Fang YAN,Ke JIANG,Hongman CHEN,et al.Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China[J].Asian Herpetological Research(AHR),2011,2(2):61-71.[doi:10.3724/SP.J.1245.2011.00061]
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Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2011 VoI.2 No.2
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Original Article
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Matrilineal History of the Rana longicrus Species Group (Rana, Ranidae, Anura) and the Description of a New Species from Hunan, Southern China
Fang YAN1 Ke JIANG1 Hongman CHEN21 Ping FANG21 Jieqiong JIN1 Yi LI3 Shaoneng WANG4 Robert W. MURPHY1
1 State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming 650223, Yunnan, China
2 Laboratory for Conservation and Utilization of Bio-resources, Yunnan University, Kunming 650091, Yunnan, China
3 Jiemuxi National Nature Reserve, Yuanling 419600, Hunan, China
4 Maoershan National Nature Reserve, Guilin 541316, Guangxi, China
5 Centre for Biodiversity and Conservation Biology, Royal Ontario Museum, 100 Queen’s Park, Toronto, Ontario M5S 2C6, Canada
Rana jiemuxiensis Rana japonica group phylogeny genealogy DNA barcode cryptic species
Fieldwork in Jiemuxi National Nature Reserve, Hunan, China, discovered morphologically indistinguishable groups of brown frogs that bred at different, exclusive times of the year. A principal components analysis of morphometric data, molecular analyses, and the exclusive breeding season suggested the occurrence of two species. The population that breeds during the winter was found to be an undescribed species and was subsequently described as Rana jiemuxiensis sp. nov. The new cryptic species can be identified from its congeners at the same locality by having a different breeding season and a divergent DNA barcode. Six major lineages of brown frogs were resolved with high nodal support. Rana japonica, R. chaochiaoensis, R. omeimontis, and R. jiemuxiensis have independent maternal lineages. Rana hanlunica and R. maoershanensis have essentially identical maternal lineages and they appear to represent the same species. The validity of the species R. longicrus, R. zhenhaiensis, and R. culaiensis and their genealogical relationships are not resolved and deserve further study. The genealogy indicates that sympatric cryptic lineages probably widely exist in the R. longicrus group. This highlights the importance of future more fine-scaled samplings and the inclusion of nuclear genes.


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