Jing ZHANG,Jie WANG and Jianping JIANG.Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla fissipes)[J].Asian Herpetological Research(AHR),2013,4(3):202-206.[doi:10.3724/SP.J.1245.2013.000202]
Click Copy

Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla fissipes)
Share To：

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2013 VoI.4 No.3
Page:
202-206
Research Field:
Original Article
Publishing date:
2013-09-25

Info

Title:
Development and Characterization of 15 Polymorphic Microsatellite Markers for a Highly Adaptive and Wide-range Frog (Microhyla fissipes)
Author(s):
Jing ZHANG;  Jie WANG and Jianping JIANG*
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China
Keywords:
Microhyla fissipes;  microsatellite;  genetic diversity;  population genetics
PACS:
-
DOI:
10.3724/SP.J.1245.2013.000202
Abstract:
In order to analyze population genetic structure at multiple spatial scales, microsatellite loci were developed for the ornamented pygmy frog (Microhyla fissipes), and 15 polymorphic microsatellite loci were successfully screened from 105 individuals, of which 82 from four populations distributed in the Sichuan Basin and 23 from the Sangzhi population in western Hunan. Five loci were found to deviate significantly from Hardy-Weinberg equilibrium in one to three populations, probably due to small sample size or null alleles. The average number of alleles in all loci was 8.5, ranging from 4 to 13, and the observed and expected heterozygosity ranged from 0.26 to 0.90 and 0.63 to 0.90, respectively. The Sangzhi population and the remaining four populations can be clearly separated using Bayesian clustering methods, showing that the genetic structure of M. fissipes was probably affected by the topography, especially mountain barriers. These polymorphic microsatellite loci could be used for further study on the landscape genetics of this highly adaptive and widely distributed species.

References:

Botstein D., White R. I., Skolnick M. 1980. Construction of a genetic linkage map in man using restriction fragment length polymorphisms. Am J Hum Genet, 32: 314–331
Dakin E. E., Avise J. C. 2004. Microsatellite null alleles in parentage analysis. Heredity, 93: 504–509
Evanno G., Regnaut S., Goudet J. 2005. Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol, 14: 2611–2620
Fei L., Hu S. Q., Ye C. Y., Huang Y. Z. 2009. Fauna Sinica, Amphibia, Vol. 3, Anura Ranidae. Beijing: Science Press (In Chinese)
Falush D., Stephens M., Pritchard J. K. 2003. Inference of population structure: Extensions to linked loci and correlated allele frequencies. Genetics, 164: 1567–1587
Falush D., Stephens M., Pritchard J. K. 2007. Inference of population structure using multilocus genotype data: Dominant markers and null alleles. Mol Ecol Notes, 7: 574–578
Holm S. 1979. A simple sequentially rejective multiple test procedure. Scand J Stat, 6: 65–70
Kalinowski S. T., Taper M. L., Marshall T. C. 2007. Revising how the computer program CERVUS accommodates genotyping error increases success in paternity assignment. Mol Ecol, 16: 1099–1106
Matsui M., Ito H., Shimada T., Ota H., Saidapur S. K., Khonsue W., Tanaka-Ueno T., Wu G. F. 2005. Taxonomic relationships within the Pan-Oriental narrow-mouth toad Microhyla ornata as revealed by mtDNA analysis (Amphibia, Anura, Microhylidae). Zool Sci, 22: 489–495
Noble D. W., Qi Y., Fu J. Z. 2010. Species delineation using Bayesian model-based assignment tests: A case study using Chinese toad-headed agamas (genus Phrynocephalus). BMC Evol Biol, 10: 197
Pritchard J., Stephens M., Donnelly P. 2000. Inference of population structure using multilocus genotype data. Genetics, 155: 945–959.
Qian X. W., Zhu M. Y. 2000. Karyotype diversity of different geographical population of Rana limmocharis boie, Rana j. japonica and Microhyla ornata. Hereditas, 22: 144–148
Rice W. R. 1989. Analyzing tables of statistical tests. Evolution, 43: 223–225
Rousset F. 2008. GENEPOP’007: A complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour, 8: 103–106
Rychlik W. 2007. OLIGO 7 Primer Analysis Software. Methods Mol Biol, 402: 35–59
Vos P., Hogers R., Bleeker M., Reijans M., Van de Lee T., Hornes M., Frijters A., Pot J., Peleman J., Kuiper M., Zabeau M. 1995. AFLP: A new technique for DNA fingerprinting. Nucl Acids Res, 23: 4407–4414
Van Osterhout C., Hutchinson W. F., Wills D. P. M., Shipley P. F. 2004. Micro-checker: Software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes, 4: 535–538
Wang B., Jiang J. P., Xie F., Li C. 2012. Postglacial Colonization of the Qinling Mountains: Phylogeography of the Swelled Vent Frog (Feirana quadranus). PLoS ONE, 7: e41579
Zane L., Bargelloni L., Patarnello T. 2002. Strategies for microsatellite isolation: A review. Mol Ecol, 11: 1–16
Zhou W. W., Wen Y., FU J. Z., Xu Y. B., Jin J. Q., Ding L., Min M. S., Che J., Zhang Y. P. 2012. Speciation in the Rana chensinensis species complex and its relationship to the uplift of the Qinghai-Tibetan Plateau. Mol Ecol, 21: 960–973

Memo

Memo:
-
Last Update: 2016-01-25