Danna YU,Rongquan ZHENG,Qinfang LU,et al.Genetic Diversity and Population Structure for the Conservation of Giant Spiny Frog (Quasipaa spinosa) Using Microsatellite Loci and Mitochondrial DNA[J].Asian Herpetological Research(AHR),2016,7(2):75-86.[doi:10.16373/j.cnki.ahr.150040]
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Genetic Diversity and Population Structure for the Conservation of Giant Spiny Frog (Quasipaa spinosa) Using Microsatellite Loci and Mitochondrial DNA
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2016 VoI.7 No.2
Page:
75-86
Research Field:
Publishing date:
2016-06-25

Info

Title:
Genetic Diversity and Population Structure for the Conservation of Giant Spiny Frog (Quasipaa spinosa) Using Microsatellite Loci and Mitochondrial DNA
Author(s):
Danna YU1;  Rongquan ZHENG1; 2*;  Qinfang LU1;  Guang YANG2; 3;  Yao FU1 and Yun ZHANG1
1 Institute of Ecology, Zhejiang Normal University, Jinhua 321004, Zhejiang, China
2 Key Lab of Wildlife Biotechnology and Conservation and Utilization of Zhejiang Province, Jinhua, Zhejiang 321004, China
3 Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life Sciences, Nanjing Normal University, Nanjing 210046, Jiangsu, China
Keywords:
sexual selection;  acoustic communication;  male-male competition;  phonotaxis tests;  coevolution
PACS:
-
DOI:
10.16373/j.cnki.ahr.150040
Abstract:
The giant spiny frog (Quasipaa spinosa) is an endangered species with a relatively small distribution limited to southern China and Northern Vietnam. This species is becoming increasingly threatened because of over-exploitation and habitat degradation. This study provides data on the genetic diversity and population genetic structure of the giant spiny frog to facilitate the further development of effective conservation recommendations for this economically important but threatened species. We examined 10 species-specific microsatellite loci and Cyt b genes (562 bp) collected from 13 wild populations across the entire range of this species. Results of 10 microsatellite loci analysis showed a generally high level of genetic diversity. Moreover, the genetic differentiation among all 12 populations was moderate to large (overall FST = 0.1057). A total of 51 haplotypes were identified for Cyt b, which suggests high haplotype nucleotide diversities. Phylogeographic and population structure analyses using both DNA markers suggested that the wild giant spiny frog can be divided into four distinct major clades, i.e., Northern Vietnam, Western China, Central China, and Eastern China. The clades with significant genetic divergence are reproductively isolated, as evidenced by a high number of private alleles and strong incidence of failed amplification in microsatellite loci. Our research, coupled with other studies, suggests that Q. spinosa might be a species complex within which no detectable morphological variation has been revealed. The four phylogenetic clades and some subclades with distinct geographical distribution should be regarded as independent management units for conservation purposes.

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Last Update: 2016-06-25