Shouhong WANG,Lanying ZHAO,Lusha LIU,et al.A Complete Embryonic Developmental Table of Microhyla fissipes (Amphibia, Anura, Microhylidae)[J].Asian Herpetological Research(AHR),2017,8(2):108-117.[doi:10.16373/j.cnki.ahr.170006]
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A Complete Embryonic Developmental Table of Microhyla fissipes (Amphibia, Anura, Microhylidae)
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Asian Herpetological Research[ISSN:2095-0357/CN:51-1735/Q]

Issue:
2017 VoI.8 No.2
Page:
108-117
Research Field:
Publishing date:
2017-06-25

Info

Title:
A Complete Embryonic Developmental Table of Microhyla fissipes (Amphibia, Anura, Microhylidae)
Author(s):
Shouhong WANG1; 2;  Lanying ZHAO1; 2;  Lusha LIU1;  Dengwei YANG1;  Janak Raj KHATIWADA1; 2;  Bin WANG1 and Jianping JIANG1*
1 Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
2 University of Chinese Academy of Sciences, Beijing 100049, China
Keywords:
Microhylidae;  embryonic stage;  external characters;  morphogenesis
PACS:
-
DOI:
10.16373/j.cnki.ahr.170006
Abstract:
Access to embryonic developmental stages is essential basic work for understanding how organisms develop. In this study, seven egg clutches (range 209–564 eggs) of ornamented pygmy frog Microhyla fissipes (Amphibia, Anura, Microhylidae) were obtained from seven breeding pairs in laboratory. One egg clutch of them was observed for the embryonic development, and the staging table of normal development was constructed based on morphological and physiological characteristics. Forty-five developmental stages were defined for M. fissipes, and two major developmental periods were designated: 1) early embryonic development period (stages 1–28), from fertilization to operculum completion stage, lasted for 82.6 hours at water temperature (WT) 23–25oC; 2) larval development period (stages 29–45), from operculum completion to tail complete absorption stage, took 38 days at WT 22–26.5oC, showing that the embryos of this species develop rapidly. In addition, the tadpoles were transparent, which is similar to those in field. These characteristics suggest that M. fissipes would be a good model to study developmental biology, adaptive mechanisms from aquatic to terrestrial phases, environmental toxicology, and human disease.

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Last Update: 2017-06-25