YANG Baotian,LU Yuyan and LI Pipeng.Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene[J].Asian Herpetological Research(AHR),2010,1(2):97-102.[doi:10.3724/SP.J.1245.2010.00097]
Click Copy

Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene
Share To:

Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2010 VoI.1 No.2
Research Field:
Original Article
Publishing date:


Discussion on Validity of Rana maoershanensis Based on Partial Sequence of 16S rRNA Gene
YANG Baotian LU Yuyan and LI Pipeng*
Center for Chinese Endemic Herp-breeding and Conservation Research and Liaoning Key Laboratory of Evolution and Biodiversity, Shenyang Normal University, Shenyang 110034, Liaoning, China
Rana maoershanensis validity variation phylogeny 16S rDNA
Rana maoershanensis found in Mt. Maoershan in Guangxi, China was reported as a new species in 2007, but there was no molecular data for this frog. The partial sequences (543 bp) of 16S rRNA gene from 12 specimens of 3 brown frog species (Rana hanluica, R. maoershanensis and R. chensinensis) were analyzed with 17 specimens of 9 species from GenBank. The nucleotide sequence divergence between R. maoershanensis and the other brown frog species were 4.5%?6.5%, with 22?30 nucleotide substitutions at this locus. The phylogenetic relationships based on MP, ML, and Bayesian inference indicate that the brown frogs from Southern China were diverged into three groups (Clades A, B and C). R. maoershanensis was clustered together a well-supported subclade (B-1). It is suggested that R. maoershanensis is a valid species.


Che J., Pang J. F., Zhao E. M., Matsui M., Zhang Y. P. 2007. Phylogenetic relationships of the Chinese brown frogs (Genus Rana) inferred from partial mitochondrial 12S and 16S rRNA gene sequences. Zool Sci, 24: 71?80
Chen L. Q., Murphy R. W., Lathrop A., Ngo A., Orlov N. L., Ho C. T., Somorjai I. L. M. 2005. Taxonomic chaos in Asian ranid frogs: An initial phylogenetic resolution. Herpetol J, 15: 231?243
Darst C. R., Cannatella D. C. 2004. Novel relationships among hyloid frogs inferred from 12S and 16S mitochondrial DNA sequences. Mol Phylogenet Evol, 31: 462?475
Fei L., Hu S. Q., Ye C. Y., Huang Y. Z. 2009. Fauna Sinica (Amphibia: Anura Ranidae). Beijing: Science Press: 967?1645
Frost D. R. 2010. Amphibian species of the world: An online reference. Version 5.4 (8 April, 2010). from electronic database accessible at http://research.amnh.org/herpetology/amphibia/index. php. Am Museum Nat Hist. New York, USA
Frost D. R., Grant T., Faivovich J., Bain R. H., Haas A., Haddad C. F. B., Sá R. O. d., Channing A., Wilkinson M., Donnellan S. C., Raxworthy C. J., Campbell J. A., Blotto B. L., Moler P. E., Drewes R. C., Nussbaum R. A., Lynch J. D., Green D. M., Wheeler W. C. 2006. The amphibian tree of life. Bull Am Museum Nat Hist, 297: 1?370
Jang-Liaw N. H., Lee T. H. 2009. Intraspecific relationships of populations of the brown frog Rana sauteri (Ranidae) on Taiwan, inferred from mitochondrial cytochrome b sequences. Zool Sci, 26: 608?616
Jiang J. P., Xie F., Zheng Z. H. 2002. Phylogenetic relationships of Chinese brown frogs with discussion on the karyotype evolution. J Sichuan Univ (Natural Science Edition), 39: 85?89
Jiang J. P., Zhou K. Y. 2001a. Evolutionary relationships among Chinese ranid frogs inferred from mitochondrial DNA sequences of 12S rRNA gene. Acta Zool Sinica, 47: 38?44
Jiang J. P., Zhou K. Y. 2001b. Phylogenetic relationships of Chinese brown frogs inferred from mitochondrial DNA sequence of 12S ribosome RNA gene. Zool Res, 22: 27?32
Kimura M. 1980. A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. J Mol Evol, 16: 111?120
Kurabayashi A., Kuramoto M., Joshy H., Sumida M. 2005. Molecular phylogeny of the Ranid frogs from southwest India based on the mitochondrial ribosomal RNA gene sequences. Zool Sci, 22: 525?534
Li Y., Wu M., Wang X. L. 2004. Phylogenetic relationships of Hynobiidae based on sequences of mitochondrial 16S ribosomal RNA gene. Acta Zool Sinica, 50: 464?469
Lu Y. Y., Li P. P., Jiang D. B. 2007. A new species of Rana (Anura, Ranidae) from China. Acta Zootaxonomica Sinica, 32: 792?801
Lu Y. Y., Li P. P., Jiang D. B., Zhang J. H., Zhou L. Y., Wang S. N. 2006. A new record of amphibian in Guangxi — R. chaochiaoensis. Sichuan J Zool, 25: 267
Nylander J. A. A. 2004. MrModeltest v2. Program distributed by the author. Evolutionary Biology Centre, Uppsala University
Roelants K., Jiang J., Bossuyt F. 2004. Endemic ranid (Amphibia: Anura) genera in southern mountain ranges of the Indian subcontinent represent ancient frog lineages: Evidence from molecular data. Mol Phylogenet Evol, 31: 730?740
Ronquist F., Huelsenbeck J. P. 2003. MRBAYES 3: Bayesian phylogenetic inference under mixed models. Bioinformatics, 19: 1572?1574
Sambrook J., Russell D. 2001. Molecular cloning:A laboratory manual. Cold Spring Harbor, NY, USA: Cold Spring Harbor Lab Press
Sumida M., Kondo Y., Kanamori Y., Nishioka M. 2002. Interand intraspecific evolutionary relationships of the rice frog Rana limnocharis and the allied species R. cancrivora inferred from crossing experiments and mitochondrial DNA sequences of the 12S and 16S rRNA genes. Mol Phylogenet Evol, 25: 293?305
Sumida M., Ueda H., Nishioka M. 2003. Reproductive isolating mechanisms and molecular phylogenetic relationships among palearctic and oriental brown frogs. Zool Sci, 20: 567?580
Swofford D. L. 1998. PAUP: phylogenetic analysis using parsimony (and other methods). Sunderland, Massachusetts: Sinauer Associates, Inc
Tamura K., Dudley J., Nei M., Kumar S. 2007. MEGA4: Molecular evolutionary genetics analysis (MEGA) software version 4.0. Mol Biol Evol, 24: 1596?1599
Tanaka-Ueno T., Matsui M., Chen S. L., Takenaka O., Ota H. 1998. Phylogenetic relationships of brown frogs from Taiwan and Japan assessed by mitochondrial cytochrome b gene sequences (Rana: Ranidae). Zool Sci, 15: 283?288
Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. 1997. The ClustalX windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucl Acids Res, 25: 4876?4882
Yang Z. H., Rannala B. 1997. Bayesian phylogenetic inference using DNA sequences: A Markov Chain Monte Carlo method. Mol Biol Evol, 14: 717?724
Ye C. Y., Fei L., Matsui M. 1995. Taxonomic studies of Chinese Rana japonica Guenther. Acta Herpetol Sinica. Guiyang: Guizhou Science and Technology Press. 4?5: 82?87
Zhang Y. X., Wen Y. T. 2000. Amphibians in Guangxi. Guilin: Guangxi Normal University Press
Zhou F., Jiang A. W., Lu Z. 2005. A new record of amphibian species in Guangxi Rana zhenhaiensis. J Guangxi Agri and Biol Sci, 24: 248, 263


Last Update: 2016-03-15