YANG Baotian,LU Yuyan and LI Pipeng.Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene[J].Asian Herpetological Research(AHR),2010,1(2):97-102.[doi:10.3724/SP.J.1245.2010.00097]
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Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2010 VoI.1 No.2
Page:
97-102
Research Field:
Original Article
Publishing date:
2010-12-25

Info

Title:
Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene
Author(s):
YANG Baotian LU Yuyan and LI Pipeng*
Center for Chinese Endemic Herp-breeding and Conservation Research and Liaoning Key Laboratory of Evolution and Biodiversity, Shenyang Normal University, Shenyang 110034, Liaoning, China
Keywords:
Rana maoershanensis validity variation phylogeny 16S rDNA
PACS:
-
DOI:
10.3724/SP.J.1245.2010.00097
Abstract:
Rana maoershanensis found in Mt. Maoershan in Guangxi, China was reported as a new species in 2007, but there was no molecular data for this frog. The partial sequences (543 bp) of 16S rRNA gene from 12 specimens of 3 brown frog species (Rana hanluica, R. maoershanensis and R. chensinensis) were analyzed with 17 specimens of 9 species from GenBank. The nucleotide sequence divergence between R. maoershanensis and the other brown frog species were 4.5%?6.5%, with 22?30 nucleotide substitutions at this locus. The phylogenetic relationships based on MP, ML, and Bayesian inference indicate that the brown frogs from Southern China were diverged into three groups (Clades A, B and C). R. maoershanensis was clustered together a well-supported subclade (B-1). It is suggested that R. maoershanensis is a valid species.

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