Liang QIAO,Weizhao YANG,Hu NIE and Jiapan HU.Isolation and Characterization of 17 Microsatellite DNA Loci for Odorrana margaretae (Anura: Ranidae)[J].Asian Herpetological Research(AHR),2015,6(2):156-158.[doi:10.16373/j.cnki.ahr.140076]
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Isolation and Characterization of 17 Microsatellite DNA Loci for Odorrana margaretae (Anura: Ranidae)
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2015 VoI.6 No.2
Page:
156-158
Research Field:
Publishing date:
2015-06-25

Info

Title:
Isolation and Characterization of 17 Microsatellite DNA Loci for Odorrana margaretae (Anura: Ranidae)
Author(s):
Liang QIAO1; 2*;  Weizhao YANG1;  Hu NIE3 and Jiapan HU3
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China
2 College of Life Science and Technology, Xinxiang Medical University, Xinxiang 453003, Henan, China
3 College of Life Sciences, Sichuan University, Chengdu 610041, Sichuan, China
Keywords:
Microsatellite DNA loci;  Transcriptome;  Odorrana margaretae;  Cross?species amplification test
PACS:
-
DOI:
10.16373/j.cnki.ahr.140076
Abstract:
We isolated and characterized 17 microsatellite DNA loci for the odorous frog Odorrana margaretae from its transcriptome sequence data. These loci were screened with 24 individuals from Mt. Emei. All loci were polymorphic, with the number of alleles ranging from 2 to 8. The observed and expected heterozygosity, polymorphism information content, ranged from 0.04 to 1, 0.04 to 0.81, and 0.040 to 0.763, respectively. All loci were in linkage equilibrium and six loci were significantly deviated from Hardy?Weinberg equilibrium after sequential Bonferroni corrections. Cross?species amplification test was conducted for ten odorous frog species, and 12 loci were amplifiable in most species. With the high cross?species amplification rates, these markers will provide useful molecular tools for conservation genetic and phylogeographic studies on the genus Odorrana and Bamburana.

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Last Update: 2016-01-25