Weizhao YANG,Jie WANG,Jianping JIANG,et al.Development and Characterization of Fifteen Polymorphic Microsatellites for Extremely Endangered Salamander, Echinotriton chinhaiensis[J].Asian Herpetological Research(AHR),2014,5(4):281-293.[doi:10.3724/SP.J.1245.2014.00281]

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Development and Characterization of Fifteen Polymorphic Microsatellites for Extremely Endangered Salamander, Echinotriton chinhaiensis

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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:

2014 VoI.5 No.4

Page:

281-293

Research Field:

Short Notes

Publishing date:

2014-12-25

Info

Title:

Development and Characterization of Fifteen Polymorphic Microsatellites for Extremely Endangered Salamander, Echinotriton chinhaiensis

Author(s):

Weizhao YANG;  Jie WANG;  Jianping JIANG;  Cheng LI and Feng XIE^*

Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China

Keywords:

Echinotriton chinhaiensis;  polymorphic microsatellite loci;  population genetics;  conservation biology

PACS:

-

DOI:

10.3724/SP.J.1245.2014.00281

Abstract:

Echinotriton chinhaiensis is a critically endangered salamander and its distribution is restricted to Ningbo, Zhejiang Province of China. In this study, we developed and characterized fifteen polymorphic microsatellite loci for E. chinhaiensis from dinucleotide- and tetranucleotide- enriched library. The number of alleles ranges from 4 to 12 with an average of 7.27 alleles per locus. The observed and expected heterozygosities values were from 0.250 to 0.844 and 0.511 to 0.872 with an average value of 0.596 and 0.722; respectively. The polymorphic microsatellite loci described in this paper are useful in the further study on genetic diversity and gene flow, which would be helpful to formulate effective conservation strategies for the E. chinhaiensis.

References:

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Liu C., Xie F., Jiang J. P., Zheng Z. H., Liu X. T., Liu Y., Wu M. F. 2010. Annual reproduction comparision and analysis of Chinhai Salamander (Echinotriton chinhaiensis) Ruiyansi Population. Sichuan J Zool, 29(1): 24–30
Stuart S. N., Chanson J. S., Cox N. A., Young B. E., Rodrigues A. S. L., Fischman D. L., Waller R. W. 2004. Status and trends of amphibian declines and extinctions worldwide. Science, 306: 1783–1786
Zane L., Bargelloni L., Patarnello T. 2002. Strategies for microsatellite isolation: A review. Mol Ecol, 11: 1–16

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