Huayuan HUANG,Dan LUO,Cong Guo,et al.Genetic Analysis of Multiple Paternity in an Endangered Ovoviviparous Lizard Shinisaurus crocodilurus[J].Asian Herpetological Research(AHR),2015,6(2):150-155.[doi:10.16373/j.cnki.ahr.140090]

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Genetic Analysis of Multiple Paternity in an Endangered Ovoviviparous Lizard Shinisaurus crocodilurus

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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:

2015 VoI.6 No.2

Page:

150-155

Research Field:

Publishing date:

2015-06-25

Info

Title:

Genetic Analysis of Multiple Paternity in an Endangered Ovoviviparous Lizard Shinisaurus crocodilurus

Author(s):

Huayuan HUANG¹; 2;  Dan LUO³; 4;  Cong Guo¹;  Zhuo TANG²;  Zhengjun WU^3* and Jinping CHEN^4*

¹ College of Life Science, Sichuan University, Chengdu 610065, Sichuan, China
² Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
³ Key Laboratory of Rare and Endangered Animal Ecology, College of Life Science, Guangxi Normal University, Guilin 541004, Guangxi, China
⁴ Guangdong Key Laboratory of Integrated Pest Management in Agriculture, Guangdong public Laboratory of Wild Animal Conservation and Utilization, Guangdong Entomological Institute, Guangzhou 510260, China

Keywords:

Shinisaurus crocodilurus;  Mating system;  Paternity assessment;  Saliva sample;  Microsatellite;  Polygyny;  Polyandry

PACS:

-

DOI:

10.16373/j.cnki.ahr.140090

Abstract:

The crocodile lizard (Shinisaurus crocodilurus) is an ovoviviparous lizard belonging to a monotypic family that originated during the end of the quaternary ice age. A rare species in the wild, the crocodile lizard was listed in CITES Appendix?II . Knowledge of the reproductive biology and mating system of this species is important for designing conservation strategies and improving genetic variation. To investigate the paternity of the crocodile lizards and to interpret their reproductive behaviour, we collected saliva from females, potential fathers and offspring in a semi-natural enclosure experiment and analyzed the paternity of the crocodile lizard using 12 microsatellite genetic loci. The overall observed incidence of multiple paternity was 42.9% (6 of 14 clutches) and Fis was 0.089?±?0.056. These results indicate that the primary mating mode of the crocodile lizard is that males are polygynous while with females are polyandrous, and there is multiple paternity among offspring of the same mother.

References:

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