Eunbin KIM,Hacheol SUNG,Donghyun LEE,et al.Nondestructive Skeletal Imaging of Hyla suweonensis Using Micro-Computed Tomography[J].Asian Herpetological Research(AHR),2017,8(4):235-243.[doi:10.16373/j.cnki.ahr.170069]
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Nondestructive Skeletal Imaging of Hyla suweonensis Using Micro-Computed Tomography
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

2017 VoI.8 No.4
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Nondestructive Skeletal Imaging of Hyla suweonensis Using Micro-Computed Tomography
Eunbin KIM1 Hacheol SUNG2 Donghyun LEE2 Geunjoong KIM2 Dongha NAM2*and Eungsam KIM2*
1 School of Biological Sciences and Biotechnology, Chonnam National University, 77, Yongbong-ro, Bukgu, Gwangju 61186, South Korea
2 Department of Biological Sciences, College of Natural Sciences, Chonnam National University, 77, Yongbong-ro, Bukgu, Gwangju 61186, South Korea
Hyla suweonensis Micro-computed tomography 3D skeletal structure Nondestructive imaging Endangered species Radiation dose.
We successfully obtained 3D skeletal images of Hyla suweonensis, employing a nondestructive method by applying appropriate anesthesia and limiting the radiation dose. H. suweonensis is a tree frog endemic to Korea and is on the list of endangered species. Previous studies have employed caliper-based measurements and two-dimensional (2D) X-ray imaging for anatomical analyses of the skeletal system or bone types of H. suweonensis. In this work we reconstructed three-dimensional (3D) skeletal images of H. suweonensis, utilizing a nondestructive micro-computed tomography (micro-CT) with a short scan and low radiation dose (i.e. 4 min and 0.16 Gy). Importantly, our approach can be applied to the imaging of 3D skeletal systems of other endangered frog species, allowing both versatile and high contrast images of anatomical structures without causing any significant damages to the living animal.


Boistel R., Pollet N., Tinevez J. Y., Cloetens P., Schlenker M. 2009. Irradiation damage to frog inner ear during synchrotron radiation tomographic investigation. J Electron Spectrosc, 170(1): 37–41
Borzée A., Didinger C., Jang Y. K. 2017. Complete mitochondrial genome of Dryophytes suweonensis (Anura Hylidae).?Mitochondr DNA Part B,?2(1): 5–6
Borzée A., Jang Y. K. 2015. Description of a seminatural habitat of the endangered Suweon treefrog Hyla suweonensis. Anim Cells Syst, 19(3): 216–220
Borzée A., Park S. Y., Kim A. B., Kim H. T., Jang Y. K. 2013. Morphometrics of two sympatric species of tree frogs in Korea: a morphological key for the critically endangered Hyla suweonensis in relation to H. japonica. Anim Cells Syst, 17(5): 348–356
Broeckhoven C., Plessis A., Roux S. G., Mouton P. L. F. N., Hui C. 2017. Beauty is more than skin deep: a non-invasive protocol for in vivo anatomical study using micro-CT. Methods Ecol Evol, 8(3): 358–369?
Emerson S. B. 1979. The ilio-sacral articulation in frogs: form and function. Biol J Linn Soc, 11(2): 153–168
Faivovich J., Haddad C. F., Garcia P. C., Frost D. R., Campbell J. A.,Wheeler W. C. 2005. Systematic review of the frog family Hylidae, with special reference to Hylinae: phylogenetic analysis and taxonomic revision. B Am Mus Nat Hist, 1–240
IUCN (International Union for Conservation of Nature). 2017. Internet references.
Jorgensen M., Reilly S. 2013. Phylogenetic patterns of skeletal morphometrics and pelvic traits in relation to locomotor mode in frogs. J Evolution Biol, 26(5): 929–943
Kim I. H., Ham C. H., Jang S. W., Kim E. Y., Kim J. B. 2012a. Determination of breeding season, and daily pattern of calling behavior of the endangered Suweon-tree frog (Hyla suweonensis). Korean J Herpetol, 4: 23–29 (In Korean with English abstract)
Kim I. H., Son S. H., Kang S. W., Kim J. B. 2012b. Distribution and habitat characteristics of the endangered Suweon-tree frog (Hyla suweonensis). Korean J of Herpetol, 4: 15–22 (In Korean with English abstract)
Klages J., Glaw F., K?hler J., Müller J., Hipsley C. A., Vences M. 2013. Molecular, morphological and osteological differentiation of a new species of microhylid frog of the genus Stumpffia from northwestern Madagascar. Zootaxa, 3717(2): 280–300
Krings M., Klein B., Heneka M. J., R?dder D. 2017. Morphological comparison of five species of poison dart frogs of the genus Ranitomeya (Anura: Dendrobatidae) including the skeleton, the muscle system and inner organs. PLOS ONE, 12(2): e0171669
Kuramoto M. 1980. Mating calls of treefrogs (genus Hyla) in the Far East, with description of a new species from Korea. Copeia, 100–108
Lambert S. M., Hutter C. R., Scherz M. D. 2017. Diamond in the rough: a new species of fossorial diamond frog (Rhombophryne) from Ranomafana National Park, southeastern Madagascar. Zoosyst Evol, 93: 143
Lee M. Y., Jeon H. S., Min M. S., An J. H. 2017. Sequencing and analysis of the complete mitochondrial genome of Hyla suweonensis (Anura: Hylidae). Mitochondr DNA Part B,?2(1): 126–127
Lessler M. A. 1959. Low-level X-ray damage to amphibian erythrocytes. Science, 129(3362): 1551–1553
Lessler M. A., Herrera F. M. 1962. Electron-microscope studies of x-ray damage to frog blood cells. Radiat Res, 17(2): 111–117
Nam D. H., Lee H. A., Kim E. B., Kim G. J., Kim E. S., Park C. G., Sung H. C., Lee D. H. 2017. Complete mitochondrial genome of a treefrog, Hyla sp.(Anura: Hylidae).?Mitochondr DNA Part B,?2(1): 221–222
Park S. Y., Jeong G. S., Jang Y. K. 2013. No reproductive character displacement in male advertisement
signals of Hyla japonica in relation to the sympatric H. suweonensis. Behav Ecol Sociobiol, 67(8): 1345–1355
Petrovi? T. G., Vukov T. D., Toma?evi? Kolarov N. 2017. Morphometric ratio analyses: Locomotor mode in anurans. C R Biol, 340(4): 250–257
Reilly S. M., Jorgensen M. E. 2011. The evolution of jumping in frogs: morphological evidence for the basal anuran locomotor condition and the radiation of locomotor systems in crown group anurans. J Morphol, 272(2): 149–168
Scherz M. D., Vences M., Rakotoarison A., Andreone F., K?hler J., Glaw F., Crottini A. 2016. Reconciling molecular phylogeny, morphological divergence and classification of Madagascan narrow-mouthed frogs (Amphibia: Microhylidae). Mol Phylogenet Evol, 100: 372–381
Suh J. H., Kim J. B., Min M. S., Suk H. Y., Yang S. Y. 1996. Skeletal character variation of 2 species of the genus Hyla. Bull Inst Basic Sci Inha Univ, 17: 41–50 (In Korean with English abstract)
Trube L. 1973. Evolutionary biology of the anurans:?contemporary research on major problems. Columbia, USA: University of Missouri Press. 65–132 pp
Trube L. 1993. The Skull, Volume 2: Patterns of Structural and Systematic Diversity. Chicago, USA: The University of Chicago Press. 255–343 pp
West G., Heard D., Caulkett N. 2014. Zoo animal and wildlife immobilization and anesthesia. Hoboken, USA: John Wiley and Sons. 308 pp
Yang S. Y., Park B. S. 1988. Speciation of the two species of the genus Hyla (anura) in Korea. Korean J Zool, 31: 11–20 (In Korean)
Yang S. Y., Park B. S., Son H. J. 1981. Species comparison of the genus Hyla in Korea. Bull Inst Basic Sci Inha Univ, 2: 75–83 (In Korean with English abstract)
Zhang M., Xiaoping C., Xiaohong C. 2016. Osteology of Quasipaa robertingeri (Anura: Dicroglossidae). Asian Herpetol Res, 7(4): 242–250
Zug G. R. 1972. Anuran locomotion: Structure and function. I. Preliminary observations on relation between jumping and osteometrics of appendicular and postaxial skeleton. Copeia, 613–624
Zug G. R. 1978.?Anuran locomotion-structure and function: Jumping performance of semiaquatic, terrestrial, and arboreal frogs. Smithsonian Institution Press. 1–21 pp


Last Update: 2017-12-25