Tianlong FU,Shan SUN,Chaonan ZHU and Yanfu QU.Isolation and Characterization of Nine Microsatellite Markers for Red-backed Ratsnake, Elaphe rufodorsata[J].Asian Herpetological Research(AHR),2016,7(3):227-228.[doi:10.16373/j.cnki.ahr.150065]
Click Copy

Isolation and Characterization of Nine Microsatellite Markers for Red-backed Ratsnake, Elaphe rufodorsata
Share To：

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2016 VoI.7 No.3
Page:
227-228
Research Field:
Publishing date:
2016-09-25

Info

Title:
Isolation and Characterization of Nine Microsatellite Markers for Red-backed Ratsnake, Elaphe rufodorsata
Author(s):
Tianlong FU;  Shan SUN;  Chaonan ZHU and Yanfu QU*
Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210023, Jiangsu, China
Keywords:
Colubridae;  Elaphe rufodorsata;  Microsatellite markers;  Polymorphism;  PCR primers
PACS:
-
DOI:
10.16373/j.cnki.ahr.150065
Abstract:
The red-backed ratsnake (Elaphe rufodorsata) is widely distributed in East Asia, especially China. This species is a common snake in plain river network region. In the past several decades, E. rufodorsata has dramatically declined due to the effect of human activities and over hunting for traditional Chinese medicine. We developed nine species-speci?c microsatellite loci in 190 individuals collected from Huzhou, Zhejiang province in China. These markers revealed a high degree of genetic diversity (13–41 alleles per locus) and heterozygosity (HO ranged from 0.266 to 0.941, and HE ranged from 0.851 to 0.937). No locus exhibited signi?cant deviations from Hardy–Weinberg equilibrium. There was no evidence of linkage disequilibrium among pairs of loci. These microsatellite markers were described in our study will be valuable tools for the long term management and population-level studies (e.g. the population structure, genetic diversity and variation, individual paternity and evolutionary history) of the species.

References:

Hua L., Mao L. X., Chen C., Gao J. F., Lin L. H. 2012. Isolation and characterization of microsatellite loci in the Reevese’s butterfly lizard Leiolepis reevesii (Agamidae). Conserv Genet Resour, 4: 791–794
Rice W. R. 1989. Analyzing tables of statistical tests. Evolution, 43: 223–225
Wang R. F., Wu Y. Q., Liu Z., Qu Y. F. 2014. Isolation and characterization of microsatellite loci in the Ussuri mamushi (Gloydius ussuriensis). Conserv Genet Resour, 6: 783–784
Wu Y. Q., Wang R. F., Lin L. H., Qu Y. F. 2014. Microsatellite primer development for the king ratsnakes Elaphe carinata (Colubridae). Conserv Genet Resour, 6: 211–212
Zhao E. M. 1998. Crotalinae. In Zhao EM, Huang MH, Zong Y (Eds.), Fauna Sinica, Reptilia, Vol. 3 (Squamata: Serpentes). Beijing: Science Press

Memo

Memo:
-
Last Update: 2016-09-25