Shulin PAN,Ningxin DANG,Jishan WANG,et al.Molecular Phylogeny Supports the Validity of Polypedates impresus Yang 2008[J].Asian Herpetological Research(AHR),2013,4(2):124-133.[doi:10.3724/SP.J.1245.2013.00124]
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Molecular Phylogeny Supports the Validity of Polypedates impresus Yang 2008
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2013 VoI.4 No.2
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Original Article
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Molecular Phylogeny Supports the Validity of Polypedates impresus Yang 2008
Shulin PAN1 Ningxin DANG4 Jishan WANG3 Yitao ZHENG4 Dingqi RAO3 and Jiatang LI2*
1 School of Mining and Safety Engineering, Yibin University, Yibin 644007, Sichuan, China
2 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, Sichuan, China
3 Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
4 College of Life Sciences, Sichuan University, Chengdu 610064, Sichuan, China
Chinese Polypedates nucleotide sequence Polypedates leucomystax complex taxonomy
The taxonomy of the Asian tree frogs of the Polypedates leucomystax complex, a group of widespread and morphologically similar species, is very controversial. To ascertain the taxonomic status of these species, we investigated the historical relationships among representative samples based on ~ 2 kb of nucleotide sequences from the mitochondrial 12S rRNA, tRNAvaline, and 16S rRNA genes. Our phylogeny resolved five well supported lineages (A, B, C, D and E) in the P. leucomystax complex. Polypedates impresus from Yunnan, China, Polypedates cf. mutus and Polypedates cf. megacephalus from Guangxi and Yunnan, China, and Laos constructed Clade C, which is monophyletic. In order to recognize the unique position of this clade, we considered P. impresus in Clade C as a valid species. Following our phylogeny, Chinese Polypedates, corresponding to the other four clades, should include four species: P. mutus (Clade A); P. braueri (Clade B); P. impresus (Clade C) and P. megacephalus (Clade D). P. leucomystax (Clade E) is mainly distributed in the Malaysia, Indonesia and Philippines.


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