D. L., Waller R. W. 2004. Status and trends of amphibian declines and extinctions worldwide. Science, 306: 1783–1786
Sun J., Meng S. Q. 2009. Status and development trend of avermectin in Chinese market. World Pestic, 31(Suppl. 2): 18–21
Tang W. W., Lu Y. X., Mu B., Yin X. F., Zhang L. L. 2011. Research progress in emamectin benzoate toxicology. Chin J Foren Med, 26: 210–212
Tice R.R. 1995. Applications of the single cell gel assay to environmental biomonitoring for genotoxic pollutants. In: Butterworth B. M., Corkum L. D., Guzma?n-Rinco?n J. ed. Biomonitoring and Bio-markers as Indicators of Environmental Change. New York: Plenum Press, 69–79
Tice R. R., Agurell E., Anderson D., Burlinson B., Hartmann A., Kobayashi H., Miyamae Y., Rojas E., Ryu J. C., Sasaki Y. F. 2000. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mut, 35: 206–221
Tisler T., Erzen N. K. 2006. Abamectin in the aquatic environment. Ecotoxicol, 15: 495–502
Tyler M. J. 1994. Australian frogs: A natural history. Chatswood: Reed Books
Valic E., Jahn O., P?pke O., Winker R., Wolf C., Rüdiger.W. H. 2004. Transient increase in micronucleus frequency and DNA effects in the comet assay in two patients after intoxication with 2,3,7,8-tetrachlorodibenzo-p-dioxin. Int Arch Occup Environ Heal, 77: 301–306
Wang D. D., Zhao E. M. 2013. Study on toxicological effect of avermectins on Pelophylax nigromaculatus. Sichuan J Zoo, 32: 334–342 (In Chinese)
Wang M. Z., Jia X. Y. 2009. Low levels of lead exposure induce oxidative damage and DNA damage in the testes of the frog Rana nigromaculata. Ecotoxicol, 18: 94–99
Wei F. L., Zhu J. W., Li S. N., Zhu G. N. 2008. Acute toxicity of emamectin benzoate on environmental organism. Pestic Sci Admin, 29: 19–24 (In Chinese)
Xie F., Lau M. W. N., Stuart S. N., Chanson J. S., Cox N. A., Fischman D. L. 2007. Conservation needs of amphibians in China: A review. Sci Chin Series C: Life Sciences, 50(2): 265–276
Xu H. R., Yang R. B., Fu Q., Liao H. Y. 2010. Abamectin residue in water, soil and rice. Environ Sci Manage, 35: 35–37 (In Chinese)
Yu X. L., Cheng C. H., Zhang Q. 2007. The primary study about immune effects of 1.8 % AVM latex on mice. Acta Acad Med Zunyi, 30: 254–256 (In Chinese)
Zhang L. L., Li Q. Y., Geng B. R. 2014. Genotoxicity of ivermectin on Polypedates megacephalus tadpoles. J Fujian Nor Univ, 30: 106–110 (In Chinese)

Memo

Memo:

-

Common functions