Vidya R. CHANDAVAR and Prakash R. NAIK.Immunocytochemical Localization of Glucagon and Insulin Cells in Eutropis carinata with Respect to Reproductive Cycle[J].Asian Herpetological Research(AHR),2011,2(2):103-109.[doi:10.3724/SP.J.1245.2011.00103]
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Immunocytochemical Localization of Glucagon and Insulin Cells in Eutropis carinata with Respect to Reproductive Cycle
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2011 VoI.2 No.2
Page:
103-109
Research Field:
Original Article
Publishing date:
2011-06-15

Info

Title:
Immunocytochemical Localization of Glucagon and Insulin Cells in Eutropis carinata with Respect to Reproductive Cycle
Author(s):
Vidya R. CHANDAVAR2 and Prakash R. NAIK1*
1 Endocrinology Laboratory, Department of Studies in Zoology, University of Mysore, Manasagangorti, Mysore 570006, India
2 Yuvaraja’s College, University of Mysore, Mysore 570006, India
Keywords:
endocrine cell pancreas serum glucose reproductive cycle skink
PACS:
-
DOI:
10.3724/SP.J.1245.2011.00103
Abstract:
The pancreas of Eutropis carinata was studied immunocytochemically for the presence and topographic distribution of glucagon and insulin cells during annual reproductive cycles. The study revealed paracrine arrangement of the two types of cells. The immunolocalized pancreatic cells revealed significant differences in mean numbers during different periods of reproduction. The numbers of glucagon-immunoreactive (GIR) and insulin-immunoreactive (IIR) cells were highest during the recrudescent period which corresponded with low serum glucose level. Between the two immunoreactive cells, the GIR cells were predominant. Morphological differences between the two cells were observed by electron microscopy after staining with uranyl acetate and lead citrate. Serum glucose showed that cyclic change was highest during the reproductive period.

References:

Bollen M., Keppens S., Stalmans W. 1998. Specific features of glycogen metabolism in the liver. Biochem J, 336: 19-31
Bonner-Weir S. 2000. Perspective: Postnatal pancreatic β cell growth. Endocrinology, 141(6): 1926-1929
Burry R. W. 2000. Specificity controls for immunocytochemical methods. J Histochem Cytochem, 48(2): 163-165
Chandavar V. R., Naik P. R. 2004. Variation in plasma glucose and pancreatic β cells in the turtle, Lissemys punctata (Order: Chelonia; Family: Trionychidae). Acta Zool, 85: 113-118
Chandavar V. R., Naik P. R. 2008. Immunocytochemical detection of glucagon and insulin cells in endocrine pancreas and cyclic disparity of plasma glucose in the turtle Melanochelys trijuga. J Biosci, 33: 239-247
Della R., Putti R. 1995. The endocrine pancreas of lacertids: An immunocytochemical study of the genera Pedioplanis and Meroles. Eur J Histochem, 39: 47-58
El-Salhy M., Grimelius L. 1981. Histological and immunohistochemical studies of the endocrine pancreas of lizards. Histochemistry, 72(2): 237-247
El-Salhy M., Abu-Sinna G., Wilander E. 1983. The endocrine pancreas of a squamate reptile, the desert lizard (Chalcides ocellatus). A histological and immunocytochemical investigation. Histochemistry, 78: 391-397
Foufelle F., Ferré P. 2002. New perspectives in the regulation of hepatic glycolytic and lipogenic genes by insulin and glucose: A role for the transcription factor sterol regulatory element binding protein-1c. Biochem J, 366: 377-391
Gomori G. 1941. Observations with differential stains on human islets of Langerhans. Am J Pathol, 17: 395-406
Hotchkiss R. D. 1968. The Periodic Acid Schiff (PAS) Technique. In Pearse A. G. E. (Ed.), Histochemistry Theoretical and Applied. Edinburgh: Churchhill, Living Stone, 659-660
Indian National Science Academy. 2000. Guidelines for care and use of animals in scientific research. New Delhi: Indian National Science Academy, 1-31
Johannessen J. V. 1978. Electron Microscopy in Human Medicine. Vol. 1. Instrumentation Techniques. New York: Mc Graw Hill International Book Company, 348 pp
Ku S. K., Lee H. S. 2004. The distribution and frequency of endocrine cells in the splenic lobe of grass lizard (Takydromus wolteri). An immunohistochemical study. Eur J Histochem, 48: 429-436
Lopez J. E., Chevarria M., Vazquez J. J. 1988. Histological and immunocytochemical study of the endocrine pancreas of the lizard Podarcis hispanica Steindachner, (Lacertidae). Gen Comp Endocr, 71: 212-228
Mausfeld P., Schmitz A. 2003. Molecular phylogeography, intraspeci?c variation and speciation of the Asian scincid lizard genus Eutropis, 1843 (Squamata: Reptilia: Scincidae): Taxonomic and biogeographic implications. Org Divers Evol, 3: 161-171
Miller M. R., Wurster D. H. 1956. Studies on the blood glucose and pancreatic islets of lizards. Endocrinology, 58: 114-120
Morescalchi A. M., Gaccioli M., Faraldi G., Tagliafierro G. 1997. The gastroenteric-pancreatic neuroendocrine system in two reptilian species, Chalcides chalcides and Zoonosaurus madascariensis (Sauridae). Eur J Histochem, 41: 29-40
Nozaki M., Miyata K., Oota Y., Gorbman A., Plisetskaya E. M. 1988. Different cellular distribution of two somatostatins in brain and pancreas of salmonids and their association with insulin and glucagon secreting cells. Gen Comp Endocr, 69: 267-280
Perez-Tomas R., Ballesta J., Pastor L. M., Madrid J. F., Polak J. M. 1989. Comparative immunohistochemical study of the gastroenteropancreatic endocrine system of three reptiles. Gen Comp Endocr, 76: 171-191
Putti R., Varano L., Cavagnuolo A., Laforgia V. 1991. The endocrine pancreas of Podarcis s. sicula Raf.: An immunocytochemical study at light and electron microscopic levels. Eur J Basic Appl His, 35: 145-159
Putti R., Della Rossa A., Varano L., Laforgia V., Cavagnuolo A. 1992. An immunocytochemical study of the endocrine pancreas in three genera of lacertids. Gen Comp Endocr, 87: 249-259
Rhoten W. B., Hall C. E. 1982. An immunocytochemical study of the cytogenesis of pancreatic endocrine cells in the lizard, Anolis carolinensis. Am J Anat, 163: 181-193
Trinder P. 1969. Determination of blood glucose using oxidase peroxidase system with a non carcinogenic chromogen. J Clin Pathol, 22: 158-161
Weesner F. M. 1960. General Zoological Microtechniques. Calcutta: The Williams and Wilkins Company, 1-230 pp
Yang H., Morrison C. M., Conlon J. M., Laybolt K., Wright J. R. 1999. Immunocytochemical characterization of the pancreatic islet cells of the Nile Tilapia (Oreochromis niloticus). Gen Comp Endocr, 114: 47-56

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