Chuanpeng NIE,Xiaobing WU,Yanyan LI and Juan ZHAO.ISSR Markers as a Tool for Assessing Genetic Diversity in the Chinese Alligator (Alligator sinensis)[J].Asian Herpetological Research(AHR),2012,3(4):310-315.[doi:10.3724/SP.J.1245.2012.00310]
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ISSR Markers as a Tool for Assessing Genetic Diversity in the Chinese Alligator (Alligator sinensis)
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2012 VoI.3 No.4
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Original Article
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ISSR Markers as a Tool for Assessing Genetic Diversity in the Chinese Alligator (Alligator sinensis)
Chuanpeng NIE1 2 Xiaobing WU1* Yanyan LI2 and Juan ZHAO1
1 College of Life Sciences, Anhui Normal University, Wuhu 241000, Anhui, China
2 Zoology Practical Skills Training Center, Fuyang Teachers College, Fuyang 236041, Anhui, China
inter simple sequence repeat (ISSR) genetic diversity Chinese alligator endangered species
Eight different inter simple sequence repeat (ISSR) markers were used as tools to investigate genetic variability and population differentiation in the Chinese alligator, Alligator sinensis, in this study. Eleven polymorphic bands (17.2%) out of a total of 64 were generated from 110 individuals in three populations. Analysis of molecular variation showed that most of the genetic variation (98.0%) occurred within the populations. Dendrogram relationship based on Nei’s unbiased genetic diversity illustrated that two breeding populations were genetically closely related. The Nm value of the study was 4.520, suggesting that high levels of gene flow existed and no differentiation appeared in the populations. In a reconstructed Neighbor-Joining tree, the haplotypes coming from the same populations did not gather as a class, suggesting the three populations had no apparent geographic pattern. This study shows that ISSR markers could be well applied as a feasible tool to assess genetic diversity in Chinese alligator individuals.


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